Self-nanoemulsifying drug delivery system of fisetin: Formulation, optimization, characterization and cytotoxicity assessment

A Solid Ultra Fine Self-Nanoemulsifying Drug Delivery System (S-SNEDDS) of Deferasirox for Improved Solubility: Optimization, Characterization, and In Vitro Cytotoxicity Studies

“…The maximum cell death was produced at 40 μM of pure DFX where 3.99% cell viability was detected, while, at the same concentration, the cell viability percentage (%) of DFX loaded SNEDDS formulation (P5-40) was 71.44%, suggesting a least/non-toxic effect. This effect of the developed DFX loaded SNEDDS formulation is likely due to the formation of nanoemulsion upon dilution where DFX remained in the globule as an oil/water emulsion and resulted in less interaction with the cells [ 31 ].…”

“…Thereafter, these cells were treated with 10, 30, and 40 μM of DFX loaded SNEDDS formulation (P5-40), placebo SNEDDS (P5°), and pure DFX and incubated for 24 h in 5% CO 2 and at 37 °C. Following this, 10 μL of MTT solution was added into each well, mixed gently, and incubated at 37 °C for 4 h. Then, formazan crystals were dissolved by adding 100 μL DMSO into each well [ 31 ]. The absorbance was read at 570 nm using a microplate reader (VersaMax™, Molecular Devices LLC, San Jose, California, USA).…”

“…To quantify cell viability and proliferation of K562 cells after treatment with selected DFX loaded SNEDDS (P5-40), MTT assay was applied by using Cell proliferation Kit I (MTT) (Roche, Penzberg, Germany); 100 µL of K562 cells at a concentration of 4 × 10 4 cells/mL in culture medium were seeded into each well of a flat-bottomed 96-well plate and incubated 24 h in 5% CO 2 and at 37 • C. Thereafter, these cells were treated with 10, 30, and 40 µM of DFX loaded SNEDDS formulation (P5-40), placebo SNEDDS (P5 • ), and pure DFX and incubated for 24 h in 5% CO 2 and at 37 • C. Following this, 10 µL of MTT solution was added into each well, mixed gently, and incubated at 37 • C for 4 h. Then, formazan crystals were dissolved by adding 100 µL DMSO into each well [31]. The absorbance was read at 570 nm using a microplate reader (VersaMax™, Molecular Devices LLC, San Jose, California, USA).…”

<p>Oral Nano Drug Delivery Systems for the Treatment of Type 2 Diabetes Mellitus: An Available Administration Strategy for Antidiabetic Phytocompounds</p>

“… 183 Nowadays, research is moving towards some novel applications of SNEDDS to make up for their shortcomings, such as self-double emulsions (w/o/w), solid SNEDDS, controlled release SNEDDS, supersaturated SNEDDS, targeted SNEDDS. 175 , 184 , 185 For example, solid SNEDDS are more stable and amenable to compress into tablets as compared to their liquid counterparts. 186 Garg et al 86 solidified liquid SNEDDS of polypeptide-k using Aerosil 200 as hydrophobic carrier through the spray drying technique.…”

Penggunaan D-Optimal Mixture Design untuk Optimasi dan Formulasi Self-Nano Emulsifying Drug Delivery System (SNEEDS) Asam Mefenamat

“…Dalam beberapa tahun terakhir, penerapan desain eksperimental statistik telah berubah menjadi alat yang berguna dalam merancang dan mengoptimasi formulasi dan proses farmasi karena memiliki kemampuan untuk menyelidiki pengaruh variabel formulasi dan efek interaksinya pada karakteristik yang diinginkan [11]. Pendekatan desain eksperimental telah dilaporkan dan efektif untuk optimasi pemilihan formula SNEDDS andrografolida dengan simplex lattice design [12], fisetin dan gemfibrozil dengan Box-Behnken design [13,14].…”