Seminal plasma caspase 3, cytochrome c and total antioxidant capacity in
 oligospermic males and association with sperm indices

Emokpae, Abiodun Mathias; Chima, Happy Ngozi; Ahmed, Muhammed

doi:10.5455/jeim.091116.or.164

Cited by 4 publications

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“…A significantly higher levels of caspase 3 and DNA fragmentation as well as positive correlation were observed in male subjects evaluated for infertility with >45 yr as age threshold by Ariondo and co-workers (16). These authors suggested that senescence may be associated with significantly higher levels of apoptotic markers in seminal plasma of men with proven fertility and infertility.…”

Section: Discussionmentioning

confidence: 83%

Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study

Emokpae

Chima

2018

IJRM

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Background: Male senescence may affect testicular function, sperm indices and generation of high levels of oxidants and apoptosis. Objective: This study evaluates the effect of male age on the expression of some apoptosis and oxidative stress markers in seminal fluid of males investigated for infertility in a tertiary health institution in Nigeria. Materials and Methods:In this cross-sectional study, 122 men aged 20-60 yr who were investigated for infertility and were stratified according to age into four groups. Seminal plasma caspase 3, cytochrome C, and total antioxidant capacity (TAC) were assayed by ELISA technique, while manual semen analysis was performed according to WHO standard. Results: Seminal caspase 3, and cytochrome C activity increased while TAC and sperm indices decreased with increasing age. Cytochrome C (r=0.288; p=0.002) and caspase 3 (r=0.250; p=0.05) correlated significantly with age in normospermia while cytochrome C (r=0.314; p=0.02), caspase 3 (r=0.268; p=0.05), TAC (r=-0.342; p=0.01) and morphology percentage (r=-0.414; p=0.002) correlated with age in oligospermic infertile males. Conclusion: The measured apoptotic markers increased with increasing age while TAC and sperm indices decreased with increasing age of subjects evaluated. Although the levels of measured apoptosis and oxidative stress markers correlated with age in normozospermia, the effect on sperm indices was severe among oligospermia compare to normozospermia. Therefore, these markers may be assayed in aged men attending fertility clinics.

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Section: Discussionmentioning

confidence: 83%

Effect of senescence on some apoptosis and oxidative stress markers in infertile normozospermic and oligospermic men: A cross-sectional study

Emokpae

Chima

2018

IJRM

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“…These could result in gene mutation and carcinogenesis [45,48]. Our group previously reported high levels of seminal plasma caspase 3, cytochrome c and low total antioxidant capacity in infertile men in Nigeria [49]. Defective mitochrondrial dependent apoptotic signaling pathway may be an important contributing factor to infertility [49][50][51][52][53][54] .…”

Section: Extrinsic Factorsmentioning

confidence: 99%

“…Our group previously reported high levels of seminal plasma caspase 3, cytochrome c and low total antioxidant capacity in infertile men in Nigeria [49]. Defective mitochrondrial dependent apoptotic signaling pathway may be an important contributing factor to infertility [49][50][51][52][53][54] . The control spermatogenesis is nurtured and aided by Sertoli cell and in the presence of large number of spermatozoa with damaged DNA, the Sertoli cells express FasL which induces sperm cell apoptosis by Fas/FasL pathway in order to maintain equilibrium necessary for normal spermatogenesis [55,56].…”

Section: Extrinsic Factorsmentioning

confidence: 99%

“…High levels of DNA fragmentation and active caspase 3 were reported in testes of men with Sertoli cell only syndrome and maturation arrest [57] . We earlier reported lower levels of total antioxidant capacity in infertile than control male subjects [49] . The increased generation of ROS often observed in older men [37][38][39], may be due to several factors and include routine medical prescription and environmental pollutants [58].…”

Section: Extrinsic Factorsmentioning

confidence: 99%

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