Seminal plasma proteins as potential biomarkers for sperm motility and velocities

Gaitskell‐Phillips, Gemma; Martín-Cano, Francisco E.; Ortiz-Rodríguez, J.M.; Silva-Álvarez, Eva da; Masot, Javier; Redondo, E.; Gil, María Concepción Robles; Ortega-Ferrusola, Cristina; Peña, Fernando J.

doi:10.1016/j.theriogenology.2021.10.007

Cited by 10 publications

(11 citation statements)

References 26 publications

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“…However, shot-gun proteomic studies on equine seminal plasma are scarce. Although the few existing studies have provided interesting findings [6,7,73], reinforcing the idea of the important role of seminal plasma in sperm functionality and the interaction with the mare's reproductive tract.…”

Section: Proteomics Of Seminal Plasmamentioning

confidence: 86%

Proteomics is advancing the understanding of stallion sperm biology.

PEÑA,

Cano,

BECERRO-REY

et al. 2024

Preprint

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The mammalian ejaculate is very well suited for proteomics studies. As such, investigations on the sperm proteomics are offering a huge amount of new information on the biology of the spermatozoa. Among domestic animals, horses represent a special interest species, in which reproductive technologies and an important market of genetic material has growth exponentially in the last decade. Investigations using proteomic approaches have been conducted in recent years, showing that proteomics is a potent tool to dig into the biology of the stallion spermatozoa. The aim of this review is to present an overview of the research conducted, and how these studies have improved our knowledge of the stallion sperm biology. The main outcomes of the research conducted so far have been an improved knowledge of the metabolism, and its importance of sperm functions, the impact of different technologies in the sperm proteome, and the identification of potential biomarkers. Moreover, proteomics of the seminal plasma and phosphoproteomics are identified as areas of major interest.

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Section: Proteomics Of Seminal Plasmamentioning

confidence: 86%

Proteomics is advancing the understanding of stallion sperm biology.

PEÑA,

Cano,

BECERRO-REY

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…The same protocol as described in [3] , [4] , [5] , [6] , [7] , [8] , [9] was used. Stallion ejaculates were washed in PBS (600gx 10’), then the pellet formed by the spermatozoa was frozen immediately in liquid nitrogen and kept frozen at -80 °C.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The separation and analysis were conducted in an Agilent 1290 Infinity II Series UHPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with an automated multisampler module and a High Speed Binary Pump, coupled to a Mass Spectrometer (Agilent 6550 Q-TOF, Agilent Technologies, Santa Clara, CA, USA) using an Agilent Jet Stream Dual electrospray (AJS-Dual ESI) interface, controlled by the MassHunter Workstation Data Acquisition software (Agilent Technologies, Rev. B.06.01), following previously published protocols [ 2 , 4 , 5 , 9 ]. The digested proteins were injected onto an Agilent Advance Bio Peptide Mapping HPLC column (2.7 μm, 150 × 2.1 mm, Agilent technologies), at 55 °C, at a flow rate of 0.4 ml/min.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Data were analyzed using the Spectrum Mill MS Proteomics Workbench (Rev B.04.01, Agilent Technologies, Santa Clara, CA, USA) as described in previous publications from our laboratory [ 5 , 6 ]. The raw data were extracted under default conditions: selection of non fixed or variable modifications; [MH]+ 50–10,000 m/z; maximum precursor charge +5; retention time and m/z tolerance ± 60 s; minimum signal-to-noise MS (S/N) 25; finding 12 C signals.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Dataset of the sperm proteome of stallions with different motility

et al. 2022

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“…Furthermore, 55 DEPs were identified in the seminal plasma between the HQS-vs-LQS. Recently, proteomic studies in mammals and poultry seminal plasma have identified some important proteins associated with the semen quality ( Gaitskell-Phillips et al, 2021 ; Jia et al, 2021 ; Słowińska et al, 2021 ). These results indicated that the seminal plasma proteins play a major role in regulating semen quality of drakes.…”

Section: Disscusionmentioning

confidence: 99%