Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

Wu, Chien‐Wei; Harroun, Scott G.; Lien, Chia-Wen; Chang, Huan‐Tsung; Unnikrishnan, Binesh; Lai, Irving Po-Jung; Chang, Jia‐Yaw; Huang, Chih‐Ching

doi:10.1016/j.snb.2015.12.045

Cited by 25 publications

(6 citation statements)

References 66 publications

(83 reference statements)

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“…An alternative explanation would be that the presence of SWCNTs alters the plating current density, that is known to affect the concentrations of impurities [42]. We note that unlike XPS (Figure 3) no Cu(OH) 2 is observed in the XRD (Figure 4) [43]; however, given the surface nature of XPS, this would suggest that the Cu(OH) 2 is formed by surface hydrolysis rather than as a bulk material. The most interesting observation is that, as suggested by XPS, the identity of the oxide is dependent on the presence of the SWCNTs.…”

Section: Compositionmentioning

confidence: 83%

Electrodeposition of Cu–SWCNT Composites

Raja

Esquenazi

Gowenlock

et al. 2019

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Single walled carbon nanotubes (SWCNTs) are used as a component of a plating solution of CuSO4 for direct current electrodeposition of Cu–SWCNT composites with varying nanotube proportions without the use of either a surfactant, a dispersing agent, or functionalization of the SWCNTs. The Cu–SWCNT composites are characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Raman spectroscopy. The composites are comprised of metallic Cu and SWCNTs with minor oxide impurities, as well as the residual (Fe) catalyst from the unpurified SWCNTs, in addition to displaying nanotube-mediated morphological differences. EDX analysis of carbon (wt%) is close to quantitative with respect to the wt% of SWCNTs added to the electrolysis solution. The presence of SWCNTs decreases the oxidation of the copper, as well as changing the identity of the oxide from CuO, for electrolysis of Cu, to Cu2O. Hard adherent Cu–SWCNT coatings are prepared by the addition of Cu powder to the electrolysis solution. The approach described in this paper will enable controlled synthesis of metal-nanomaterial composites that can potentially be processed further into high ampacity electrical conductors.

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Section: Compositionmentioning

confidence: 83%

Electrodeposition of Cu–SWCNT Composites

Raja

Esquenazi

Gowenlock

et al. 2019

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“…After that discovery, Fe 3 O 4 NPs were further investigated for their application as peroxidase mimics for detection of H 2 O 2 and other biologically important target molecules. , Tremel and his co-workers showed that V 2 O 5 nanowires possess catalytic activity toward peroxidase substrates, and Zhao et al and Jia et al studied CeO 2 on TiO 2 nanotubes and Co 3 O 4 , respectively, as effective peroxidase mimics. , To add to the list, Cu is the cheapest noble metal and is nontoxic, abundant, and a precursor for copper oxides and metal-based nanomaterials, and it has therefore been widely exploited for use in catalysis, sensors, and biomedicine. − However, very little is known about the properties of CuO with regard to its enzymatic activity. After the report of Chen et al that introduced the peroxidase activity of CuO, efforts have been made to improve this activity. , Recently, Huang and his co-workers synthesized aptamer-functionalized CuO to enhance its peroxidase activity for protein and tumor cell detection …”

Section: Introductionmentioning

confidence: 99%

Cu_0.89Zn_0.11O, A New Peroxidase-Mimicking Nanozyme with High Sensitivity for Glucose and Antioxidant Detection

Nagvenkar

Gedanken

2016

ACS Appl. Mater. Interfaces

206

112

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Nanomaterial-based enzyme mimetics (nanozymes) is an emerging field of research that promises to produce alternatives to natural enzymes for a variety of applications. The search for the most cost-effective and efficient inorganic nanomaterials, such as metal oxides, cannot be won by pristine CuO. However, unlike CuO, the Zn-doped CuO (Zn-CuO) nanoparticles reported in this paper reveal superior peroxidase-like enzyme activity. This places Zn-CuO in a good position to participate in a range of activities aimed at developing diverse enzyme applications. The peroxidase-like activity was tested and confirmed against various chromogenic substrates in the presence of H2O2 and obeyed the Michaelis-Menten enzymatic pathway. The mechanism of enhanced enzymatic activity was proved by employing terephthalic acid as a fluorescence probe and by electron spin resonance. The nanozyme, when tested for the detection of glucose, showed a substantial enhancement in the detection selectivity. The limit of detection (LOD) was also decreased reaching a limit as low as 0.27 ppm. Such a low LOD has not been reported so far for the metal oxides without any surface modifications. Moreover, the nanozyme (Zn-CuO) was utilized to detect the three antioxidants tannic acid, tartaric acid, and ascorbic acid and the relative strength of their antioxidant capacity was compared.

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“…In a more recent parallel development, the inorganic mimics of natural enzymes (NanoZymes) have received intensive attention with a promise to catalyze biologically relevant reactions. − This interest in NanoZymes (nanoparticles as artificial enzymes) is due to the low synthesis cost, high ambient stability, amenity to scale up, tunable catalytic properties, − and, in certain cases, selective toxicity against bacteria over mammalian systems. Such properties have recently seen the exploration of NanoZymes for a range of applications, with a strong focus on sensing and diagnostics. − Lately, a new direction of research has emerged where the enzyme-like catalytic activity of nanoparticles has been assessed for its potential in bacterial control. − This concept of NanoZyme-mediated antibacterial activity stems from the ability of nanoparticles to catalyze conversion of hydrogen peroxide (H 2 O 2 ) to hydroxyl radicals ( • OH), a reactive oxygen species (ROS) that may then participate in the antibacterial activity. − However, one of the major limitations of the current approach is that most of the known NanoZymes do not depend on an external “trigger” to allow tunability of their antibacterial activity.…”

mentioning

confidence: 99%

Visible-Light-Triggered Reactive-Oxygen-Species-Mediated Antibacterial Activity of Peroxidase-Mimic CuO Nanorods

Karim¹,

Singh²,

Weerathunge³

et al. 2018

ACS Appl. Nano Mater.

157

103

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The rapid emergence of antibiotic-resistant bacterial strains warrants new strategies for infection control. NanoZymes are emerging as a new class of catalytic nanomaterials that mimic the biological action of natural enzymes. The development of photoactive NanoZymes offers a promising avenue to use light as a “trigger” to modulate the bacterial activity. Visible light activity is particularly desirable because it contributes to 44% of the total solar energy. Here we show that the favorable band structure of a CuO-nanorod-based NanoZyme catalyst (band gap of 1.44 eV) allows visible light to control the antibacterial activity. Photomodulation of the peroxidase-mimic activity of CuO nanorods enhances its affinity to H2O2, thereby remarkably accelerating the production of reactive oxygen species (ROS) by 20 times. This photoinduced NanoZyme-mediated ROS production catalyzes physical damage to the bacterial cells, thereby enhancing the antibacterial performance against Gram-negative-indicator bacteria Escherichia coli.

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Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

Cited by 25 publications

References 66 publications

Electrodeposition of Cu–SWCNT Composites

Electrodeposition of Cu–SWCNT Composites

Cu_0.89Zn_0.11O, A New Peroxidase-Mimicking Nanozyme with High Sensitivity for Glucose and Antioxidant Detection

Visible-Light-Triggered Reactive-Oxygen-Species-Mediated Antibacterial Activity of Peroxidase-Mimic CuO Nanorods

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Self-templated formation of aptamer-functionalized copper oxide nanorods with intrinsic peroxidase catalytic activity for protein and tumor cell detection

Cited by 25 publications

References 66 publications

Electrodeposition of Cu–SWCNT Composites

Electrodeposition of Cu–SWCNT Composites

Cu0.89Zn0.11O, A New Peroxidase-Mimicking Nanozyme with High Sensitivity for Glucose and Antioxidant Detection

Visible-Light-Triggered Reactive-Oxygen-Species-Mediated Antibacterial Activity of Peroxidase-Mimic CuO Nanorods

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Cu_0.89Zn_0.11O, A New Peroxidase-Mimicking Nanozyme with High Sensitivity for Glucose and Antioxidant Detection