Self-association and gel formation during sedimentation of like-charged colloids

Abstract

Formation of superstructures from a colloidal dispersion involves a continuous increase in particle concentration resulting in more and more complicated interparticle interactions. At high concentration, the presence of super-crowding effect,...

Versatile Capillary Cells for Handling Concentrated Samples in Analytical Ultracentrifugation

“…Among techniques capable of measuring the physical states of macromolecules at high concentrations, analytical ultracentrifugation (AUC) is preeminent as a first-principle method to precisely characterize macromolecules in real-time. Especially, AUC-sedimentation equilibrium, AUC-SE, is a popular AUC technique based on the equilibrium between sedimentation and diffusion of the sample, which is well-known for its ability to study intermolecular interactions. − …”

Amino Acids and Their Biological Derivatives Modulate Protein–Protein Interactions in an Additive Way

“…They have distinct molecular weights and isoelectric points (14 000 Da and pH 11 for lysozyme; 66 000 Da and pH 4.5 for BSA). We use the method of sedimentation-diffusion equilibrium analytical ultracentrifugation (SE-AUC) − as an analytical method to measure the second virial coefficient ( B 22 ), , which parameter reflects the extent of the solution nonideality (i.e., protein self-interaction here). We find a general modulation effect on PPIs of a variety of AAs’ biological derivatives.…”