Self-grafting copper oxide nanoparticles show a strong enhancement of their anti-algal and anti-yeast action

Halbus, Ahmed F.; Horozov, Tommy S.; Paunov, Vesselin N.

doi:10.1039/c9na00099b

Cited by 26 publications

(32 citation statements)

References 71 publications

(83 reference statements)

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“…A variety of different NPs such as Au NPs, silica NPs, Mg(OH) 2 NPs and CuONPs have all been shown to demonstrate increased toxicity towards bacteria. [20][21][22][23][24]45 AgNPs has been researched in anti-biofilm treatments due to the silver ions intrinsic antimicrobial property. 25,43 Nanocarriers for antimicrobial agents have also been shown to increase their efficacy against a wide range of microorganisms, including resistant species [26][27][28][29] and deliver drugs specifically to a bacterial target.…”

Section: Introductionmentioning

confidence: 99%

Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria

Weldrick

Hardman

Paunov

2021

Mater. Chem. Front.

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Section: Introductionmentioning

confidence: 99%

Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria

Weldrick

Hardman

Paunov

2021

Mater. Chem. Front.

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“…The increased proliferation of infectious illnesses that are caused by microorganisms found in food packaging, medical devices, water treatment systems, and domestic appliances has elicited increased interest [1,2,3]. The increased resistance of microorganisms against current biocides has caused great concern, particularly for individuals of compromised immune systems [4,5]. This has prompted expanded efforts to investigate new types of nanomaterials as antibacterial agents [6,7,8], which do not rely on the existing pathways of antimicrobial resistance.…”

Section: Introductionmentioning

confidence: 99%

Controlling the Antimicrobial Action of Surface Modified Magnesium Hydroxide Nanoparticles

2019

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Magnesium hydroxide nanoparticles (Mg(OH)2NPs) have recently attracted significant attention due to their wide applications as environmentally friendly antimicrobial nanomaterials, with potentially low toxicity and low fabrication cost. Here, we describe the synthesis and characterisation of a range of surface modified Mg(OH)2NPs, including particle size distribution, crystallite size, zeta potential, isoelectric point, X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), energy dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). We explored the antimicrobial activity of the modified Mg(OH)2NPs on the microalgae (C. reinhardtii), yeast (S. cerevisiae) and Escherichia coli (E. coli). The viability of these cells was evaluated for various concentrations and exposure times with Mg(OH)2NPs. It was discovered that the antimicrobial activity of the uncoated Mg(OH)2NPs on the viability of C. reinhardtii occurred at considerably lower particle concentrations than for S. cerevisiae and E. coli. Our results indicate that the antimicrobial activity of polyelectrolyte-coated Mg(OH)2NPs alternates with their surface charge. The anionic nanoparticles (Mg(OH)2NPs/PSS) have much lower antibacterial activity than the cationic ones (Mg(OH)2NPs/PSS/PAH and uncoated Mg(OH)2NPs). These findings could be explained by the lower adhesion of the Mg(OH)2NPs/PSS to the cell wall, because of electrostatic repulsion and the enhanced particle-cell adhesion due to electrostatic attraction in the case of cationic Mg(OH)2NPs. The results can be potentially applied to control the cytotoxicity and the antimicrobial activity of other inorganic nanoparticles.

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“…[31,32] Additionally, the complex surface of bacterial cell membranes/walls can lead to non-specific interactions, which may potentially interfere with the detection methods. [33] For the purpose of detection of the presence of a whole bacterium (as opposed to metabolites that indicate their presence), mechanical [34] (impedimetric biosensors) and optical [35] methods have recently been developed. Most mechanical biosensors rely on the measurement of the change in various signal frequencies (depending on detector type) [36] when in the presence of the analyte that is being detected, with the two main categories of mechanical biosensors being based upon using quartz crystal microbalances (QCM) [37] and cantilever technologies.…”

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“…The preparation of the bacterial bioimprint is schematically represented in Figure 1B. [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] The cell templating method is reminiscent of the replication of the surface of particle monolayers in the Gel Trapping Technique (GTT) [46][47][48][49][50][51][52] and also the fabrication of micro-lens arrays from particle monolayers. [53] When the target bacteria present in a sample are incubated with the bioimprint, they bind strongly to its cavities due to the increased contact area between the imprint cavities and the matching cell.…”

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“…This was achieved by using GLYMO/4-HPBA conjugation to the silica nanoparticles surface, as recently developed by Halbus et al [35,44] which allows them to form covalent bonds with cis-diol groups, which are abundantly present on glycoproteins expressed on the bacterial cell walls. Such surface functionality is present on both Gram-negative and Gram-positive bacteria.…”

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Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

2020

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We have developed and tested a novel ELISA-like approach for bacterial detection based upon selective adhesion of targeted bacteria to microwells with prefabricated bacterial bioimprints. Bioimprints were produced from three bacterial species; Escherichia coli (Gram-negative), Rhodococcus rhodochrous (Gram-positive) and Sarcina aurantiaca (Gram-negative), by using molding with curable silicone from dense layers of bacterial cells deposited on a glass substrate. We demonstrated that the surface functionalized whole cell bioimprints were able to selectively recognize and bind their own bacterial cell type. In order to detect target bacteria that are bound to the matching bioimprint, we also developed silica nanoparticles dual-functionalized with (3-glycidyloxypropyl)trimethoxysilane (GLYMO) coupled with 4-hydroxyphenylboronic acid (4-HPBA), SiO 2 NPs/GLYMO/4-HPBA, which were further conjugated with horseradish peroxidase (HRP). Bacterial detection was demonstrated to work in the established ELISA-like protocol using the colorimetric reaction of the conjugated HRP with 3,3′,5,5′tetramethylbenzidine (TMB). The bioimprints were used instead of capture antibodies and HRP-coated dual functionalized silica nanoparticles instead of a secondary antibody with TMB as the enzyme-converted reagent, producing a colored byproduct. This bacterial bioimprint-based detection method does not rely on any antibodies, uses stable and inexpensive reagents, and could potentially find application for rapid diagnostics of bacterial pathogens in clinical samples at the point of care. K E Y W O R D S bacteria, bioimprints, ELISA, functionalized silica nanoparticles, horse radish peroxidase 1 INTRODUCTION Bacterial infections remain as a serious issue in modern health care. [1-3] In recent years, this need for the rapid diagnosis of bacterial infections has resulted in the This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Self-grafting copper oxide nanoparticles show a strong enhancement of their anti-algal and anti-yeast action

Abstract: We report a strong amplification of the anti-algal and anti-yeast action of CuO nanoparticles surface-grafted with 4-hydroxyphenylboronic acid functional groups due to their covalent binding to carbohydrates on the cell membranes.

Cited by 26 publications

References 71 publications

Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria

Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria

Controlling the Antimicrobial Action of Surface Modified Magnesium Hydroxide Nanoparticles

Antibody‐free bioimprint aided sandwich ELISA technique for cell recognition and rapid screening for bacteria

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