Self-referenced RGB colour imaging of intracellular oxygen

Wang, Xudong; Gorris, Hans H.; Stolwijk, Judith A.; Meier, Robert J.; Groegel, Dominik B. M.; Wegener, Joachim; Wolfbeis, Otto S.

doi:10.1039/c0sc00610f

Cited by 99 publications

(98 citation statements)

References 23 publications

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“…Such an approach has been described for dye encapsulation, for example in silicate or polystyrene spheres but the advantage of this approach is that the probes are physically separated between core and shell of the nanoparticle, which can reduce potential probe cross-talk. 67 While lectine binding experiments provide a conceptional proof for selective binding, in vitro cellular binding of the nanoparticles was investigated in CHO cells. Carbohydrate receptors such as lectins are prevalent on cell surfaces, and CHO-K1 was used in the present study because this cell line has been demonstrated previously to express relatively large amounts of Galectin 1, which resides at the plasma membrane and can bind to extracellular glycoconjugate ligands.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Facile Synthesis of Fluorescent Latex Nanoparticles with Selective Binding Properties Using Amphiphilic Glycosylated Polypeptide Surfactants

et al. 2014

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Section: ■ Results and Discussionmentioning

confidence: 99%

Facile Synthesis of Fluorescent Latex Nanoparticles with Selective Binding Properties Using Amphiphilic Glycosylated Polypeptide Surfactants

et al. 2014

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“…Fluorescent nanosensors are composed of a porous biofriendly polymeric matrix, such as polyacrylamide, 54 silica sol-gel, 52 polystyrene derivatives, 55 and poly(methyl methacrylate), 56 and usually present with dimensions that are less than pulled optical fibers, but greater than free fluorophores. The large surface area of the nanosensor permits a small volume of sensing elements to interact with a large number of analytes.…”

Section: Polymeric Fluorescent Nanosensorsmentioning

confidence: 99%

Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review)

Harrison

Chauhan

2017

Biointerphases

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Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in clinical trial outcomes, the path to wide-scale adoption of CGTs remains challenging, such that the emergence of a “blockbuster” therapy has so far proved elusive. Manufacturing solutions for these therapies require the application of scalable and replicable cell manufacturing techniques, which differ markedly from the existing pharmaceutical incumbent. Attempts to adopt this pharmaceutical model for CGT manufacture have largely proved unsuccessful. The most significant challenges facing CGT manufacturing are process analytical testing and quality control. These procedures would greatly benefit from improved sensory technologies that allow direct measurement of critical quality attributes, such as pH, oxygen, lactate and glucose. In turn, this would make manufacturing more robust, replicable and standardized. In this review, the present-day state and prospects of CGT manufacturing are discussed. In particular, the authors highlight the role of fluorescent optical sensors, focusing on their strengths and weaknesses, for CGT manufacture. The review concludes by discussing how the integration of CGT manufacture and fluorescent optical sensors could augment future bioprocessing approaches.

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“…As the signal intensities in a well are affected by the experimental -in particular coating -conditions, the intact peptide was used as a reference signal to obtain ratiometric measurements that are essentially independent of absolute signal intensities. 25,26 The ratio of the signal aer renin incubation to the reference signal thus denes the degree of proteolytic degradation.…”

Section: Renin Assaymentioning

confidence: 99%

Kinetic analysis of renin and its inhibitors by detecting double-labelled peptidic substrates with an immunoassay

Gorris

2013

Analyst

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The proteolytic activity of renin is a key element in the regulation of blood pressure and a main target for inhibitor design. Currently, the activity of renin and its inhibitors is mainly analyzed using radioimmunoassays or FRET-substrates, which both have their limitations. Here, a novel kinetic assay is presented that combines the advantages of a homogeneous proteolytic reaction and a robust heterogeneous detection in a sandwich immunoassay format. The proteolysis in solution is not influenced by surface interactions and yields accurate kinetic values, while the specific detection of the cleavage products on a microtiter plate strongly reduces interference by concomitant substances and allows for a self-referenced signal readout. A new enzyme kinetic scheme for the inhibition of renin has been developed and validated by using the model inhibitor pepstatin. This kinetic analysis is amenable to parallelization for large-scale inhibitor screening. Furthermore, it can be easily adapted to inhibitors of other medically important proteases.

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Self-referenced RGB colour imaging of intracellular oxygen

Cited by 99 publications

References 23 publications

Facile Synthesis of Fluorescent Latex Nanoparticles with Selective Binding Properties Using Amphiphilic Glycosylated Polypeptide Surfactants

Facile Synthesis of Fluorescent Latex Nanoparticles with Selective Binding Properties Using Amphiphilic Glycosylated Polypeptide Surfactants

Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review)

Kinetic analysis of renin and its inhibitors by detecting double-labelled peptidic substrates with an immunoassay

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