Semi-automated high-performance liquid chromatographic method for the determination of cyclosporine in plasma and blood using column switching

Smith, Hamilton; Robinson, William T.

doi:10.1016/s0378-4347(00)83349-6

Cited by 42 publications

(1 citation statement)

References 13 publications

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“…method of Smith & Robinson (1984) wZs adapted as follows: to 0.5 ml whole blood, 0.1 ml methanol was added and vortexed for 5 s. Then 1.2 ml of internal standard solution (250 ng/ml of Cyclosporin D in acetonitrile/water 97.5/2.5 v/v) were added, the tubes vortexed again for 30 s and then centrifuged for 10 min at 1,400 rev/min. The clear supernatant Ir was decanted into a conical glass tube to which 1 ml N-hexane was added.…”

Section: Protocolmentioning

confidence: 99%

Pharmacokinetic study of cyclosporin A (Sandimmun) in patients with primary biliary cirrhosis.

Robson¹,

Neuberger²,

Keller³

et al. 1984

Brit J Clinical Pharma

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The pharmacokinetics of cyclosporin A (CS‐A) were studied in 10 patients with primary biliary cirrhosis (PBC) after oral administration in steady state. Mean values for area under the blood concentration‐ time curve (AUC), time to maximal blood concentration (tmax), maximal blood concentration (Cmax) and elimination half‐life (t1/2,z) were similar to results of previous studies in transplant patients. The variation between patients was large. No significant correlations of pharmacokinetic data with biochemical or histological parameters were found. Because of the high variability of pharmacokinetic parameters, patients with PBC treated with CS‐A need to be regularly controlled for nephrotoxicity by estimation of serum creatinine and bioavailability (trough blood levels).

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Section: Protocolmentioning

confidence: 99%

Pharmacokinetic study of cyclosporin A (Sandimmun) in patients with primary biliary cirrhosis.

Robson¹,

Neuberger²,

Keller³

et al. 1984

Brit J Clinical Pharma

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Cyclosporine Concentration Determinations for Monitoring and Pharmacokinetic Studies

Ptachcinski

Burckart

Venkataramanan

1986

The Journal of Clinical Pharma

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The availability of the immunosuppressant cyclosporin has led to significant improvements in the recent success of clinical organ transplantation. Problems associated with cyclosporine therapy include serious adverse reactions, such as nephrotoxicity, wide variability in the drug's pharmacokinetics, and several complex drug interactions. Monitoring of drug concentrations is accepted as a part of the routine care of patients receiving cyclosporine. However, cyclosporine concentrations can be determined in different biologic fluids by either radioimmunoassay or high-performance liquid chromatographic techniques. Controversy exists regarding the optimal analytic technique to be used for cyclosporine monitoring and pharmacokinetic studies. This commentary addresses factors including: why the monitoring of cyclosporine concentrations is important, the differences between the biologic fluids and analytic techniques, when monitoring and special pharmacokinetic studies are indicated, what some major transplant centers have established as a "therapeutic range" for cyclosporine concentrations, and provides guidelines for the optimal clinical monitoring of cyclosporine concentrations.

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Cyclosporine Blood Concentrations Determined by Specific Versus Nonspecific Assay Methods

Blyden

Franklin

Cho

et al. 1986

The Journal of Clinical Pharma

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Cyclosporine blood concentrations were simultaneously determined by radioimmunoassay (RIA) and high-performance liquid chromatography (HPLC) at multiple points in time in two patients receiving cyclosporine for immunosuppression following liver transplantation. Radioimmunoassay levels always exceed those determined by HPLC; however, the divergence between the two methods increased as serum bilirubin concentration increased, with HPLC:RIA ratios generally less than 0.3 when serum bilirubin concentrations exceeded 10.0 mg/dL. These preliminary results suggest that retention of immunoactive cyclosporine metabolites due to imparied liver function may account for RIA-determined cyclosporine concentrations that greatly exceed those measured by HPLC.

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Semi-automated high-performance liquid chromatographic method for the determination of cyclosporine in plasma and blood using column switching

Cited by 42 publications

References 13 publications

Pharmacokinetic study of cyclosporin A (Sandimmun) in patients with primary biliary cirrhosis.

Pharmacokinetic study of cyclosporin A (Sandimmun) in patients with primary biliary cirrhosis.

Cyclosporine Concentration Determinations for Monitoring and Pharmacokinetic Studies

Cyclosporine Blood Concentrations Determined by Specific Versus Nonspecific Assay Methods

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