Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker

Hanaki, Ken-Ichi; Momo, Asami; Oku, Taisuke; Komoto, Atsushi; Maenosono, Shinya; Yamaguchi, Yukio; Yamamoto, Kenji

doi:10.1016/s0006-291x(03)00211-0

Cited by 313 publications

(228 citation statements)

References 22 publications

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“…First demonstrated by Chan & Nie close to a couple of decades back, the most popular mercapto alkyl acid based ligand is mercaptopropionic acid (MPA) [2,17]. The others include mercaptohexanoic acid (MHA) [21], mercaptoundecanoic acid (MUA) [22] [4,23] and thiol-containing amino acids such as cysteine [23] and D-penicillamine [24,25] have also been used for ligand exchange. These ligands bind to the QD surface via the thiol functionality and terminate in a hydrophilic amine group, which can be further used for conjugation.…”

Section: Small Molecular Weight Ligandsmentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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Semiconductor nanoparticles particularly quantum dots (QDs) are interesting alternatives to organic fluorophores for a range of applications such as biosensing, imaging and therapeutics. Addition of a programmable scaffold such as DNA to QDs further expands the scope and applicability of these hybrid nanomaterials in biology. In this review, the most important stages of preparation of QD-DNA conjugates for specific applications in biology are discussed. Special emphasis is laid on (i) the most successful strategies to disperse QDs in aqueous media, (ii) the range of different conjugation with detailed discussion about specific merits and demerits in each case, and (iii) typical applications of these conjugates in the context of biology.

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Section: Small Molecular Weight Ligandsmentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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“…The Stokes displacement (the D-value between the excitation peak and emission peak wavelengths) is so large (300-400 nm) that the overlap of the emission and excitation spectra can be avoided, thus allowing for spectroscopy detection even in cases of a low signal. QDs have a strong stable fluorescence intensity and are resistant to photobleaching (15)(16)(17)(18). The Qd, which is currently the focus of several studies, is a shell-core structure composed of a cdSe core and a ZnS shell with a diameter of 2-6 nm.…”

Section: Discussionmentioning

confidence: 99%

Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors

et al. 2011

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Abstract. The aim of this study was to evaluate the application of quantum dots (Qds) and the FiTc labeling technique in Tca8113 cells, and to compare the fluorescence intensity and photostability of these techniques. First, using one-colour confocal microscopy, Qds and FiTc were applied separately to tag HSP70 in Tca8113 strains with indirect immunofluorescence. The expression of HSP70 in Tca8113 cells was observed using confocal laser microscopy, and the fluorescence intensity between Qds and FiTc was compared. using two-colour confocal microscopy, Qds and FiTc were applied to tag survivin and HSP70, respectively, in order to compare the fluorescence photostability between QDs and FITC. Using a laser scanning microscope, survivin and HSP70 expression was clearly detected in the Tca8113 cells. The strength of the fluorescence signal from QDs was higher than that from FITC. after 40 min of continuous laser exposure, there was no observable decline in the QD fluorescent markers, whereas the FITC fluorescent signals faded very quickly and became undetectable. QDs have better fluorescence intensity and photostability than FiTc, and are more appropriate for studies that require a long dynamic observation of physiological changes in cells.

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“…These unique optical properties make QDs an excellent fluorescent probes for applications in the field of diagnosis (Bruchez et al, 1998;Chan and Nie, 1998), in vivo and in vitro imaging (Kim et al, 2003;Levene et al, 2004;Rosenthal et al, 2002), multicolor cell imaging (Hanaki et al, 2003;Jaiswal et al, 2002;Sukhanova et al, 2004;Wu et al, 2002), cell and protein tracking (Dahan et al, 2003;Voura et al, 2004), and DNA and protein sensing (Medintz et al, 2003;Zhang et al, 2005).…”

Section: Quantum Dotsmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker

Cited by 313 publications

References 22 publications

Quantum dots–DNA bioconjugates: synthesis to applications

Quantum dots–DNA bioconjugates: synthesis to applications

Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

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