Self-association of oxyhaemoglobin. A nuclear magnetic relaxation study in H2O/D2O solutions

Brnjas-Kraljević, Jasminka; Maričić, Siniša; Bračika, Vlasta

doi:10.1016/0301-4622(77)87008-7

Biophysical Chemistry

1977

DOI: 10.1016/0301-4622(77)87008-7

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Self-association of oxyhaemoglobin. A nuclear magnetic relaxation study in H2O/D2O solutions

Jasminka Brnjas-Kraljević

Siniša Maričić

Vlasta Bračika

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Cited by 5 publications

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Self‐association of hemoglobin: A dielectric dispersion study

Desnica

1979

Biopolymers

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SynopsisThe concentration dependence of the dielectric dispersion between 1 kHz and 0.5 MHz was investigated for dense hemoglobin solutions. The concentration was varied between 4 and 26 mMA. per heme. From the characteristic changes in dielectric parameters at some threshold concentration, we observe that the association of hemoglobin macromolecules occurs. This statement is also confirmed by viscosity measurement data.

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Self‐association of hemoglobin: A dielectric dispersion study

Desnica

1979

Biopolymers

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Quantitative analyse der selbstassoziation von hämoglobinmolekülen anhand der konzentrationsabhängigkeit der spektren

Burkhard

Barnikol

1982

Makromol. Chem.

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The dependence of the spectrum of oxygenated hemoglobin (Hb) on the Hb concentration was measured and it was found that Hb solutions do not obey Lambert-Beer's law. The absorption coefficient depends clearly on the Hb concentration. As all external parameters were kept constant except Hb concentration, the only way to explain our result is to assume that Hb molecules undergo self association and that this self association is reflected in the behaviour of the absorption coefficient. To get parameters which are suited to describe the self association of Hb molecules, two models were used. The first model is based on the idea that Hb associates according to the following equation: Hb + Hb P (Hb),. Hb represents a single Hb molecule (consisting of two a-and two 8-chains) and (Hb), a complex of two Hb molecules. The second, more complicated model, takes into account higher associates of Hb molecules (like (Hb),, (Hb),, . . .). By using a least square fitting, it was possible to match experimental data and theoretical curves. Furthermore it became evident that at Hb concentrations, which are of physiological relevance, about 90% of the Hb molecules are involved in self association. The second model revealed that at those Hb concentrations there is a considerable portion of complexes which are composed of 10 or more Hb molecules.

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Hydration and selfassociation of haemoglobin in solution

Brnjas-Kraljević

Maričić

1978

Biochemical and Biophysical Research Communications

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Self-association of oxyhaemoglobin. A nuclear magnetic relaxation study in H2O/D2O solutions

Cited by 5 publications

References 15 publications

Self‐association of hemoglobin: A dielectric dispersion study

Self‐association of hemoglobin: A dielectric dispersion study

Quantitative analyse der selbstassoziation von hämoglobinmolekülen anhand der konzentrationsabhängigkeit der spektren

Hydration and selfassociation of haemoglobin in solution

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