Semen of Perca fluviatilis L.: Sperm volume and density, seminal plasma indices and effects of dilution ratio, ions and osmolality on sperm motility

Alavi, Sayyed Mohammad Hadi; Rodina, Marek; Policar, T.; Kozák, Pavel; Pšenička, Martin; Linhart, Otomar

doi:10.1016/j.theriogenology.2007.05.045

Cited by 82 publications

(84 citation statements)

References 34 publications

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“…It is noteworthy that calcium concentrations were markedly higher on the first 2 sampling days in river water (rain event), while potassium concentrations varied little across all 5 river water collection days. Considering the enhancing effects of potassium on sperm motility (Tan-Fermin et al 1999;however, Alavi et al (2007) did not report this enhancing effect) and the inhibiting effect of higher calcium concentrations (through increased osmolality) on sperm motility (Alavi et al 2007), it appears that this field observation is consistent with published laboratory studies in finding diminished motility of goldfish sperm activated in river water immediately after the rain event (high calcium) compared to activations several days later (lower calcium concentrations). Although it was beyond the scope of this study to determine which components of river water were responsible for the observed change in sperm performance, the natural variability of river water must be taken into account if such environments are used as reference sites for studies of effluent effects.…”

Section: Discussionsupporting

confidence: 64%

“…In addition, we prepared a synthetic ion mixture to simulate the osmolality of the effluent (measured previously) while excluding the overwhelming majority of potentially toxic compounds in the treated wastewater effluent. The synthetic ion mixture was based only on the ions of calcium, potassium, chloride, and sodium, which are documented to be central to sperm activation and motility (Alavi et al 2007). All aliquots (*30 aliquots for each treatment per analysis day) were stored at 4°C until being used for sperm activation (within 3 h of dilution).…”

Section: Treatmentsmentioning

confidence: 99%

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Treated Wastewater Effluent Reduces Sperm Motility Along an Osmolality Gradient

Schoenfuss

Levitt²,

Rai

et al. 2008

Arch Environ Contam Toxicol

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Many toxic effects of treated wastewater effluent on organismal and reproductive health have been documented. However, the physicochemical environment of treated wastewater effluent frequently differs considerably from that of its receiving waters and may affect organismal function independently of toxic effects. Teleost sperm, for example, may be affected by the higher osmolality of treated wastewater, as this sperm is activated for a brief period of time following ejaculation due to the sudden decrease in osmolality of its surrounding environment. In this study, we examined the effects of treated wastewater effluent on sperm motility to test the hypothesis that the higher osmolality of effluent compared to river water will adversely affect sperm activation in a concentrationdependent relationship. Treated wastewater effluent was collected on 5 days from the outflow of the Metropolitan Wastewater Treatment Plant, St. Paul, Minnesota, and from an upstream site on the Mississippi River. Milt aliquots collected from goldfish were diluted in an isotonic extender solution and subsequently activated in either deionized water, 100%, 50%, or 10% effluent, a synthetic ion mixture, or river water. Sperm motility and velocity were assessed at 15-s intervals for 1 min using a computer assisted sperm analyzer. Significant differences in performance parameters were found only at 15 s, with sperm motility and velocity declining rapidly at later sampling times. Predictably, deionized water resulted in the greatest activation of sperm motility, while motility exhibited a concentration-dependent decline in 10%, 50%, and 100% treated wastewater effluent. Interestingly, Mississippi River water and a synthetic ion mixture with an osmolality comparable to 50% effluent both resulted in the least amount of sperm activation. However, sperm activation in river water varied between collection days during the study. River water and 100% effluent both had low sperm activation characteristics despite a 10-fold difference in osmolality between these two treatments (1 and 10 mOsmol kg -1 , respectively). Results of this study indicate a concentration-dependent decrease in sperm motility in treated wastewater effluent as well as significant fluctuations of sperm activation in Mississippi River water. This study illustrates the complexity of assessing the effects of treated wastewater effluents and the difficulty of determining appropriate reference sites for such studies.Many effects of treated wastewater effluent on the reproductive ability of fish have been examined. However, the ability of freshwater teleost sperm to become motile after ejaculation, a crucial link to the reproductive success in a mating event, has received less attention (Kime 1999;Kime and Nash 1999). While research has more often focused on semen and sperm quality of exposed male fish (Schoenfuss et al. 2002;Lahnsteiner et al. 2005a, b;Runnalls et al.

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Section: Discussionsupporting

confidence: 64%

Section: Treatmentsmentioning

confidence: 99%

Treated Wastewater Effluent Reduces Sperm Motility Along an Osmolality Gradient

Schoenfuss

Levitt²,

Rai

et al. 2008

Arch Environ Contam Toxicol

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“…O volume de sêmen observado no dourado (Tabela 1), assim como em outras espécies de peixes brasileiras, apresentou diferenças interespecíficas (Andrade-Talmelli et al, 2001;Ninhaus-Silveira et al, 2006;Bombardelli et al, 2006a), no entanto, essas variações podem ocorrer de acordo com o método de colheita, uma vez que a extrusão não garante a liberação total do sêmen presente nas gônadas ).…”

Section: Resultsunclassified

“…Associadas ao processo de diluição do meio inseminante, a motilidade espermática pode ser afetada pela composição da solução ativadora e do plasma seminal (Emri et al, 1998). As principais variáveis afetadas neste caso podem ser a viscosidade (Billard & Cosson, 1992), o pH (Alavi & Cosson, 2005), a concentração dos íons K + , Na + , Ca 2+ , Mg 2+ (Alavi & Cosson, 2006), a osmolaridade (Alavi et al, 2007), a atividade proteica (Fauvel et al, 1999), os níveis de lipídeos totais, as atividades enzimáticas e os níveis de ATP e NADH espermáticas (Lahnsteiner et al, 1998). Esses fatores, correlacionados corretamente, aumentam a duração da motilidade espermática e consequentemente a capacidade de fertilização (Cosson, 2004).…”

Section: Resultsunclassified

Dose inseminante para fertilização artificial de ovócitos de dourado

et al. 2009

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RESUMO -Objetivou-se determinar a dose inseminante adequada para uso na fertilização artificial de ovócitos de dourado (Salminus brasiliensis). Os ovócitos foram distribuídos em delineamento inteiramente casualizado, e fertilizados com uma das relações espermatozoides/ovócito 6,0×10 3 ; 6,0×10 4 ; 6,0×10 5 ; 6,0×10 6 ou 3,0×10 7 , cada uma com quatro repetições.Considerou-se unidade experimental uma incubadora de volume útil de 2,5 L, contendo 2,0 mL de ovócitos não-hidratados.As taxas de fertilização foram mensuradas 8 horas após o início da fertilização. Com intuito de verificar possíveis efeitos da diluição seminal na movimentação dos espermatozoides, realizou-se a mensuração do tempo de duração da motilidade espermática dos espermatozoides de dourado, ativados por meio de diferentes relações de diluição: 6,8×10 -5 ; 6,8×10 -4 ; 6,8×10 -3 ; 6,8×10 -2 ; 3,4×10 -1 e 1,0 mL de sêmen por mL de água. O tempo de duração da motilidade foi avaliado em delineamento inteiramente casualizado composto de seis tratamentos e três repetições. As taxas de fertilização apresentaram relação quadrática com o número de espermatozoides por ovócito. As relações de diluição do sêmen tiveram efeito inversamente proporcional sobre a duração da motilidade espermática. A relação que proporcionou melhores taxas de fertilização artificial Insemination dose for artificial fertilization of dourado oocytesABSTRACT -The objective of the present study was to determine the proper insemination dose of dourado (Salminus brasiliensis) oocytes. The oocytes were placed in a randomized complete design and fertilized with one of the spermatozoa.oocytes -1 ratio, 6.0×10 3 , 6.0×10 4 , 6.0×10 5 , 6.0×10 6 , 3.0×10 7 SPZ:OOC, each one with four replications. An experimental unit was considered to be an incubator with a 2.5L useful volume containing 2.0 mL non-hydrated oocytes. The fertilization rates were measured eight hours after the start of fertilization. In order to ascertain the possible effects of seminal dilution on the spermatozoa motility, the duration time of the spermatic motility of the dourado spermatozoa was measured when activated by different dilution ratios 6.8×10 -5 ; 6.8×10 -4 ; 6.8×10 -3 ; 6.8×10 -2 ; 3.4×10 -1 and 1.0 mL semen.mL water -1 . The motility duration time was assessed in a randomized complete design, with six treatments and three repetitions. The fertilization rates showed a quadratic relationship with the number of spermatozoids per oocyte. The semen dilution ratios had an inversely proportional affect on the spermatic motility time. The spermatozoa:oocytes ratio that provided the best artificial fertilization rates of dourado (Salminus brasiliensis) oocytes was 30,722 spermatoids per oocyte.

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“…In addition, the percentage of sperm with progressive motility was the highest after activation with AS2 buffer (16%). Decreasing osmotic pressure affects the motility of fish sperm (Alavi et al, 2007). In cyprinids, excessively high osmotic pressure, above the values typical of seminal plasma, 170 to 204 mOsmkg -1 for ide (Cejko et al, 2010a); 252 for orfe (this paper), has an inhibiting effect on sperm motility (Billard et al, 1995).…”

Section: Optimum Buffer Selectingmentioning

confidence: 76%

Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

Sarosiek

Cejko

Targońska

et al. 2012

Italian Journal of Animal Science

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In this study the effect of six activating buffers on the movement parameters of sperm were determined and short-term storage of semen in TLP buffer was attempted (0.292 g NaCl; 0.012g KCl; 0.011g CaCl2; 0.004g MgCl2; 0.105g NaHCO3; 0.002g NaH2PO4; 50 mL; pH 8.6). Sperm was collected from five orfe individual, and spermiation was stimulated by means of an intraperitoneal Ovopel injection. The basic parameters of spermatozoa motility were measured after the semen was diluted with six different activating solutions, previously used successfully in other fish species. The motility analysis was conducted on a Crismas apparatus. Additionally, short-term storage of semen in TLP buffer was attempted. Subsequently, motility parameters were verified after 0 (Control), 24 and 120 h of storage at 4°C. It has been found that Lahnsteiner's buffer (100 mM NaCl, 10 mM Tris, 0.5% albumin, 199 mOsmkg -1 ) was found to be the most effective in sperm activation. In this paper, the spermatozoa motility of colourful ide is indicated for the first time. Finally, there was a successful attempt at short-term sperm storage for five days. For artificial insemination, it is very important to select the most effective solution to stimulate sperm motility. Data regarding sperm manipulation of orfe are scarce, so the aim of the study was to determine the basic sperm quality parameters of the colour ide form, i.e. Leuciscus idus aberr orfus.

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Semen of Perca fluviatilis L.: Sperm volume and density, seminal plasma indices and effects of dilution ratio, ions and osmolality on sperm motility

Cited by 82 publications

References 34 publications

Treated Wastewater Effluent Reduces Sperm Motility Along an Osmolality Gradient

Treated Wastewater Effluent Reduces Sperm Motility Along an Osmolality Gradient

Dose inseminante para fertilização artificial de ovócitos de dourado

Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

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