Selfreproduction Induced Variability in Agronomic Traits for a Wild Agaricus bisporus

Moquet, Frédéric; Guedes-Lafargue, M. Ramos; Mamoun, M.; Olivier, Jean-Marc

doi:10.2307/3761322

Cited by 6 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While it is feasible in A. bisporus var. bisporus (Moquet et al 1998), because of the inbreeding depression, it is unlikely to obtain non-sister nuclei heterokaryotic single spore isolates that would be as performant as the parent in term of yield but phenotypically different.…”

Section: Homoallelism Inbreeding Depression and Consequences For Thmentioning

confidence: 99%

“…bisporus, genetic improvement of the strains is based on selection among heterokaryotic offspring (intramictic generations) for the past hundred years, and this is possible because the parental heterozygosity is highly conserved (monospore selection, Kerrigan 1993;Moquet et al 1998) while phenotypical variations are observed. It is due among other reasons to the random segregation of the centromeres during the meiosis which makes that the homologous chromosomes are randomly redistributed in the two non-sister nuclei of the heterokaryons (Sonnenberg et al 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Brito

Foulongne-Oriol

Moinard

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Strain breeding is much less advanced in the edible and medicinal species Agaricus subrufescens than in Agaricus bisporus, the button mushroom. Both species have a unifactorial system of sexual incompatibility, a mating type locus tightly linked to a centromere, and basidia producing both homokaryotic (n) and heterokaryotic (n + n) spores. In A. bisporus, breeding is mainly based on direct selection among the heterokaryotic offspring and on hybridization between homokaryotic offspring. The parental heterozygosity is highly maintained in the heterokaryotic offspring due to suppression of recombination and preferential pairing in the spores of nuclei, each one per second meiotic divisions; such "non-sister nuclei" heterokaryons are fertile. In A. subrufescens, recent studies revealed that recombination is not suppressed and that nuclei from the same second meiotic division can also be paired in a spore that give rise to a "sister nuclei" heterokaryon in which the nuclei bear the same mating type allele. The objective of the present work was to investigate the potential function of the different categories of spores in A. subrufescens and their possible use in a genetic breeding program. Using eight co-dominant molecular markers, we found that half of the offspring of the A. subrufescens strain WC837 were heterokaryotic, one quarter of them being sister nuclei heterokaryons. These heterokaryons were infertile and behaved like homokaryons, being even able to cross between each other. In contrast, non-sister nuclei heterokaryons could fruit but inconsistently due to inbreeding depression. Potential roles of these two categories of heterokaryons in nature and consequences for strain breeding are discussed.

show abstract

Section: Homoallelism Inbreeding Depression and Consequences For Thmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Brito

Foulongne-Oriol

Moinard

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…SCAR markers resulted from PCR amplification using one or two primers as follows: PR1 ((1)5Ј-CCCCATACCTTTGCTTCGTC-3Ј and (2) (2)CGCAT-GATGCAAGAAGAA). DNA extraction was conducted as described by Moquet et al (1998). Volume of amplification was adjusted with water to 25 µl, except for PR8, PR10, and PR14 for which volume was adjusted with water to 100 µl.…”

Section: Scar Markersmentioning

confidence: 99%

“…RAPD were generated as previously described by Moquet et al (1998). Primers from Operon Inc. were chosen in series OPA and OPZ.…”

Section: Rapd Markersmentioning

confidence: 99%

A Quantitative Trait Locus of Agaricus bisporus Resistance to Pseudomonas tolaasii Is Closely Linked to Natural Cap Color

Moquet

Desmerger

Mamoun

et al. 1999

Fungal Genetics and Biology

Self Cite

View full text Add to dashboard Cite

Growing Agaricus bisporus as a Contribution to Sustainable Agricultural Development

Savoie

Mata

2016

Mushroom Biotechnology

View full text Add to dashboard Cite

Selfreproduction Induced Variability in Agronomic Traits for a Wild Agaricus bisporus

Cited by 6 publications

References 0 publications

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens

A Quantitative Trait Locus of Agaricus bisporus Resistance to Pseudomonas tolaasii Is Closely Linked to Natural Cap Color

Growing Agaricus bisporus as a Contribution to Sustainable Agricultural Development

Contact Info

Product

Resources

About