Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors

Zhao, Jianjiang; Wang, Zhiping; Pan, Jie; Huang, Yuhua

doi:10.3892/mmr.2011.457

Cited by 11 publications

(10 citation statements)

References 21 publications

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“…Qdots are an attractive alternative to traditional fluorescent dyes for ICC because they are much brighter and more photostable [6–7]. In contrast to fluorescent dyes, Qdots can be excited with a wide range of wavelengths and have narrow emission spectra, which is advantageous for multiplex imaging [8–9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

Francis

Mason²,

Lévy

2017

Beilstein J. Nanotechnol.

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Semiconductor quantum dots (Qdots) have been utilised as probes in fluorescence microscopy and provide an alternative to fluorescent dyes and fluorescent proteins due to their brightness, photostability, and the possibility to excite different Qdots with a single wavelength. In spite of these attractive properties, their implemenation by biologists has been somewhat limited and only a few Qdot conjugates are commercially available for the labelling of cellular targets. Although many protocols have been reported for the specific labelling of proteins with Qdots, the majority of these relied on Qdot-conjugated antibodies synthesised specifically by the authors (and therefore not widely available), which limits the scope of applications and complicates replication. Here, the specificity of a commercially available, Qdot-conjugated secondary antibody (Qdot-Ab) was tested against several primary IgG antibodies. The antigens were labelled simultaneously with a fluorescent dye coupled to a secondary antibody (Dye-Ab) and the Qdot-Ab. Although, the Dye-Ab labelled all of the intended target proteins, the Qdot-Ab was found bound to only some of the protein targets in the cytosol and could not reach the nucleus, even after extensive cell permeabilisation.

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Section: Introductionmentioning

confidence: 99%

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

Francis

Mason²,

Lévy

2017

Beilstein J. Nanotechnol.

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“…In conclusion, our main goal was to develop a stable and active BMP-2 analog that could be visualized in a cellular context. Although most fluorophores rapidly degrade and are not photostable, QDot ® s can be used because of its photostability and intense fluorescence [9,10]. QDot ® s have also been used in previous studies to observe tumor progression and in vivo visualization [2,6].…”

Section: Discussionmentioning

confidence: 99%

“…For the first time, we report the stability and functionality of a BMP-2 analog. BMP-2 was conjugated to QDot ® s because QDot ® s are photostable, excitable, and limit photobleaching [ 7 , 9 ]. These qualities of QDot ® s are optimal to observe protein colocalization and movement [ 3 , 4 , 7 , 10 , 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Quantum Dots (QDot ® s) are novel, semi-conductive nanomaterials used for many biological applications such as tumor targeting, cellular environment imaging, and cancer metastasis tracking [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. Experimental usage of QDot ® s has surpassed traditional dyes such as green fluorescent proteins (GFPs) and fluorescein isothiocyanate (FITC) due to their superior photostability, brightness, and small particle size [ 8 , 9 , 10 ]. Furthermore, the outer shell of QDot ® s can be modified for its intended purpose; for example, this shell can be carboxylated to allow for coupling between QDot ® s and amine groups of proteins or peptides [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Bone Morphogenetic Protein-2 Conjugated to Quantum Dot®s is Biologically Functional

et al. 2020

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Quantum Dot®s (QDot®s) are novel, semi-conductive nanostructures that emit a certain fluorescence when excited by specific wavelengths. QDot®s are more photostable, brighter, and photobleach less than other fluorescent dyes. These characteristics give them the potential to be used in many biological applications. The shells of QDot®s are coated with functional groups, such as carboxylate and organic groups, allowing them to couple to peptides/proteins and be used for real-time imaging and high-resolution microscopy. Here, we utilize Quantum Dot®s and Bone Morphogenetic Protein-2 (BMP-2) to create a BMP-2-QDot®s conjugate. BMP-2 is a growth factor that drives many processes such as cardiogenesis, neural growth, and osteogenesis. Despite its numerous roles, the trafficking and uptake of BMP-2 into cells is not well-established, especially during progression of diseases. The results presented here demonstrate for the first time a fluorescent BMP-2 analog that binds to the BMP-receptors (BMPRs), remains biologically active, and is stable for long time periods. Previous attempts to develop a biological BMP-2 analog with Fluorescein isothiocyanate (FITC) or nanodiamonds lacked data on the analog’s stability. Furthermore, these analogs did not address whether they can signal within the cell by binding to the BMPRs or were mediated by non-stable conjugates.

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“…BsAbCENS (2 mg/ml) was labeled with fluorescein isothiocyanate (FITC; 1 mg/ml in dimethylsulphoxide) as described in a previous study (27). Prior to immunofluorescence staining, A549 and MRC-5 cells were cultured to 85% confluence.…”

Section: Treatments Of Patients With Lung Cancer Diagnosed Bymentioning

confidence: 99%

Contrast‑enhanced magnetic resonance imaging with a novel nano‑size contrast agent for the clinical diagnosis of patients with lung cancer

Gao

Liu

et al. 2018

Exp Ther Med

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Abstract. Recent studies have indicated that magnetic resonance imaging (MRI) efficiently diagnoses lung cancer. However, the efficacy of MRI in diagnosing lung cancer requires improving for patients in the early stage of the disease. In the present study, a novel nano-sized contrast agent of chistosan/Fe 3 O 4 -enclosed bispecific antibodies (BsAbCENS) was introduced, which targeted carcino-embryonic antigen (CEA) and neuron-specific enolase (NSE) in lung cancer cells. The diagnostic efficacy of contrast-enhanced MRI with BsAbCENS (CEMRI-BsAbCENS) was investigated in a total of 182 patients with suspected lung cancer who had high serum levels of CEA and NSE. BsAbCENS was administered by pulmonary inhalation prior to the MRI scan. The results revealed that CEA and NSE were overexpressed in human lung cancer cell lines. BsAbCENS bound with CEA and NSE on the surface of human lung cancer cells and produced a higher signal intensity than MRI alone for the diagnosis of patients with lung cancer. The diagnostic data revealed that CEMRI-BsAbCENS diagnosed 124/182 lung cancer cases, whereas CEMRI only diagnosed 98/182, which was significantly less (P<0.01). In addition, the survival rate of patients with lung cancer diagnosed by CEMRI-BsAbCENS was significantly higher than the mean 5-year survival rate (P<0.01). Furthermore, the pharmacodynamics demonstrated that BsAbCENS was metabolized within 24 h. The results of the present study indicate that the efficacy and accuracy of lung cancer diagnosis are improved by CEMRI-BsAbCENS. In conclusion, these results provide a potential novel protocol for the diagnosis of tumors in patients with suspected early stage lung cancer.

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Double labeling and comparison of fluorescence intensity and photostability between quantum dots and FITC in oral tumors

Cited by 11 publications

References 21 publications

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

Bone Morphogenetic Protein-2 Conjugated to Quantum Dot®s is Biologically Functional

Contrast‑enhanced magnetic resonance imaging with a novel nano‑size contrast agent for the clinical diagnosis of patients with lung cancer

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