1979
DOI: 10.1002/bip.1979.360180708
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Self‐association of hemoglobin: A dielectric dispersion study

Abstract: SynopsisThe concentration dependence of the dielectric dispersion between 1 kHz and 0.5 MHz was investigated for dense hemoglobin solutions. The concentration was varied between 4 and 26 mMA. per heme. From the characteristic changes in dielectric parameters at some threshold concentration, we observe that the association of hemoglobin macromolecules occurs. This statement is also confirmed by viscosity measurement data.

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Cited by 10 publications
(4 citation statements)
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“…Firstly, the inclusions of physiological solution are not spheres; in fact, if a suitable shape factor of about 1.75 is used in equation (3), very good agreement can be obtained (figure 3). Secondly, it must be noted that, as pointed out by Desnica (1979), at this high haemoglobin concentration the intermolecular interactions become significant and therefore the dielectric constant as a function of the protein content may deviate from a straight line. Consequently, the observed discrepancy may be an artefact due to the extrapolation of the dielectric constant to high haemoglobin concentrations.…”
Section: And * + 2 and 7 -P And T + 2 Andmentioning
confidence: 94%
“…Firstly, the inclusions of physiological solution are not spheres; in fact, if a suitable shape factor of about 1.75 is used in equation (3), very good agreement can be obtained (figure 3). Secondly, it must be noted that, as pointed out by Desnica (1979), at this high haemoglobin concentration the intermolecular interactions become significant and therefore the dielectric constant as a function of the protein content may deviate from a straight line. Consequently, the observed discrepancy may be an artefact due to the extrapolation of the dielectric constant to high haemoglobin concentrations.…”
Section: And * + 2 and 7 -P And T + 2 Andmentioning
confidence: 94%
“…GI. (12) das Verhalten von tHb hinreichend gut beschreiben kann. Hierzu mufi tklb in Abhangigkeit von FKDI (co) bzw.…”
Section: Auswertung Der Absorptionsmessungenunclassified
“…[1][2][3][4][5][6][7] Several theoretical models have been proposed to relate the experimental findings to the dielectric behavior of globular proteins in solution (ref 8 and references therein). [1][2][3][4][5][6][7] Several theoretical models have been proposed to relate the experimental findings to the dielectric behavior of globular proteins in solution (ref 8 and references therein).…”
Section: Introductionmentioning
confidence: 99%
“…The protein solutions' dielectric relaxation studies carried out during the last fifty years have provided important information about the potential relaxation mechanisms. Several theoretical models have been proposed to relate the experimental findings to the dielectric behavior of globular proteins in solution (ref and references therein). The dielectric decrement ( I = ε 0 − ε ∞ / g ) and the relaxation time of native BSA were found to increase on the addition of a high concentration of urea up to 8 M, although the dielectric properties of the denatured state were not drastically different from those of the native protein (thus indicating that urea-denatured BSA still maintains considerable amounts of the original folding of peptide chains).…”
Section: Introductionmentioning
confidence: 99%