A comparative proteomic study of high and low semen quality seminal plasma in drakes

Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

“…In the present study, the semen (collected by abdominal massage), cloacal swabs (10 sterile cotton swabs were wiped cloaca after soaking in sterile phosphate buffered saline solution), and environmental swabs (3 sterile gauzes were wiped breeding cage after soaking in sterile phosphate buffered saline solution) from the 20 drakes (2 of them were in moult and could not collect semen, so they were discarded) were collected at a 3-d interval from 400 to 408 d of age. Meanwhile, 20 µL of each semen sample was absorbed after measuring volume to assess sperm viability, sperm concentration, total sperm number, and morphological abnormal sperm according to the methods we previously reported ( Tang et al, 2022 ), and the procedures were detailed in below: 1) 10 µL semen was mixed with 200 µL of 1% trypan blue solution (Solarbio, Beijing, China) and incubated for 15 min. Sperm viability was assessed by placing 10 µL of stained semen on a preheated slide (37℃) and a phase contrast microscope (Olympus, Tokyo, Japan) at 400 × was used to observe the color about 200 sperm.…”

“… Habibullah et al (2015) pointed out that compared to natural mating, AI technology could produce more chicks per hen; and the actual mating capability of each rooster could be increased tenfold through AI technology ( Kharayat et al, 2016 ; Santiago-Moreno et al, 2016 ). Recently, breeders have also started applying this technology in the duck industry ( Ouyang et al, 2021 ; Tang et al, 2022 ). However, in production applications, AI in ducks appears to reduce egg quality and increase the likelihood of health issues such as rectocele, peritonitis, salpingitis, and ascites in female ducks.…”

Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis

“…Next, elution buffer (0.1% formic acid, 70% acetonitrile) was added. The eluents of each sample were collected and lyophilized ( Tang et al, 2022 ).…”

“…The tryptic peptides were analyzed using an EASY-nLCTM 1200 ultra-high pressure liquid chromatography system ( UHPLC ) system (ThermoFisher, Wilmington, DE) coupled to an Orbitrap Exploris 480 mass spectrometer (ThermoFisher, Wilmington, DE) according to the previous literature with slight modification ( Tang et al, 2022 ). The EASY-nLCTM 1200 UHPLC system was equipped with a home-made C18 Nano-Trap column (4.5 cm × 75 μm, 3 μm) and a home-made analytical column (25 cm × 150 μm, 1.9 μm).…”

Influence of Technological Stages of Preparation of Rooster Semen for Short-Term and Long-Term Storage on Its Quality Characteristics

“…Seminal plasma is a key body fluid that modulates sperm function in all animal species, but the role of seminal plasma in storing avian sperm in vitro remains largely a subject of controversy, since both inhibitory and stimulatory effects of its influence on the efficiency of artificial insemination and both short-term and long-term storage of sperm have been found [ 8 , 9 ]. Recent studies in the development of biochemical and ohmic assessment methods in sperm biology have improved knowledge of the initial components and underlying mechanisms [ 10 , 11 ]. In studies of the composition of the seminal plasma of roosters, the presence of specific proteins ovotransferrin and gallinacin-9, which have an antibacterial function, has been revealed [ 12 , 13 ].…”