Semi-Quantitative and Qualitative Histologic Analysis Method for the Evaluation of Implant Biocompatibility

Jansen, John A.; Dhert, W.J.A.; Recum, Andreas F. von

doi:10.3109/08941939409015356

Cited by 91 publications

(90 citation statements)

References 10 publications

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“…Histological evaluation on all sections was performed using previously reported scoring methods [27]. Briefly, the parameters were fibrous capsule quality and fibrous capsule thickness, as a measure of cellular composition and maturity, and number of fibroblast layers surrounding the scaffolds, respectively ( Table 3).…”

Section: Histological and Histomorphometrical Analysismentioning

confidence: 99%

Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

et al. 2013

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Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs, Acta Biomaterialia (2012), doi: http://dx.doi.org/10. 1016/j.actbio.2012.10.026 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Histological and Histomorphometrical Analysismentioning

confidence: 99%

Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

et al. 2013

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“…Histological evaluation consisted of descriptive analysis and quantitative scoring of the connective tissue capsule surrounding the implants (Table I). 19 The Leitz light microscope was used for histological evaluation. Image analysis was performed with a computerized analysis system (Micro-Scale TC, Digithurst, Royston, UK).…”

Section: In Vivo Experimentsmentioning

confidence: 99%

Use of sol–gel‐derived titania coating for direct soft tissue attachment

Areva

Paldan

Peltola

et al. 2004

J Biomedical Materials Res

100

112

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A firm bond between an implant and the surrounding soft tissue is important for the performance of many medical devices (e.g., stents, canyls, and dental implants). In this study, the performance of nonresorbable and reactive sol-gel-derived nano-porous titania (TiO(2)) coatings in a soft tissue environment was investigated. A direct attachment between the soft tissue and the sol-gel-derived titania coatings was found in vivo after 2 days of implantation, whereas the titanium control implants showed no evidence of soft tissue attachment. The coated implants were in immediate contact with the connective tissue, whereas the titanium controls formed a gap and a fibrous capsule on the implant-tissue interface. The good soft tissue attachment of titania coatings may result from their ability to initiate calcium phosphate nucleation and growth on their surfaces (although the formation of poorly crystalline bonelike apatite does not occur). Thus, the formation of a bonelike CaP layer is not crucial for their integration in soft tissue. The formation of bonelike apatite was hindered by the adsorption of proteins onto the initially formed amorphous calcium phosphate growth centers, thus preventing the dissolution/reprecipitation processes required for the formation of poorly crystalline bonelike apatite. These findings might open novel application areas for sol-gel-derived titania-based coatings.

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“…22 The samples implanted subcutaneously were evaluated for the tissue response within the pores of the scaffold. The samples implanted into cranial defects were evaluated for the quality of the bone-implant interface as well as for the tissue response within the pores of the scaffold.…”

Section: Light Microscopy (Histological Scoring Analysis and Histomormentioning

confidence: 99%

Soft and hard tissue response to photocrosslinked poly(propylene fumarate) scaffolds in a rabbit model

Fisher

Vehof

Dean

et al. 2001

J. Biomed. Mater. Res.

241

153

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The treatment of large cranial defects may be greatly improved by the development of precisely formed bone tissue engineering scaffolds. Such scaffolds could be constructed by using UV laser stereolithography to photocrosslink a linear, biodegradable polymer into a three-dimensional implant. We have previously presented a method to photocrosslink the biodegradable polyester, poly(propylene fumarate) (PPF). To ensure the safety and effectiveness of this technique, the soft and hard tissue response to photocrosslinked PPF scaffolds of different pore morphologies was investigated. Four classes of photocrosslinked PPF scaffolds, constructed with differing porosities (57-75%) and pore sizes (300-500 or 600-800 microm), were implanted both subcutaneously and in 6.3-mm-diameter cranial defects in a rabbit model. The rabbits were sacrificed at 2 and 8 weeks, and the implants were analyzed by light microscopy, histological scoring analysis, and histomorphometric analysis. Results showed the PPF scaffolds elicit a mild tissue response in both soft and hard tissues. Inflammatory cells, vascularization, and connective tissue were observed at 2 weeks; a decrease in inflammatory cell density and a more organized connective tissue were observed at 8 weeks. Scaffold porosity and scaffold pore size were not found to significantly affect the observed tissue response. Evidence of scaffold surface degradation was noted both by histology and histomorphometric analysis. Bone ingrowth in PPF scaffolds implanted into cranial defects was <3% of the defect area. The results indicate that photocrosslinked PPF scaffolds are biocompatible in both soft and hard tissues and thus may be an attractive platform for bone tissue engineering.

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Semi-Quantitative and Qualitative Histologic Analysis Method for the Evaluation of Implant Biocompatibility

Cited by 91 publications

References 10 publications

Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

Use of sol–gel‐derived titania coating for direct soft tissue attachment

Soft and hard tissue response to photocrosslinked poly(propylene fumarate) scaffolds in a rabbit model

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