Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Eslami, Mohsen; Ghasemiyan, Hossein; Hashem, Elham Zadeh

doi:10.1111/rda.12802

Cited by 17 publications

(22 citation statements)

References 43 publications

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“…The efficiency of TL-HEPES and PBS in sperm processing (16,17) and in short-term storage (18)(19)(20) was identified in previous studies. Tris-citrate is a commonly used extender (1), and it is also usually used as the control or main extender in ram sperm-extender studies (21)(22)(23)(24). TEST, TL-HEPES, and PBS extenders were found to be effective in protecting the sperm against cold shock when freezing rat sperm and in equilibration at 4 °C (20,25).…”

Section: Introductionmentioning

confidence: 99%

Effects of different extenders and additives on liquid storage of Awassi ram semen

Varışlı¹,

Taşkın²,

Akyol³

2018

Turk J Vet Anim Sci

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The breeding season is an important part of sheep breeding since it allows early lambing. Regarding the Awassi raised in warm climate zones, lambs born early in the year can develop more quickly as they will not be affected by the hot season, which has more stress factors than the cold and rainy season. There are approximately 30 million sheep in Turkey, and most of them are of domestic races. The Awassi race is found in the Southeastern Anatolia Region, and they number around 4 million (5-7). Awassi sheep are one of the races adapted to the steppe conditions in southeastern Turkey. These are the most commonly bred sheep in the region. In state farms, milk production is 300 to 350 kg per lactation, whereas in conventional farms, it is only 100 to 150 kg per lactation. In sheep breeding, due to the large scale of the herd to be inseminated and breeding performed over long distances, sperm must be transported without any problems and loss in its fertility capacity. Moreover, to take advantage of the rams for longer periods and in various times of the year, sperm storage technologies should be more advanced. The basic principle of sperm storage is to reduce the spermatozoa metabolism, thus

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Section: Introductionmentioning

confidence: 99%

Effects of different extenders and additives on liquid storage of Awassi ram semen

Varışlı¹,

Taşkın²,

Akyol³

2018

Turk J Vet Anim Sci

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“…Reports have shown that ram semen enrichment with butylated hydroxytoulene, taurine, catalase, quercetin, palmitoleic acid, and kinetin enhances motility or viability compared to the control when stored as liquid at refrigerator or after freeze‐thawing process (Banday et al, ; Câmara et al, ; Eslami et al, ; Rather et al, ; Zadeh Hashem & Eslmi, ). The results of the present experiment indicated that Id at 1, 2 and 4 µM enhanced total motility, FPM, and VCL at 48 and 72 hr of storage.…”

Section: Discussionmentioning

confidence: 99%

“…Reports have shown that ram semen enrichment with butylated hydroxytoulene, taurine, catalase, quercetin, palmitoleic acid, and kinetin enhances motility or viability compared to the control when stored as liquid at refrigerator or after freeze-thawing process (Banday et al, 2017;Câmara et al, 2011;Eslami et al, 2017;Rather et al, 2016;Zadeh Hashem & Eslmi, 2018 experiment. Previous study revealed a correlation between capacities of spermatozoa in fertilization with spermatozoa motility assessed by CASA (Larsen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…However, spermatozoa of mammals do not have a significant cytoplasmic component to support sufficient antioxidants against the detrimental effects of ROS and LPO (Lapointe & Bilodeau, ). During liquid storage of semen, LPO occurs and increases in a time dependency manner (Eslami, Ghasemiyan, & Zadeh, ; Zadeh Hashem, Haddad, & Eslami, ). Studies showed that substances that have anti‐oxidative properties may be useful to prevent or decrease the detrimental effects of LPO during semen preservation technique (Banday, Lone, Rasool, Rashid, & Shikari, ; Bucak et al, ; Rateb, ; Rather, Islam, Malik, & Lone, ; Sarıözkan, Bucak, Tuncer, Ulutas, & Bilgen, ).…”

Section: Introductionmentioning

confidence: 99%

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Influence of idebenone on ram semen quality stored at 4°C

Eslami

Jahan-Roshan

Farrokhi‐Ardabili

2018

Reprod Domestic Animals

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Contents The current study was designed to investigate the effect of idebenone (Id), an antioxidant on ram semen quality. Semen samples were collected, pooled and diluted in a Tris‐based extender supplemented with 0, 1, 2, 4 and 8 µM idebenone. Computer‐assisted sperm analysis was used to evaluate spermatozoa kinematics. Sperm viability and membrane functionality were assessed respectively, by eosin‐nigrosin staining and HOS test. Biochemical assays were carried out to measure different metabolites in spermatozoa and medium at 0, 24, 48 and 72 hr. Total and forward progressive motility were greater in 1, 2 and 4 µM idebenone treated groups compared to control at 24, 48 and 72 hr time points (p < 0.05). Semen supplementation with Id significantly increased viability and functionality of spermatozoa membrane during storage (p < 0.05). Lower amounts of lipid hydroperoxides in medium and spermatozoa were observed in Id‐treated groups compared to control one at 24 and 48 hr of storage (p < 0.05). Medium and spermatozoa amounts of malondialdehyde and nitric oxide were less in Id 4 µM group compared to the control at 72 hr (p < 0.05). Total antioxidant capacity values and superoxide dismutase activity of spermatozoa and medium were greater in 2 and 4 µM idebenone treated groups in comparison with the control at 72 hr (p < 0.05). Results of the current study indicated that ram semen supplementation with Id at 4 µM level improved quality by ameliorating nitrosative and peroxidative stress, hence could be considered as an antioxidant additive during storage at 4°C.

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“…Although we have not yet had the opportunity to test this hypothesis directly in crocodile spermatozoa, we did secure several lines of correlative evidence that these cells use a similar metabolic strategy. Thus, capacitated crocodile spermatozoa experienced a selective depletion of palmitoleic acid [a monounsaturated fatty acid substrate known to enhance the motility profile of spermatozoa from species as diverse as sheep and fowls (35,36)], as well as a significant reduction in motility following the uncoupling of oxidative phosphorylation. Moreover, we identified CPT1A as a substrate for differential phosphorylation in noncapacitated versus capacitated crocodile spermatozoa.…”

Section: Molecular and Cellular Proteomics 1813 S73mentioning

confidence: 99%

Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals*

Nixon¹,

Johnston²,

Skerrett‐Byrne³

et al. 2019

Molecular & Cellular Proteomics

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Competition to achieve paternity has contributed to the development of a multitude of elaborate male reproductive strategies. In one of the most well-studied examples, the spermatozoa of all mammalian species must undergo a series of physiological changes, termed capacitation, in the female reproductive tract prior to realizing their potential to fertilize an ovum. However, the evolutionary origin and adaptive advantage afforded by capacitation remains obscure. Here, we report the use of comparative and quantitative proteomics to explore the biological significance of capacitation in an ancient reptilian species, the Australian saltwater crocodile (Crocodylus porosus). Our data reveal that exposure of crocodile spermatozoa to capacitation stimuli elicits a cascade of physiological responses that are analogous to those implicated in the functional activation of their mammalian counterparts. Indeed, among a total of 1119 proteins identified in this study, we detected 126 that were differentially phosphorylated (± ≥1.2 fold-change) in capacitated versus non-capacitated crocodile spermatozoa. Notably, this subset of phosphorylated proteins shared substantial evolutionary overlap with those documented in mammalian spermatozoa, and included key elements of signal transduction, metabolic and cellular remodeling pathways. Unlike mammalian sperm, however, we noted a distinct bias for differential phosphorylation of serine (as opposed to tyrosine) residues, with this amino acid featuring as the target for ~80% of all changes detected in capacitated spermatozoa. Overall, these results indicate that the phenomenon of sperm capacitation is unlikely to be restricted to mammals and provide a framework for understanding the molecular changes in sperm physiology necessary for fertilization.

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Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Cited by 17 publications

References 43 publications

Effects of different extenders and additives on liquid storage of Awassi ram semen

Effects of different extenders and additives on liquid storage of Awassi ram semen

Influence of idebenone on ram semen quality stored at 4°C

Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals*

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