Abstract:This study was conducted to evaluate the effects of different temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. Ejaculates from four warm-blooded stallions were used in this study. Ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. Only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. Semen was diluted with INRA 96 extender to final conce… Show more
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