Self‐fertility and resistance to the Colorado potato beetle (Leptinotarsa decemlineata) in a diploid Solanum chacoense recombinant inbred line population

Abstract

A diploid potato (Solanum tuberosum L.) recombinant inbred line population was derived from a cross between Solanum chacoense inbred line M6 and S. chacoense accession USDA8380‐1 (80‐1) to identify loci associated with self‐compatibility and Colorado potato beetle resistance. Individuals from the F4 and F5 generations were genotyped on the Illumina Infinium V3 22K Single Nucleotide Polymorphism (SNP) Array and a genetic map constructed. All F5 individuals contain at least one copy of the dominant S‐locus inhibitor (Sli) haplotype; however, not all F5 individuals set fruit. Pollen tubes reached the ovaries of both self‐fruitful and self‐unfruitful plants, indicating that the presence of the dominant Sli allele is not sufficient for selfed fruit and seed production. Loci on chromosomes 3, 5, 6, and 12 were identified as novel targets for self‐fertility improvement. Evaluation of fruit and seed set upon selfing in the F4 generation over two greenhouse seasons revealed environmental influence on self‐fertility. Loci exhibiting residual heterozygosity were found on all chromosomes except chromosomes 3 and 11 in F5 inbreds, but none of the measured self‐fertility traits were correlated with the level of heterozygosity based on SNP genotyping. Four SNPs on chromosome 2 between 22,151,711 and 22,381,719 bp were associated with foliar leptine glycoalkaloid synthesis and Colorado potato beetle resistance in the recombinant inbred line population. Robust inbred lines carrying Colorado potato beetle resistance were developed without field selection during the inbreeding process and beetle resistance was introgressed into diploid breeding lines.

Identification, Elucidation and Deployment of a Cytoplasmic Male Sterility System for Hybrid Potato

“…Among 2011 BC 2 individuals, we did not find any true recombinants in the 25.12 Mb interval on the short arm of Chromosome 6 ( Table S3 ). The suppression of recombination in this interval on Chromosome 6 is observed in many potato genetic maps, suggesting that this region is pericentromeric and that fine-mapping approaches will not reduce the size of the interval [ 16 , 43 , 54 , 55 , 56 , 57 ]. Thus, identification of the causal gene requires an alternative strategy.…”

“…The Sli gene, which encodes an F-box PP2-B10 protein, is expressed in pollen and interacts with the maternal components of the gametophytic self-incompatibility (GSI) system, the S-RNases, during pollination, leading to a breakdown of the GSI system [ 13 , 14 ]. From a breeding point of view, this system works well and has resulted in the production of inbred lines in multiple programs [ 4 , 5 , 6 , 15 , 16 , 17 , 18 ]. However, as a consequence of the presence of Sli , maternal inbred lines are self-fertile, which necessitates emasculation for hybrid seed production.…”

Converting Hybrid Potato Breeding Science into Practice

“…Many researchers have shown that traits that are selected for in tetraploid breeding programs, were also present in diploid potato germplasm [ 25 , 26 , 27 ]. Tuber shape was for instance mapped to the Ro locus with the round phenotype as the dominant effect [ 28 ], a finding confirmed by Meijer et al [ 26 ] and Jansky and Endelman [ 25 ] in diploid inbred populations.…”