Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Barranco, Isabel; Roca, Jordi; Tvarijonaviciute, Asta; Rubér, Marie; Vicente-Carrillo, Alejandro; Atikuzzaman, Mohammad; Cerón, José J.; Martı́nez, Emilio A.; Rodriguez‐Martinez, Heriberto

doi:10.1002/mrd.22444

Cited by 24 publications

(25 citation statements)

References 39 publications

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“…Therefore, the spermatozoa benefit from the presence of antioxidant components in the seminal plasma (SP) in which they are bathed (Juyena & Stelletta, ). The boar SP contains antioxidant enzymes (Strzezek, ), including paraoxonase 1 (PON‐1) (Barranco et al ., ); an extracellular high‐density lipoprotein (HDL)‐associated enzyme that possess antioxidant and anti‐inflammatory properties, which prevents low‐density lipoprotein and HDL oxidation and, consequently, protects cells against OS (Camps et al ., ). In humans, lowered SP‐PON‐1 levels have been related to increased OS and infertility (Verit et al ., ; Lazaros et al ., ), and SP‐PON‐1 activity has been positively correlated with sperm parameters, such as concentration, motility, and morphology (Verit et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

et al. 2015

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SUMMARYParaoxonase 1 (PON-1) is a hydrolytic enzyme present in body fluids, capable of protecting cells against oxidative stress. The hypothesis was hereby to test that PON-1, present in seminal plasma (SP), acts protecting boar spermatozoa when showing a reasonable high activity in the ejaculate. SP-PON-1 activity differed (p < 0.001) among boars (from 0.10 to 0.29 IU/mL). Intra-boar variability was also observed (p < 0.05), but only in two of the 15 boars. SP-PON-1 activity differed among ejaculate portions, showing the spermatozoa-peak portion of spermatozoa-rich ejaculate fraction the highest levels (0.35 AE 0.03 IU/mL, ranging from 0.12 to 0.69) and the post-sperm ejaculate fraction the lowest levels (0.12 AE 0.01 IU/mL, ranging from 0.03 to 0.21). SP-PON-1 activity was positively correlated with the percentage of spermatozoa with rapid and progressive movement (p < 0.01) and negatively correlated with the generation of intracellular reactive oxygen species (p < 0.01) in semen samples after 72 h of liquid storage. SP-PON-1 activity was highest (p < 0.01) in boars with highest farrowing rates. In conclusion, SP-PON-1 activity differed among boars and ejaculate fractions/portions. SP-PON-1 activity was positively correlated with sperm quality and functionality of liquid-stored semen samples and it evidenced a positive association with in vivo fertility.

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Section: Introductionmentioning

confidence: 99%

The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

et al. 2015

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“…Sperm cell structure and function depend on the lipid composition in sperm cells and SP (Barranco et al, 2015;Wood et al, 2016) which in turn affect its fertilisation capacity. Cholesterol is a key factor in semen quality and function where analysis of semen samples from stallions with unexplained subfertility revealed a low concentration of SP cholesterol (Brinsko et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Protein and lipid species in seminal plasma of fertile Holstein-Friesian bulls

Almadaly¹,

Farrag²,

El-Nasr³

2019

BJVM

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Protein and lipid molecules in seminal plasma (SP) collected from fertile bulls were investigated. Semen was collected from 10 bulls (2 ejaculates each) and examined for standard semen analysis. Raw SP was recovered by centrifugation and total protein (TP) concentration was determined using a refractometer. Raw SP was desalted using a Sephadex G-25 desalting column then both raw and desalted SP was subjected to SDS-PAGE. Neutral lipids and phospholipids of raw and desalted SP were separated by thin-layer chromatography (TLC). The results revealed that, all bulls had normal semen characteristics and TP concentration in SP ranged from 7.0 to 10.4 g/dL except bull No. 6 had a relatively low concentration of 4.9 to 6.8 g/dL. Neither proteins nor lipids species were different between raw and desalted SP. Seventeen proteins were detected ranging from 8.5 to 185.8 kDa, and those of 12, 13.5, 15, 21, 23 and 38 kDa were predominant. Notably, proteins of 10, 17.5, 19, 21, 80 and 185.8 kDa might be new candidates of SP proteins (SPPs). The detected neutral lipid spots corresponded to cholesterol, 1,2-dimyristoyl glycerol, 1,2-dioleoyloglycerol, 1,3-dimyristoyl glycerol and 1,3-dioleoyloglycerol. The detected phospholipids spots corresponded to non-migrating phospholipids, sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylethanolamine (PE), cerebroside and polyglycerol phosphatide. Cholesterol represents the major molecule of neutral lipids, whereas SM, PC, PI and PE represent the major phospholipids. Noteworthy, there were 2 species of diacylglycerol (DAG) and 3 species of PI in bovine SP. In conclusion, this study gave a general picture of SP protein and lipid species in fertile bull semen, which might serve as fundamental knowledge for either semen analysis or prediction of male fertility.

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“…Barranco et al [97] revealed and characterized the presence of the antioxidant enzymes PON-1 and PON-2 in boar semen. PON-1 binds to membrane cholesterol, preventing its oxidation and thereby positively influencing both motility and the sperm membrane integrity [46].…”

Section: Paraoxonase Typementioning

confidence: 99%

Relationship between Plasma Proteins and Boar Semen Freezability

Henao¹

2019

Animal Genetics - Approaches and Limitations

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Currently, there is great interest in using frozen boar semen to enhance pig-breeding processes. Yet semen freezability, as well as its limited lifespan in the uterus, limits the efficacy of such a procedure. Pig spermatozoa membrane is less stable and more sensitive to low temperatures as it contains lower levels of cholesterol. It is also highly susceptible to lipid peroxidation (LPO) during freezing, since it is rich in polyunsaturated fatty acids (PUFA). Seminal plasma (SP) has beneficial effects on post-thaw semen quality and its composition may have a genetic basis, specifically in protein content. To date, studies on boar semen freezability have focused on sperm cell proteins with very little attention having been paid to SP proteins. In boar SP, there are 82 identified proteins with spermadhesins (90%) and fibronectins (FN) the most abundant. The only plasma protein thus far identified as a freezability marker is FN1. Other plasmatic proteins of recognized importance in the freezing of porcine semen are: DQH, HSP90AA1, NPC2, L-PGDS, ß-HEX, SOD, and PON-1. The purpose of this chapter is to examine the most efficacious elements of the above plasma proteins with regard to their role as biological or potential biological markers of porcine semen freezability.

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Measurement of activity and concentration of paraoxonase 1 (PON‐1) in seminal plasma and identification of PON‐2 in the sperm of boar ejaculates

Cited by 24 publications

References 39 publications

The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

The activity of paraoxonase type 1 (PON‐1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility

Protein and lipid species in seminal plasma of fertile Holstein-Friesian bulls

Relationship between Plasma Proteins and Boar Semen Freezability

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