“…However like other bioassays, the microbial assay suffers from significant limitations; these methods are of lower specificity, accuracy and sensitivity. 3,6,7 Some efforts to combat the shortcomings of microbial assay have been reported so far, 8 for example Marengo and coworkers used a special test organism for microbiological assay of amikacin to improve the specificity of the method. They employed a strain of Providencia stuartii which is resistant to multiple antibiotics except amikacin.…”