Semiconducting Polymer Dots Doped with Europium Complexes Showing Ultranarrow Emission and Long Luminescence Lifetime for Time‐Gated Cellular Imaging

Sun, Wei; Yu, Jian; Deng, Ruiping; Rong, Yu; Fujimoto, Bryant S.; Wu, Changfeng; Zhang, Hongjie; Chiu, Daniel T.

doi:10.1002/anie.201304822

Cited by 93 publications

(58 citation statements)

References 31 publications

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“…With the development of fluorescence lifetime imaging microscopy and relevant techniques, optical multiplexing can employ nanomaterials with distinct fluorescence lifetime in a single color emission channel, which enables one‐color emissive nanomaterials to generate more populations and hence greatly extends its capability . Moreover, fluorescence lifetime lying in the microsecond region is superior for practical applications, because shorter lifetime on the order of nanoseconds cannot be easily separated from background noise by time‐gated technique, and longer lifetime on the order of milliseconds is less favorable for high‐throughput analysis . Therefore, it is highly desirable to prepare nanomaterials to simultaneously match all these four criteria for fluorescence‐lifetime optical multiplexing, including a single color emission with narrow band widths, tunable fluorescence lifetime in a wide time‐domain range, fluorescence lifetime on the order of microseconds, and high‐brightness luminescence.…”

Section: Introductionmentioning

confidence: 99%

Wide‐Range Tunable Fluorescence Lifetime and Ultrabright Luminescence of Eu‐Grafted Plasmonic Core–Shell Nanoparticles for Multiplexing

Zhang

Song

et al. 2015

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Wide-range, well-separated, and tunable lifetime nanocomposites with ultrabright fluorescence are highly desirable for applications in optical multiplexing such as multiplexed biological detection, data storage, and security printing. Here, a synthesis of tunable fluorescence lifetime nanocomposites is reported featuring europium chelate grafted onto the surface of plasmonic core-shell nanoparticles, and systematically investigated their optical performance. In a single red color emission channel, more than 12 distinct fluorescence lifetime populations with high fluorescence efficiency (up to 73%) are reported. The fluorescence lifetime of Eu-grafted core-shell nanoparticles exhibits a wider tunable range, possesses larger lifetime interval and is more sensitive to separation distance than that of ordinary Eu-doping core-shell type. These superior performances are attributed to the unique nanostructure of Eu-grafed type. In addition, these as-prepared nanocomposites are used for security printing to demonstrate optical multiplexing applications. The optical multiplexing experiments show an interesting pseudo-information "a rabbit in a well" and conceal the real message "NKU."

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Section: Introductionmentioning

confidence: 99%

Wide‐Range Tunable Fluorescence Lifetime and Ultrabright Luminescence of Eu‐Grafted Plasmonic Core–Shell Nanoparticles for Multiplexing

Zhang

Song

et al. 2015

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“…First, Pdots show low non-specific binding, which has been demonstrated in our previous work with flow cytometry. [18] Second, the size (typically 10 – 30 nm in diameter) of Pdots ensures that they will carry enough metal atoms for the purposes of mass cytometry. Third, the small size of metal-loaded Pdots will be completely converted into mass signals in the ICP torch, which will increase sensitivity.…”

mentioning

confidence: 99%

“…Furthermore, we can adjust the fluorescence color by using different Pdots synthesized with carboxyl-functional groups. [18b,19] Also, by changing the lanthanide salt, different Ln atoms can be coordinated into Pdots. Combining these two features, we potentially can create a series of dual-functionality probes for both flow and mass cytometry.…”

mentioning

confidence: 99%

Lanthanide‐Coordinated Semiconducting Polymer Dots Used for Flow Cytometry and Mass Cytometry

DeGottardi

et al. 2017

Angew Chem Int Ed

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Simultaneous monitoring of biomarkers as well as single-cell analyses based on flow cytometry and mass cytometry are important for investigations of disease mechanisms, drug discovery, and signaling-network studies. Flow cytometry and mass cytometry are complementary to each other; however, probes that can satisfy all the requirements for these two advanced technologies are limited. In this study, we report a probe of lanthanide-coordinated semiconducting polymer dots (Pdots), which possess fluorescence and mass signals. We demonstrated the usage of this dual-functionality probe for both flow cytometry and mass cytometry in a mimetic cell mixture and human peripheral blood mononuclear cells as model systems. The probes not only offer high fluorescence signal for use in flow cytometry, but also show better performance in mass cytometry than the commercially available counterparts.

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“…47 In comparison with semiconductor QDs, carbon-based fluorescent nanomaterials possess excellent biocompatibility, low cytotoxicity and chemical inertness, availability of multifunctional surface groups for covalent linkage of diverse bioactive molecules, making them promising candidates for imaging application. 64 These features have rendered them extremely useful for a number of applications including cellular imaging, targeted photodynamic therapy and in vivo tumor targeting. Nobel metal nanoclusters.…”

Section: Fluorescent Nanomaterialsmentioning

confidence: 99%

Nanomaterial-based activatable imaging probes: from design to biological applications

et al. 2015

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Activatable imaging probes as alternatives to "always on" imaging probes have attracted more and more attention due to their improved sensitivity and specificity. They are commonly designed to amplify or boost imaging signals only in response to specific biomolecular recognition or interaction. Thus, the design strategies play a vital role in the fabrication of activatable imaging probes. In this review, we focus on the design mechanisms and biological applications of those nanomaterial-based activatable imaging probes reported in the past five years, benefitting greatly from the good development of nanotechnology. These probes not only include the most studied activatable fluorescence imaging probes, but also cover more activatable MR imaging probes based on nanoparticle contrast agents and activatable photoacoustic imaging probes, providing more bases for clinical translation.

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Semiconducting Polymer Dots Doped with Europium Complexes Showing Ultranarrow Emission and Long Luminescence Lifetime for Time‐Gated Cellular Imaging

Cited by 93 publications

References 31 publications

Wide‐Range Tunable Fluorescence Lifetime and Ultrabright Luminescence of Eu‐Grafted Plasmonic Core–Shell Nanoparticles for Multiplexing

Wide‐Range Tunable Fluorescence Lifetime and Ultrabright Luminescence of Eu‐Grafted Plasmonic Core–Shell Nanoparticles for Multiplexing

Lanthanide‐Coordinated Semiconducting Polymer Dots Used for Flow Cytometry and Mass Cytometry

Nanomaterial-based activatable imaging probes: from design to biological applications

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