Semiquantitation of Mouse Dendritic Cell Migration In Vivo Using Cellular MRI

Dekaban, Gregory A.; Snir, Jonatan; Shrum, Bradly; Chickera, Sonali de; Willert, Christy; Merrill, Mia; Said, Elias A.; Sékaly, Rafick Pierre; Foster, Paula J.; O’Connell, Peta J.

doi:10.1097/cji.0b013e318197b2a0

Cited by 44 publications

(78 citation statements)

References 46 publications

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“…These include detection of iron-labeled hepatocytes, macrophages, dendritic, cancer, and stem cells using T1-, T2-, and T2*-weighted imaging techniques and quantification using multiple MRI parameters including T2, R2, and R2* (20,(28)(29)(30)(31)(32)(33)(34)(35). It has been established qualitatively that the number of MPIO-labeled human breast cancer cells delivered to the brain is associated with the number of MRI signal voids (18).…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

et al. 2009

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The metastatic cell population, ranging from solitary cells to actively growing metastases, is heterogeneous and unlikely to respond uniformly to treatment. However, quantification of the entire experimental metastatic cell population in whole organs is complicated by requirements of an imaging modality with the large field of view and high spatial resolution necessary to detect both single cells and metastases in the same organ. Thus, it is difficult to assess differential responses of these distinct metastatic populations to therapy. Here, we develop a magnetic resonance imaging (MRI) technique capable of quantifying the full population of metastatic cells in a secondary organ. B16F1 mouse melanoma cells were labeled with micron-sized iron oxide particles (MPIO) and injected into mouse liver via the mesenteric vein. Livers were removed immediately or at day 9 or 11, following doxorubicin or vehicle control treatment, and imaged using a 3T clinical magnetic resonance scanner and custom-built gradient coil. Both metastases (>200 μm) and MPIO-labeled single cells were detected and quantified from MR images as areas of hyperintensity or hypointensity (signal voids), respectively. We found that 1 mg/kg doxorubicin treatment inhibited metastasis growth (n = 11 per group; P = 0.02, t test) but did not decrease the solitary metastatic cell population in the same livers (P > 0.05). Thus, the technique presented here is capable of quickly quantifying the majority of the metastatic cell population, including both growing metastases and solitary cells, in whole liver by MRI and can identify differential responses of growing metastases and solitary cells to therapy. [Cancer Res 2009;69(21):8326-31]

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Section: Discussionmentioning

confidence: 99%

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

et al. 2009

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“…Here the subcutaneous injection of DC into the footpad resulted in the migration of DC to the draining popliteal lymph node, which was detected as signal loss in the node 2 days after the injection. This signal loss was no longer apparent at 7 days post injection, nor were DC detected by histology, indicating that the DC, which have a lifetime of 4-7 days, had been cleared [64].…”

Section: Discussionmentioning

confidence: 92%

The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury

González-Lara

Hofstetrova

et al. 2010

Mol Imaging Biol

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“…[3][4][5] Iron oxide particles may also be used for noninvasive tracking of the migration and biodistribution of immune cells. 6 Cellular tracking typically involves labeling the cells ex vivo and subsequently implanting [7][8][9][10] or systemically 11 administering particles into the living body. These labeling methods are useful for observing the migration of immune cells toward a target, such as a tumor, and their subsequent accumulation.…”

Section: Introductionmentioning

confidence: 99%

MR Contrast in Mouse Lymph Nodes with Subcutaneous Administration of Iron Oxide Particles: Size Dependency

et al. 2011

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Purpose: We investigated the spatiotemporal changes in signal in draining lymph nodes of mice to ascertain the size-dependent eŠects of variously sized particles of iron oxide used to enhance magnetic resonance (MR) lymphography.Materials and Methods: We injected iron oxide particles of 50-, 100-, 200-, or 1,000-nm diameter into the footpads of individual mice and obtained sequential MR images of the popliteal and inguinal lymph nodes with 11.7 tesla up to 6 weeks after particle administration.Results: Up to 30 min after administration of particles smaller than 100 nm, we observed a marked reduction in signal in the popliteal node that spread from the periphery atˆrst observation toward the center of the node in subsequent measurements and persisted up to 6 weeks. In contrast, 1,000-nm particles caused dot-like areas of hypointensity in the popliteal lymph node, primarily in the inner portion, that appeared after 2 days. In the inguinal lymph nodes, signal changes occurred after 2 days for 50-and 100-nm particles and after one week for 1,000-nm particles. For 1,000-nm particles, areas of hypointensity were visible in the inner portion and not the periphery of the inguinal lymph node up to 6 weeks. In this study, we demonstrate the strong dependence of MR imaging contrast in draining lymph nodes on the size of the particle-shaped contrast agents injected subcutaneously. Particle size represented passive and active targeting eŠects, so micron-sized particles produced delayed enhancement.Conclusion: Choosing the size of iron oxide particles for MR imaging contrast depends on the objective of observation, such as identifying the morphology or migration of immune cells in the lymph node.

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Semiquantitation of Mouse Dendritic Cell Migration In Vivo Using Cellular MRI

Cited by 44 publications

References 46 publications

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

Three-Dimensional Imaging and Quantification of Both Solitary Cells and Metastases in Whole Mouse Liver by Magnetic Resonance Imaging

The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury

MR Contrast in Mouse Lymph Nodes with Subcutaneous Administration of Iron Oxide Particles: Size Dependency

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