Selfing and sibship structure in a two-cohort stand of maritime pine 
(<i>Pinus pinaster</i> Ait.) using nuclear SSR markers

González‐Martínez, Santiago C.; Gerber, Sophie; Cervera, Marı́a Teresa; Martínez-Zapater, J. M.; Alı́a, Ricardo; Gil, Luis

doi:10.1051/forest:2003003

Cited by 19 publications

(19 citation statements)

References 42 publications

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“…A similar value (96%) was also estimated in two studies using P. pinaster natural stands and chloroplast and nuclear microsatellites (González-Martínez et al, 2003;Lucas et al, 2008). In the three plots, the mean multilocus estimates of outcrossing rate and paternity analysis gave similar results, considering that the paternity analysis values overlapped the mean multilocus estimates upper values (Tab.…”

Section: Mating Systemsupporting

confidence: 67%

“…A similar result (3.8%) was obtained in an adult stand of P. pinaster by using the same set of SSR (González-Martínez et al, 2003). Nevertheless, in the CSO we studied, outcross events among trees with the same genotype were possible for several ramets of the same clone (in Fig.…”

Section: Mating Systemsupporting

confidence: 58%

“…Indeed, a certain level of biparental inbreeding is common, as shown by differences between the multilocus estimate of outcrossing and the average of the single-locus estimate using progeny arrays, in different Pinus species, though low genetic relatedness was found in P. pinaster natural stands (González-Martínez et al, 2003;Lucas et al, 2008). Forest trees are known to suffer from high inbreeding depression (Williams and Savolainen, 1996), and both biparental inbreeding and selfing may have harsh consequences in this species.…”

Section: Mating Systemmentioning

confidence: 99%

“…Plomion et al (2001) observed that the minimum pollen contamination rate was 36%, in a P. pinaster polycross seed orchard (PSO), using chloroplast microsatellites, but the studied orchard block was bordered by other PSO blocks and by mature P. pinaster natural stands. Minimum pollen flow within a P. pinaster natural stand was around 30% based only on exclusion analysis, but the true level of pollen gene flow was probably much higher (González-Martínez et al, 2003). Reports about pollen contamination in seed orchards demonstrated that gene flow can be extensive 706p7 Ann.…”

Section: Gene Flowmentioning

confidence: 99%

“…Parducci et al, 2001). In P. pinaster, according to González-Martínez et al (2003), the carriers of most lethal or sublethal alleles are probably eliminated during seed formation and germination, as well as during the first growing season. The hypothesis of genotyping problems due to the presence of null alleles for those loci was discarded because very low F-values after genotyping 76 adult trees and 132 progenies (seedlings and saplings) were found in a study using the same three loci and the same species .…”

Section: Genetic Variationmentioning

confidence: 99%

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Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

et al. 2008

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Abstract• Relatedness among parents, variation in clonal fertility and background pollination deviate the realized genetic gain and the gene diversity of open pollinated seed orchard from expectation, in particular in wind pollinated species such as Pinus pinaster Aiton.• This work investigates the genetic variation, the mating system and the pollen contamination in a P. pinaster clonal seed orchard (CSO), by screening the 60 clones from the CSO and the seeds collected from 21 mother-trees with three nuclear microsatellites.• The expected diversity was similar, but the observed heterozygosity decreased 20% in the progenies compared with the parental trees. The outcrossing rate was 90.1%, the biparental inbreeding 21.7% computed through a multilocus approach, and the observed selfing 3.9%. The observed gene flow from outside the CSO was 52.4%.• From the results we concluded that the observed gene flow and the biparental inbreeding were high, and care should be taken in the implementation and management of future CSO, in particular clones should be checked for relatedness and the ramet number could be directly proportional to their breeding value. • L'apparentement entre parents, la variation de fertilité entre clones et la pollution pollinique font dévier le gain génétique réalisé et la diversité génétique des valeurs attendues en verger à graines de clones à pollinisation libre, en particulier pour une espèce à pollinisation anémophile comme Pinus pinaster Aiton. Mots-clés• Ce travail étudie la variation génétique, le système de reproduction et la contamination du pollen d'un verger à graines de clones (VGC) de P. pinaster, en génotypant les 60 clones du VGC et les graines récoltées sur 21 arbres-mères pour trois marqueurs microsatellites nucléaires.• La diversité attendue s'est révélée similaire mais l'hétérozygotie observée a diminué de 20 % chez les descendants par rapport aux parents. Le taux d'allofécondation était de 90,1 %, la consanguinité biparentale calculée grâce à une approche multilocus était de 21,7 % et le taux d'autofécondation observé était de 52,4 %.• D'après ces résultats, nous avons conclu que les flux de gènes observés et la consanguinité biparentale étaient élevés et que des précautions devaient être prises dans la mise en place et la gestion de futurs VGC, l'apparentement entre clones devrait en particulier être vérifié et le nombre de copies d'un individu pourrait être directement proportionnel à sa valeur génétique.

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Section: Mating Systemsupporting

confidence: 67%

Section: Mating Systemsupporting

confidence: 58%

Section: Mating Systemmentioning

confidence: 99%

Section: Gene Flowmentioning

confidence: 99%

Section: Genetic Variationmentioning

confidence: 99%

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Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

et al. 2008

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Kosteletzkya virginica displays mixed mating in response to the pollinator environment despite strong inbreeding depression

Ruan

Mopper³

2008

Plant Ecol

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Mixed mating systems often evolve despite strong inbreeding depression, but there have been few experimental tests of this observation. Kosteletzkya virginica, a perennial dicot halophytic species of the Family Malvaceae, is native to brackish portions of coastal tidal marsh in the United States of America and was introduced into China in 1993. Pollination of K. virginica occurs through insect vectors and by delayed self-pollination via stylar movement. In the same two naturalized populations of K. virginica during 2005-2007, we measured (i) inbreeding depression across a range of life-cycle stages, (ii) pollination failure rate, and (iii) selfing rate estimated by AFLP markers. Results indicate that inbreeding depression values for the two naturalized populations during 2005-2007 ranged from 0.620 ± 0.036 to 0.656 ± 0.032, and mean inbreeding depression was 0.640 ± 0.006. Pollinator failure rates at the two naturalized K. virginica populations during 2005-2007 ranged from 0.091 ± 0.039 to 0.174 ± 0.061, and selfing rate ranged from 0.247 ± 0.018 to 0.468 ± 0.031. Population selfing rates are significantly and positively correlated with field estimates of pollinator failure (P = 0.008 \ 0.01, R 2 = 0.857). Our data provide an empirical demonstration of a mixed mating system response to the pollinator environment in the presence of strong inbreeding depression.

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Use of molecular markers for estimating breeding parameters: a case study in a Pinus pinaster Ait. progeny trial

Gaspar

de-Lucas

Alı́a

et al. 2009

Tree Genetics & Genomes

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The management of a genetic improvement program is based on the knowledge of the genetic parameters and their relationships to determine the genetic gains. Knowledge of the coefficient of coancestry (θ) is a requirement for efficient progeny testing scheme and for estimating additive variance components for any quantitative trait. When using open-pollinated families, most authors assume that the seedlings are related as halfsibs, but this is not always true. Our aim was to estimate a mean value of the coancestry coefficient of the families present in a maritime pine Pinus pinaster Ait. (maritime or cluster pine) progeny trial originating from seed collected in a clonal seed orchard and to study how deviations from the standard assumption of θ=0.125 affect heritability estimations. Five highly polymorphic microsatellite markers were scored in 125 offspring from a subsample of five families from the progeny trial. The mean value of the coancestry coefficient of the families present in this progeny trial was 0.130. Differences between the unadjusted and adjusted heritability estimates were more pronounced in wood density (0.609 and 0.586, respectively) than in diameter (0.166 and 0.154, respectively). We conclude that in the trial, the associated error in heritability estimates due to the inclusion of full-sibs, when assuming a standard coefficient of relationship among open-pollinated sibs of 0.250, was low and that this result is robust with respect to the number of families sampled, given unbiased estimates of average relationship among offspring within sib families.

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Selfing and sibship structure in a two-cohort stand of maritime pine (Pinus pinaster Ait.) using nuclear SSR markers

Cited by 19 publications

References 42 publications

Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

Kosteletzkya virginica displays mixed mating in response to the pollinator environment despite strong inbreeding depression

Use of molecular markers for estimating breeding parameters: a case study in a Pinus pinaster Ait. progeny trial

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