The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury

González-Lara, Laura E.; Xu, Xiaoyun; Hofstetrova, Klara; Pniak, Anna; Chen, Yuhua; McFadden, Catherine; Martinez-Santiesteban, Francisco M.; Rutt, Brian K.; Brown, Arthur; Foster, Paula J.

doi:10.1007/s11307-010-0393-y

Cited by 42 publications

(38 citation statements)

References 63 publications

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“…Several studies have pointed out that the signal loss produced by iron-labeled cells can greatly overestimate the presence of the viable transplanted cell population. 29,[42][43][44] The signal void caused by the presence of iron-labeled cells is similar to hypointense signal produced by bone, air, and hemorrhage. This is a major hurdle to the use of MRI to reliably identify iron-labeled cells after their transplantation.…”

Section: F-versus Iron-labeled Cellsmentioning

confidence: 94%

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Ribot¹,

Gaudet²,

Chen³

et al. 2014

IJN

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Mesenchymal stem cells (MSC) are used to restore deteriorated cell environments. There is a need to specifically track these cells following transplantation in order to evaluate different methods of implantation, to follow their migration within the body, and to quantify their accumulation at the target. Cellular magnetic resonance imaging (MRI) using fluorine-based nanoemulsions is a great means to detect these transplanted cells in vivo because of the high specificity for fluorine detection and the capability for precise quantification. This technique, however, has low sensitivity, necessitating improvement in MR sequences. To counteract this issue, the balanced steady-state free precession (bSSFP) imaging sequence can be of great interest due to the high signal-to-noise ratio (SNR). Furthermore, it can be applied to obtain 3D images within short acquisition times. In this paper, bSSFP provided accurate quantification of samples of the perfluorocarbon Cell Sense-labeled cells in vitro. Cell Sense was internalized by human MSC (hMSC) without adverse alterations in cell viability or differentiation into adipocytes/osteocytes. The bSSFP sequence was applied in vivo to track and quantify the signals from both Cell Sense-labeled and iron-labeled hMSC after intramuscular implantation. The fluorine signal was observed to decrease faster and more significantly than the volume of iron-associated voids, which points to the advantage of quantifying the fluorine signal and the complexity of quantifying signal loss due to iron.

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Section: F-versus Iron-labeled Cellsmentioning

confidence: 94%

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Ribot¹,

Gaudet²,

Chen³

et al. 2014

IJN

Self Cite

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show abstract

“…Cellular MRI is most often employed to image cells after their transplantation. For example, many different types of stem and progenitor cells have been successfully tracked in vivo with MRI using iron oxide and studies show there is minimal impact on cell function or phenotype at a wide range of iron loading levels [47][48][49]. Very few groups have used cellular MRI to study cancer cells because the iron label is Fig.…”

Section: Imaging Dormant Cancer Cellsmentioning

confidence: 99%

Brain metastases from breast cancer: lessons from experimental magnetic resonance imaging studies and clinical implications

2013

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Breast cancer that has metastasized to the brain presents difficult clinical challenges. This diagnosis comes with high mortality rates, largely due to complexities in early detection and ineffective therapies associated with both dormancy and impermeability of the blood-brain barrier (BBB). Magnetic resonance imaging (MRI) is the current gold standard for diagnosis and assessment of brain tumors. It has been used clinically to investigate metastatic development as well as monitor response to therapy. Here, we describe preclinical imaging strategies that we have used to study the development of brain metastases due to breast cancer. Using this approach, we have identified three subsets of metastatic disease: permeable metastases, nonpermeable metastases, and solitary, dormant cancer cells, which likely have very different biology and responses to therapy. The ability to simultaneously monitor the spatial and temporal distribution of dormant cancer cells, metastatic growth, and associated tumor permeability can provide great insight into factors that contribute to malignant proliferation. Our preclinical findings suggest that standard clinical detection strategies may underestimate the true metastatic burden of breast cancer that has metastasized to the brain. A better understanding of true metastatic burden in brains will be important to assist in the development of more effective chemotherapeutics-particularly those targeted to cross the BBB-as well as detection of small nonpermeable metastases.

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“…Typical examples are supermagnetic iron oxide (SPIO) particle by using MRI4) and radionuclides by using positron emission tomography or single-photon emission computed tomography 59). In particular, many studies which use stem cells labeled with SPIO particle has been discussed in many animal experiments, clinical trials 7202473). These studies are likely to be a future standard of stem cell treatment because it allows the in vivo identification of implanted stem cells.…”

Section: Evaluation Tools Of the Therapeutic Effectmentioning

confidence: 99%

Current Concept of Stem Cell Therapy for Spinal Cord Injury: A Review

Jeon

2016

Korean J Neurotrauma

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Spinal cord injury (SCI) is a catastrophic condition associated with significant neurological deficit, social, and financial burdens. Over the past decades, various treatments including medication, surgery, and rehabilitation therapy for SCI have been performed, but there were no definite treatment option to improve neurological function of patients with chronic SCI. Therefore, new treatment trials with stem cells have been studied to regenerate injured spinal cord. Among various types of stem cells, bone marrow derived mesenchymal stem cells is highly expected as candidates for the stem cell therapy. The result of the current research showed that direct intramedullary injection to the injured spinal cord site in subacute phase is most effective. Neurological examination, electrophysiologic studies, and magnetic resonance imaging are commonly used to assess the effectiveness of treatment. Diffusion tensor imaging visualizing white matter tract can be also alternative option to identify neuronal regeneration. Despite various challenging issues, stem cell therapy will open new perspectives for SCI treatment.

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The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury

Cited by 42 publications

References 63 publications

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

In vivo MR detection of fluorine-labeled human MSC using the bSSFP sequence

Brain metastases from breast cancer: lessons from experimental magnetic resonance imaging studies and clinical implications

Current Concept of Stem Cell Therapy for Spinal Cord Injury: A Review

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