Self-neutralizing PLGA/magnesium composites as novel biomaterials for tissue engineering

Xu, Thomas O; Kim, Hyun S.; Stahl, Tyler; Nukavarapu, Syam P.

doi:10.1088/1748-605x/aaaa29

Cited by 35 publications

(21 citation statements)

References 67 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, other factors may have limited the bone regeneration response, including the slower degradation rate of higher molecular weight PLGA that may have occupied space, discouraging bone tissue ingrowth. The combination of PLGA and Mg alloy mesh might be expected to moderate the side effects from acidic degradation products of PLGA because of the alkaline corrosion products of Mg as measured in previous publications (Wen, Zou, Luo, & Zhou, ; Wu, Ibrahim, et al, ; Wu, Li, et al, ; Xu, Kim, Stahl, & Nukavarapu, ). It is not clear how the PLGA and Mg mesh components will specifically interact with each other to accelerate or slow the degradation of each material in vivo.…”

Section: Discussionmentioning

confidence: 86%

Hybrid scaffolds of Mg alloy mesh reinforced polymer/extracellular matrix composite for critical-sized calvarial defect reconstruction

Chen

Sato

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

The challenge of developing scaffolds to reconstruct critical-sized calvarial defects without the addition of high levels of exogenous growth factor remains relevant. Both osteogenic regenerative efficacy and suitable mechanical properties for the temporary scaffold system are of importance. In this study, a Mg alloy mesh reinforced polymer/demineralized bone matrix (DBM) hybrid scaffold was designed where the hybrid scaffold was fabricated by a concurrent electrospinning/electrospraying of poly(lactic-co-glycolic acid) (PLGA) polymer and DBM suspended in hyaluronic acid (HA). The Mg alloy mesh significantly increased the flexural strength and modulus of PLGA/DBM hybrid scaffold. In vitro results demonstrated that the Mg alloy mesh reinforced PLGA/DBM hybrid scaffold (Mg-PLGA@HA&DBM) exhibited a stronger ability to promote the proliferation of bone marrow stem cells (BMSCs) and induce BMSC osteogenic differentiation compared with control scaffolding materials lacking critical components. In vivo osteogenesis studies were performed in a rat critical-sized calvarial defect model and incorporated a variety of histological stains and immunohistochemical staining of osteocalcin. At 12 weeks, the rat model data showed that the degree of bone repair for the Mg-PLGA@HA&DBM scaffold was significantly greater than for those scaffolds lacking one or more of the principal components. Although complete defect filling was not achieved, the improved mechanical properties, promotion of BMSC proliferation and induction of BMSC osteogenic differentiation, and improved promotion of bone repair in the rat critical-sized calvarial defect model make Mg alloy mesh reinforced PLGA/DBM hybrid scaffold an attractive option for the repair of critical-sized bone defects where the addition of exogenous isolated growth factors is not employed.

show abstract

Section: Discussionmentioning

confidence: 86%

Hybrid scaffolds of Mg alloy mesh reinforced polymer/extracellular matrix composite for critical-sized calvarial defect reconstruction

Chen

Sato

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…Histologically, compared to the PLGA/TCP group, the PPS group exhibited more osteoid tissue and mineralised and mature lamellar bone at weeks 4 and 8, which indicates that the use of PPS may assist bone repair. The release of Mg ion from PPS could enhance bone healing [20], [35], [36]. The PPS was degraded more rapidly than the PLGA/TCP scaffold at weeks 8 and 12, as reflected by the percentages of residual SVs revealed by micro-CT, which indicates that the local microenvironment favoured rapid new bone formation with replacement of the scaffold.…”

Section: Discussionmentioning

confidence: 95%

Use of a three-dimensional printed polylactide-coglycolide/tricalcium phosphate composite scaffold incorporating magnesium powder to enhance bone defect repair in rabbits

Long

et al. 2019

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

BackgroundThe repair of large bone defects remains challenging for orthopaedic surgeons. Bone grafting remains the method of choice; such grafts fill spaces and enhance bone repair. Therapeutic agents also aid bone healing. The objective of this study is to develop a composite bioactive scaffold composed of polylactide-coglycolide (PLGA) and tricalcium phosphate (TCP) (the basic carrier) incorporating osteogenic, bioactive magnesium metal powder (Mg).MethodPorous PLGA/TCP scaffolds incorporating Mg were fabricated using a low-temperature rapid-prototyping process. We term the PLGA/TCP/Mg porous scaffold (hereafter, PPS). PLGA/TCP lacking Mg served as the control material when evaluating the efficacy of PPS. A total of 36 New Zealand white rabbits were randomly divided into blank, PLGA/TCP (P/T) and PPS group, with 12 rabbits in each group. We established bone defects 15 mm in length in rabbit radii to evaluate the in vivo osteogenic potential of the bioactive scaffold in terms of the direct controlled release of osteogenic Mg ion during in vivo scaffold degradation. Radiographs of the operated radii were taken immediately after implantation and then at 2, 4, 8 and 12 weeks. Micro-computed tomography of new bone formation and remaining scaffold and histological analysis were performed at 4, 8, 12 weeks after operation.ResultsX-ray imaging performed at weeks 4, 8 and 12 post-surgery revealed more newly formed bone within defects implanted with PPS and PLGA/TCP scaffolds than blank group (p < 0.05). And micro-computed tomography performed at weeks 4 and 8 after surgery revealed more newly formed bone within defects implanted with PPS scaffolds than PLGA/TCP scaffolds (p < 0.05). Histologically, the PPS group had more newly mineralized bone than controls (p < 0.05). The increases in new bone areas (total implant regions) in the PPS and PLGA/TCP groups were 19.42% and 5.67% at week 4 and 48.23% and 28.93% at week 8, respectively. The percentages of remaining scaffold material in total implant regions in the PPS and PLGA/TCP groups were 53.30% and 7.65% at week 8 and 20.52% and 2.70% at week 12, respectively.ConclusionOur new PPS composite scaffold may be an excellent orthopaedic substitute; it exhibits good biocompatibility and may potentially have clinical utility.Translational potential of this articleMagnesium and beta-tricalcium phosphate had osteoinduction. It is significant to print a novel bone composite scaffold with osteoinduction to repair segmental bone defects. This study evaluated efficacy of PPS in the rabbit radius segmental bone defect model. The results showed that the novel scaffold with good biocompatibility may be an excellent graft and potentially have clinical utility.

show abstract

“…High-density pellet cultures were treated with chondrogenic conditioned media consisting of high-glucose DMEM/F12 with Glutamax, supplemented with 10 nM TGF-b 1 , ITS +1 , 50 mg/mL ascorbate-2-phosphate, 100 mg/mL Na-pyruvate, 40 mg/mL proline, 100 nM dexamethasone, and 1% P/S, and cultured for 14 days. 42,52 The resulting pellets were fixed, paraffin embedded, sectioned, and stained with 1% Alcian blue solution to examine qualitatively sulfated glycosaminoglycan production as a marker for chondrogenic differentiation. 52 Uncultured cells (25 mL/sample) were maintained in adipogenic media using 24-well tissue culture plates.…”

Section: Trilineage Differentiationmentioning

confidence: 99%

“…42,52 The resulting pellets were fixed, paraffin embedded, sectioned, and stained with 1% Alcian blue solution to examine qualitatively sulfated glycosaminoglycan production as a marker for chondrogenic differentiation. 52 Uncultured cells (25 mL/sample) were maintained in adipogenic media using 24-well tissue culture plates. Cells were cultured for a 72-h cycle in DMEM/F12 with Glutamax media containing 10% FBS, 1% P/S, 0.2 mM indomethacin, 50 mg/ mL ascorbate-2-phosphate, 1 nM dexamethasone, 0.1 mg/mL insulin, and 1 mM isobutyl-1-methylxanthine (IBMX).…”

Section: Trilineage Differentiationmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Autologously Derived Biomaterials and Stem Cells for Bone Tissue Engineering

Mikael

Golebiowska

Kumbar

et al. 2020

Tissue Engineering Part A

View full text Add to dashboard Cite

Despite progress, clinical translation of tissue engineering (TE) products/technologies is limited. A significant effort is underway to develop biomaterials and cells through a minimally modified process for clinical translation of TE products. Recently, bone marrow aspirate (BMA) was identified as an autologous source of cells for TE applications and is currently being tested in clinical therapies, but the isolation methods need improvement to avoid potential for contamination and increase progenitor cell yield. To address these issues, we reproducibly processed human peripheral blood (PB) and BMA to develop autologously derived biomaterials and cells. We demonstrated PB-derived biomaterial/gel cross-linking and fibrin gel formation with varied gelation times as well as biocompatibility through support of human bone marrow-derived stem cell survival and growth in vitro. Next, we established a plastic culture-free process that concentrates and increases the yield of CD146 + /CD271 + early mesenchymal progenitor cells in BMA (concentrated BMA [cBMA]). cBMA exhibited increased colony formation and multipotency (including chondrogenic differentiation) in vitro compared with standard BMA. PB-derived gels encapsulated with cBMA also demonstrated increased cell proliferation and enhanced mineralization when assessed for bone TE in vitro. This strategy can potentially be developed for use in any tissue regeneration application; however, bone regeneration was used as a test bed for this study.

show abstract

Self-neutralizing PLGA/magnesium composites as novel biomaterials for tissue engineering

Cited by 35 publications

References 67 publications

Hybrid scaffolds of Mg alloy mesh reinforced polymer/extracellular matrix composite for critical-sized calvarial defect reconstruction

Hybrid scaffolds of Mg alloy mesh reinforced polymer/extracellular matrix composite for critical-sized calvarial defect reconstruction

Use of a three-dimensional printed polylactide-coglycolide/tricalcium phosphate composite scaffold incorporating magnesium powder to enhance bone defect repair in rabbits

Evaluation of Autologously Derived Biomaterials and Stem Cells for Bone Tissue Engineering

Contact Info

Product

Resources

About