Semen Traits and Seminal Plasma Biochemical Parameters in White Leghorn Layer Breeders

Abstract

This study was designed to determine the semen quality and seminal plasma biochemical parameters in White Leghorn layer breeders in the early phase of maturity. Individual ejaculates from 25 males were analysed for the determination of volume, sperm concentration, dead sperm percentage (DS) and sperm motility. Seminal plasma was separated and analysed for total oxidant status (TOS), total antioxidant capacity (TAC), paraoxonase (PON1), arylesterase, ceruloplasmin, triiodothyronine, thyroxine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Our findings showed a significant (p < 0.05) positive correlation between TOS and DS in layer breeders. The values of TAC were significantly negatively correlated with TOS and DS, while positively correlated with PON1. Conversely, AST showed significant negative correlation with motility and DS. Significantly, negative correlation was also observed between ALT and sperm concentration. In conclusion, these parameters provide some valuable basal data that may help better understanding the semen quality characteristics of White Leghorn layer breeders.

Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

“…Boar seminal plasma concentrations of PON‐1 indeed vary among individuals, although the overall range is low – similar to that reported for humans (Verit et al, ; Marsillach et al, ). More surprising is the variation in PON‐1 enzymatic activity among ejaculates, which is again consistent with reports for humans (Verit et al, ; Marsillach et al, ) and white leghorn layer breeders (Khan et al, ). Such divergence between PON‐1 enzyme concentration versus activity also appears in blood serum levels of obese compared to non‐obese children (Ferre et al, ), pointing to a common regulatory mechanism.…”

“…PON enzymes have also been detected in a variety of tissues, including mouse testis, specifically in spermatogonia, spermatocytes, and round and elongated spermatids (Marsillach et al, ). Additionally, PON‐1 has been identified in human seminal‐plasma (Verit et al, ; Marsillach et al, ), in bull ejaculate (Dedeoğlu et al, ), and in the seminal plasma of white leghorn layer roosters (Khan et al, ). In human seminal plasma, PON‐1 levels are relatively low (Marsillach et al, ), but its quantities are related to oxidative stress in semen and infertility (Verit et al, ; Lazaros et al, ).…”

Effect of Testosterone Administration and Spiking on Reproductive Success of Broiler Breeder Flocks

“…; Gumulka and Kapkowska ; Khan et al. ), sexual behaviour (Mauldin ), body weight and muscular‐skeletal lesions (Hocking and Duff ). Given the multiple factors involved and the interactions between them, it is difficult to establish the relative importance of individual factors on the decline of reproductive fitness.…”

“…Ageing has important implications in the management of broiler breeder flocks at later stages of flock life because age negatively affects reproductive success or fitness of birds. Multiple factors are involved in the decrease of reproductive fitness with age: hormonal level (Weil et al 1999;Avital-Cohen et al 2013), qualitative parameters of semen (Bramwell et al 1996;Gumulka and Kapkowska 2005;Khan et al 2012), sexual behaviour (Mauldin 1992), body weight and muscularskeletal lesions (Hocking and Duff 1989). Given the multiple factors involved and the interactions between them, it is difficult to establish the relative importance of individual factors on the decline of reproductive fitness.…”

Effects of dietary vitamin E on male reproductive system

“…This enzyme has the ability to convert the superoxide anion into hydrogen peroxide on both side of cell as well as the conversion of this hydrogen peroxide into water. Reduced quantity of SOD results into poor quality of semen as well as reproductive functions [50]. Glutathion peroxidase (GPx) is considered as the important antioxidant and reduces the amount of lipid peroxidation.…”