Study of the Efficacy of Real Time-PCR Method for Amikacin Determination Using Microbial Assay

Lotfipour, Farzaneh; Yeganeh, Farshid; Tamizi, Elnaz; Zahedi, Amin; Asefi, Mohammadreza

doi:10.15171/apb.2015.025

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…It is also a complex method that involves expensive equipments, as well as very laborintensive procedures. 39 In another research Multiplex PCR method was applied by our team as an rapid method to control the microbial quality of pharmaceutical products. 40 The authors reported to develop a Multiplex PCR assay for simultaneous detection and identification of four indicator pathogenic bacteria of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella, in a single reaction.…”

Section: Novel Microbial Methods In Quality Controlmentioning

confidence: 99%

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

et al. 2016

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Section: Novel Microbial Methods In Quality Controlmentioning

confidence: 99%

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

et al. 2016

Self Cite

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“…Other methods are UV/Vis spectrophotometer [ 21 , 27 ], immuno-assay for the determination of AMK in serum using gliadins analyte model [ 23 , 26 ] are also reported. Chemiluminescence method using naturally occurring catalyzing agent [ 17 ] is also reported. Determination of AMK in human plasma by molecular imprinted SPR nano-sensor was also carried out by Isaksson et al [ 12 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

A rapid HPLC–DAD method for quantification of amikacin in pharmaceuticals and biological samples using pre-column derivatization with Hantzsch reagent

Maheshwari

Memon

et al. 2020

J IRAN CHEM SOC

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Amikacin (AMK) is an important member of aminoglycoside class, and its determination has therapeutic importance due to its matchless potency against gram –ve pathogens. Due to narrow therapeutic window, its monitoring in clinical samples is inevitable. Direct determination of AMK using HPLC with UV–visible detection is not possible because of its limited absorbance. Herein, Hantzsch reagent (mixture of acetylacetone, formaldehyde and acetate buffer) was used as pre-column derivatization for AMK. UV–visible detection was performed at 340 nm. Separation and identification of derivatized drug (amikacin) were carried out using C-18 column Kromasil 100 (15 cm × 0.46 mm, 5 μm) with isocratic mobile phase elution of pH 5 (acetate buffer 0.01 M):acetonitrile (30:70 v/v) with flow rate of 1 ml/min. The procedure was able to resolve AMK from endogenous compounds and from cephalosporin drug (most prescribed combination) with run time of 10 min. Under optimized conditions; calibration curve was linear in the range 0.10–25.0 µg/mL with LOD and LOQ values of 0.024 and 0.071 µg/mL. Method was also validated for reproducibility, ruggedness and accuracy. The procedure was found sensitive, robust and precise for the comprehensive analysis (qualitative and quantitative) that was applied for determination of AMK in pharmaceuticals, urine and blood samples.

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Kinetic microplate bioassays for relative potency of antibiotics improved by partial Least Square (PLS) regression

Francisco

Saviano

Botelho

et al. 2016

Journal of Microbiological Methods

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Study of the Efficacy of Real Time-PCR Method for Amikacin Determination Using Microbial Assay

Cited by 4 publications

References 19 publications

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

An Overview on Novel Microbial Determination Methods in Pharmaceutical and Food Quality Control

A rapid HPLC–DAD method for quantification of amikacin in pharmaceuticals and biological samples using pre-column derivatization with Hantzsch reagent

Kinetic microplate bioassays for relative potency of antibiotics improved by partial Least Square (PLS) regression

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