Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria

Weldrick, Paul J.; Hardman, Matthew J.; Paunov, Vesselin N.

doi:10.1039/d0qm00874e

Cited by 24 publications

(37 citation statements)

References 47 publications

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“…Treating bacterial infections with species demonstrating antibiotic resistance to the chosen antibiotic is often hindered due to the ability of certain bacteria to grow biofilms where they can effectively hide and resist the antibiotic action. Weldrick et al 147 reported an innovative solution for overcoming both antibiotic resistance and biofilm formation by designing active antibiotic nanocarriers with a protease surface functionality on the shellac nanoparticles loaded with an antibiotic. The cationic protease coating, whilst allowing electrostatic adhesion of the nanoparticle to the cell, simultaneously also degrades the biofilm and helps the active nanocarriers to reach the entrapped bacterial cells (Fig.…”

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

“…16E and F). 147 This concept was demonstrated by encapsulating Penicillin G and Oxacillin into shellac NPs, subsequently coated with the serine Weldrick et al 154 tested the performance of a similar innovative nanotechnological approach on S. aureus, where the results showed a boost in the biomass reduction of the bacterial biofilms compared to treatment with free clindamycin as well as with conventionally used antibacterial agents (cetrimide, benzalkonium chloride, povidone-iodine (PVP-I), and chloroxylenol).…”

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

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Emerging nanotechnologies for targeting antimicrobial resistance

et al. 2022

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Section: Polymeric Nanoparticlesmentioning

confidence: 99%

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

Emerging nanotechnologies for targeting antimicrobial resistance

et al. 2022

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“…In another approach, the nanocarrier-based drug combination therapy has been widely applied for the treatment of biofilm infections by co-delivering antibiotics and biofilmdispersing enzymes, which are capable of degrading EPS components, such as proteins, eDNA, polysaccharides, or quorum-sensing molecules (Table 4) [136][137][138][139]. The dispersing compounds could revert the biofilm bacteria back to their planktonic growth mode, rendering bacteria susceptible to conventional antibiotics.…”

Section: Codelivery Platforms For Combination Antibiotic Therapymentioning

confidence: 99%

Application of Polymeric Nanocarriers for Enhancing the Bioavailability of Antibiotics at the Target Site and Overcoming Antimicrobial Resistance

Karakasyan

Jouenne

et al. 2021

Applied Sciences

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Antimicrobial resistance is one of the greatest threats to global health. Although the efforts in antibiotic drug discovery continue to play a pivotal role, this solution alone probably will not be enough to ensure the required level of infection control in the future. New strategies and innovative modes of action are desperately needed to preserve the effectiveness of antimicrobials. Accordingly, antibiotic delivery based on polymeric nanoparticles is one of the possible methods that has been recently explored to improve their pharmacokinetic profile. Through optimized access of antibiotics to their sites of action, nanocarriers can unlock the full potential of the antibiotic cargoes, extend the antimicrobial spectrum, and reduce the required dose of antibiotic while preserving efficacy. Additionally, the use of an antibiotic-loaded nanocarrier is also considered a steady solution as novel molecules can be continuously developed and incorporated into the delivery platform. This review describes the present state of polymeric nanocarriers in enhancing antibiotic treatment, including improved pharmacokinetic properties and restored antibiotic efficacy against drug-resistant bacteria. Additionally, the current challenges and the future direction of this field are discussed.

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“…Some of these cationic nanoparticle formulations rely on electrostatic attraction with the negatively charged cell walls of microbial cells. Recently, active nanocarriers of both antibiotic and antifungal agents were developed with protease coating that can digest their way through biofilms delivering their payload to the embedded microbial cells [27][28][29]. However, in antimould applications, the strategy of using solely such electrostatic binding to the mould can be challenging due to the presence of other anionic species in the hyphae immediate environment which can render the treatment ineffective.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antimould Action of Surface Modified Copper Oxide Nanoparticles with Phenylboronic Acid Surface Functionality

et al. 2021

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Antimould agents are widely used in different applications, such as specialty paints, building materials, wood preservation and crop protection. However, many antimould agents can be toxic to the environment. This work aims to evaluate the application of copper oxide nanoparticles (CuONPs) surface modified with boronic acid (BA) terminal groups as antimould agents. We developed CuONPs grafted with (3-glycidyloxypropyl) trimethoxysilane (GLYMO), coupled with 4-hydroxyphenylboronic acid (4-HPBA), which provided a strong boost of their action as antimould agents. We studied the antimould action of the 4-HPBA-functionalized CuONPs against two mould species: Aspergillus niger (A. niger) and Penicillium chrysogenum (P. chrysogenum). The cis-diol groups of polysaccharides expressed on the mould cell walls can form reversible covalent bonds with the BA groups attached on the CuONPs surface. This allowed them to bind strongly to the mould surface, resulting in a very substantial boost of their antimould activity, which is not based on electrostatic adhesion, as in the case of bare CuONPs. The impact of these BA-surface functionalized nanoparticles was studied by measuring the growth of the mould colonies versus time. The BA-functionalized CuONPs showed significant antimould action, compared to the untreated mould sample at the same conditions and period of time. These results can be applied for the development of more efficient antimould treatments at a lower concentration of active agent with potentially substantial economic and environmental benefits.

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Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria

Abstract: We developed a novel active nanocarrier of common antibiotics, which can efficiently degrade biofilms of resistant bacteria and bypass their defences.

Cited by 24 publications

References 47 publications

Emerging nanotechnologies for targeting antimicrobial resistance

Emerging nanotechnologies for targeting antimicrobial resistance

Application of Polymeric Nanocarriers for Enhancing the Bioavailability of Antibiotics at the Target Site and Overcoming Antimicrobial Resistance

Enhanced Antimould Action of Surface Modified Copper Oxide Nanoparticles with Phenylboronic Acid Surface Functionality

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