Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation

Aurich, Jörg; Kühne, A.; Hoppe, Heinrich C.; Aurich, Christine

doi:10.1016/s0093-691x(96)00237-3

Cited by 120 publications

(95 citation statements)

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“…However, SP of stallions with different freezability added to ejaculates before semen processing influenced post-thaw sperm quality (Aurich et al, 1996). In this study, there was no difference between SP of the six donor stallions at any concentration, which is in contrast to hypothesis (2) and previous studies, where the effect of SP on ejaculated spermatozoa has been reported to vary between stallions (Aurich et al, 1996;Katila et al, 2004;Akcay et al, 2006). Therefore, interaction between SP and ejaculated, and SP and epididymal spermatozoa may differ.…”

Section: (A) (B) (C) (D)mentioning

confidence: 99%

“…In most studies, short exposure to high concentrations of SP and storage (cooled or frozen) at low concentrations of SP are recommended (Varner et al, 1987;Jasko et al, 1992;Braun et al, 1994). In the study of Aurich et al (1996), SP was incubated for 10 min, indicating that membrane changes in spermatozoa occur even if there is just a short period of contact with SP (Aurich et al, 1996). Therefore, a short period of incubation immediately before insemination could be beneficial for the semen dose.…”

Section: <00001mentioning

confidence: 99%

“…However, in that study, only one stallion was used as SP donor. Seminal plasma from different stallions influences the quality of frozen-thawed ejaculated spermatozoa (Aurich et al, 1996). In addition, concentration of SP seems important for sperm motion characteristics after centrifugation (Braun et al, 1994) as well as in spermatozoa selected by colloid centrifugation [single layer centrifugation (SLC); Morrell et al, 2010Morrell et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

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Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

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SUMMARYPreservation of epididymal spermatozoa is important to save genetic material of endangered species and breeds, or in case of unexpected injury, which will end the breeding career of valuable sires. Seminal plasma (SP) influences sperm quality in a dosedependent manner and its addition to preserved semen immediately before insemination may be beneficial for sperm fertility. Increased plasma membrane stability of epididymal spermatozoa reduces freezing injury of cells, and the addition of SP after freezing and thawing might have activating and protecting effects on spermatozoa within the female genital tract. In this study, epididymal spermatozoa were harvested by retrograde flush of the epididymal cauda immediately after routine castration and frozen. Seminal plasma was collected from other six stallions. Homologous SP (SP from the same species, but from a different animal) was added to frozen-thawed epididymal spermatozoa at concentrations of 0, 5, 20, 50 and 80% SP. Addition of SP increased sperm motility and influenced kinematic values in a dose-dependent manner (p < 0.05). Motility improved at concentrations of 20 and 50% SP, but did not further increase at 80% SP. There was no difference in sperm motility among SP from six different donor stallions regardless of the concentrations of SP (p > 0.05). Total and progressive motility of ten frozen-thawed epididymal spermatozoa samples collected from different stallions after dilution with extender and 5, 20, 50 or 80% SP differed significantly (p < 0.05). In conclusion, addition of homologous SP to frozen-thawed stallion epididymal spermatozoa immediately improved motility in a dose-dependent manner regardless of semen quality of SP donor stallions. This might positively influence fertility when SP is added before insemination. Moreover, there seems to be a threshold level of SP concentration for optimal improvement of sperm motility.

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Section: (A) (B) (C) (D)mentioning

confidence: 99%

Section: <00001mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

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“…Some parameters such as extenders, dilution ratios and storage temperature differences have been reported to effect success by different researchers [3][4][5][6][7][8]10,11] . Similar to this, the research determined that in examinations performed after dilution ratio or spermatozoa concentration does not effect spermatological properties.…”

Section: Discussionmentioning

confidence: 99%

“…Removal of seminal plasma by centrifugation before cryopreservation causes a premature capacitation reaction when the semen is frozen. For that reason, spermatozoa are unable to undergo the acrosome reaction and complete fertilisation [3,9,10] . Different diluents (INRA 82, lactose-EDTA, skimmed milk, skimmed milk-glucose, sugar based or ionic diluents), different cryoprotectants (Glycerol, Ethylene glycol, DMSO), different packaging methods (ampoule, vial, 0.25 and 0.5 ml sequins, 4 ml macrotube, 2.5 ml flat macrotube) and storage methods (short and long-term) were used for storage of stallion semen [10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi

Tirpan¹,

Akçay²,

Şen³

et al. 2015

Kafkas Univ Vet Fak Derg

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This study was conducted to evaluate the effects of different temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. Ejaculates from four warm-blooded stallions were used in this study. Ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. Only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. Semen was diluted with INRA 96 extender to final concentrations of 50, 100, 200 and 400x10 6 sp/ml. Diluted semen were stored at different temperatures (2, 4 or 8°C) for 24-48 h. After 24 h and 48 h cooled storage, spermatozoon motility, abnormal spermatozoon ratio and membrane integrity were evaluated. According to results the highest motility values (52.5% and 55%) were determined at 50 and 100 x10 6 sp/ml concentration at 4°C after 24 h cooled storage. After 48 h of storage, the highest motility was obtained from semen stored at 8°C. Abnormal spermatozoon and membrane integrity values were not significantly different after cooled storage. Consequently, it was determined that semen with 50 and 100 million spermatozoa/ml concentration stored at 4°C for 24 h and semen with 50, 100, 200, 400 million spermatozoa/ml concentration stored at 8°C for 48 h gave better results with respect to motility than the others. Keywords: Stallion semen, Doses, Temperature, Short-term preservation, In vitro evaluation of semen Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi ÖzetBu araştırma, farklı doz ve sıcaklıkların (24-48 saat) aygır spermatozoonlarının yaşam kabiliyetleri üzerine etkisini ortaya koymak amaçlanmıştır. Çalışmada dört sıcakkanlı aygırdan alınan ejekülatlar kullanılmıştır. Ejekülatların alınması için açık uçlu suni vagina kullanılmış ve bu yolla sperma fraksiyonlar halinde cam kaplara alınmıştır. Deney süresince sadece 2. ve 3. fraksiyonlar (spermatozoondan zengin fraksiyonlar) çalışmada kullanılmıştır. Sperma INRA 96 sulandırıcısı ile final yoğunluk 50, 100, 200, 400x10 6 spermatozoa/ ml (sp/ml) olacak şekilde sulandırılmıştır. Sulandırılan sperma 2°C, 4°C ve 8°C lik sıcaklıklarda 24-48 saat süreler ile saklanmıştır. 24-48 saatlik saklama sonrası spermatozoon motilitesi, anormal spermatozoa ve membran bütünlüğü yönünden muayene edilmiştir. Elde edilen sonuçlara göre kısa süreli saklanan spermada 24. saat sonunda en yüksek motilite değerleri (%52.5 ve %55) 4°C'de 50 ve 100 milyon yoğunlukta elde edilmiştir, 48. saat sonunda ise en yüksek motilite değerleri 8°C'de saklanan spermadan elde edilmiştir. Anormal spermatozoa ve membran bütünlüğü değerleri farklılık göstermemiştir. Sonuç olarak, 4°C'de 50 ve 100 milyon dozlarında 24 saat saklamanın ve 8°C'de 50, 100, 200 ve 400 milyon dozlarında 48 saat saklamanın diğerlerine göre daha iyi sonuçlar verdiği tespit edilmiştir.

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Improvement of Cryopreserved Ram Sperm Heterogeneity and Viability by Addition of Seminal Plasma

OLLERO,

CEBRIAN‐PEREZ,

MUIÑO‐BLANCO

1997

Journal of Andrology

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The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen‐thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen‐quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two‐phase system analysis. All samples used for this study were obtained from Salz rams by an artificial vagina and frozen according to the Fiser method. Sperm samples, consisting of either raw or washed semen, were frozen in the presence of either whole ram seminal plasma (RSP) or >10 kDa seminal plasma fraction. Two control samples were considered: one diluted in Fiser's extender and an undiluted sample. Cell‐membrane integrity (viability), hypoosmotic swelling (HOS) test, and sperm motility were assessed in all cases after collection, before freezing (at 5°C), after thawing, and after removing the diluent before CCCD analysis. Our results show that sperm cell partition behavior was dependent on the sperm treatment and that the freezing‐thawing process accounted for a clear loss of sperm heterogeneity and membrane integrity. These losses were less apparent when seminal plasma had been removed from semen samples before freezing. In addition, washed semen frozen in the presence of either >10 kDa seminal plasma fraction or whole re‐added seminal plasma maintained a higher rate of sperm heterogeneity and viability.

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Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation

Cited by 120 publications

References 10 publications

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

Farklı Doz ve Sıcaklıklarda Kısa Süreli Saklanan Aygır Spermasının in vitro Değerlendirilmesi

Improvement of Cryopreserved Ram Sperm Heterogeneity and Viability by Addition of Seminal Plasma

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