Semi-quantitative graphene chemiresistor enzyme immunoassay for simple and sensitive antigen detection

Miyakawa, Naruto; Shinagawa, Ayumi; Nakano, Tomomi; Ushiba, Shota; Ono, Takao; Kanai, Yasushi; Tani, Shinsuke; Kimura, Masahiko; Matsumoto, Kazuhiko

doi:10.1016/j.microc.2023.109594

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…Therefore, even minimal chemical dopants can substantially modulate the carrier (electron or hole) density of the graphene sheet. Solutiongated graphene FETs (SG-GFETs) have attracted considerable attention for detecting target molecules including ions, 5) gases, 6) proteins, 7,8) nucleic acids, 9,10) exosomes, 11,12) and viruses. 13,14) However, SG-GFETs exhibit a sensor drift or baseline drift.…”

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confidence: 99%

Drift suppression of solution-gated graphene field-effect transistors through electrolyte submersion

Ushiba,

Tokuda,

Nakano

et al. 2024

Appl. Phys. Express

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In solution-gated graphene field-effect transistors (SG-GFETs), cations in electrolyte solutions can intercalate between graphene and SiO2. Such permeation affects substrate-induced hole doping effects, resulting in drifts in the charge neutrality point (CNP) of SG-GFETs. In this study, we investigated the effect of submerging GFETs in electrolyte solutions on CNP values. The results revealed that the CNP decreased considerably from approximately 180 mV to nearly zero with the increase in the immersion period. The CNP drifts during electrical measurements were also suppressed by the prolonged submersion. These insights can be used for developing improved SG-GFETs.

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confidence: 99%

Drift suppression of solution-gated graphene field-effect transistors through electrolyte submersion

Ushiba,

Tokuda,

Nakano

et al. 2024

Appl. Phys. Express

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“…100 pM antigen was successfully detected in this experiment. 74 Shan et al designed a homogeneous fluorescence resonance energy transfer (FRET) based enzyme-linked immunosorbent assay (ELISA). Both donor and acceptor fluorescent microsphere-modified antibodies to Porcine Epidemic Diarrhea Virus (PEDV) can be associated solely with the presence of the PEDV antigen, resulting in FRET signals, which can be collected using a portable FRET Immunoassay Station (FRETIS) designed by us.…”

Section: Antigen Detectionmentioning

confidence: 99%

Advances in rapid point-of-care virus testing

Zhang,

Bu,

Shu

et al. 2024

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“…Particularly, graphene FETs (GFETs) are emerging as an exemplary sensing platform due to their unique twodimensional structure 3,4 and exceptional electron/hole mobility. 5,6 Existing research has shown GFET biosensors' efficacy in detecting a wide range of bio-analytes, including ions, 7,8 proteins, 9,10 small antigens, 11 exosomes, 12,13 and viruses. 14,15 Prior work has involved modifying GFETs with positively charged compounds.…”

Section: Introductionmentioning

confidence: 99%

Graphene Field-Effect Transistors with Surface-Charge Modulation for C-Reactive Protein Detection in Artificial Saliva

USHIBA,

NAKANO,

TOKUDA

et al. 2024

Electrochemistry

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Saliva-based biosensors are emerging as viable tools for noninvasive, painless, and easily administered household medical diagnostics. Despite their potential, the complexity of saliva, containing various non-target molecules and contaminants, presents significant challenges due to nonspecific interactions with biosensors. This study utilizes surface-charge modulated graphene field-effect transistors (SCM-GFETs) for the detection of C-reactive protein (CRP) in artificial saliva, using portable measurement apparatus. Our findings indicate that SCM-GFETs exhibit nonspecific responses to saliva components. To address this issue, a two-step preprocessing method is implemented: diluting the saliva in phosphate-buffered saline at a 1 : 10 4 ratio and subsequent membrane filtration. This process significantly reduced nonspecific interactions, enabling CRP detection in saliva samples. These advancements hold promise for enhancing graphene field-effect transistor technology in point-of-care diagnostic devices.

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Semi-quantitative graphene chemiresistor enzyme immunoassay for simple and sensitive antigen detection

Cited by 4 publications

References 44 publications

Drift suppression of solution-gated graphene field-effect transistors through electrolyte submersion

Drift suppression of solution-gated graphene field-effect transistors through electrolyte submersion

Advances in rapid point-of-care virus testing

Graphene Field-Effect Transistors with Surface-Charge Modulation for C-Reactive Protein Detection in Artificial Saliva

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