Self‐Assembly of Nanoparticles on Live Bacterium: An Avenue to Fabricate Electronic Devices

Berry, Vikas; Saraf, Ravi F.

doi:10.1002/anie.200501711

Cited by 110 publications

(93 citation statements)

References 36 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the PEI-coated Ag@MESs did associate with bacilli, the negatively charged particles showed a slightly increased association to bacilli, in contrast to the result observed with the E. coli experiments. While this result is unexpected for many Gram-positive bacteria that display negatively-charged teichoic acid on the peptidoglycan layer, [44] the B. antharacis strain used produces a proteinacious, crystalline S-layer that surrounds the bacillus, and likely governs interactions with nanoparticles. [45] This observation also correlates with the viability assay experiments in which the Ag@MESs were able to slow the growth of B. anthracis at 50 mg mL…”

mentioning

confidence: 81%

Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles

2009

View full text Add to dashboard Cite

MCM-41 type mesoporous silicate particles have attracted a significant amount of research interest due to the ordered porous structure of the materials, their facile synthetic methods, and their broad range of applications. [1][2][3][4] Further developments in the synthesis and modification of nanosized mesoporous silica materials have created new possibilities for biomedical applications. [5][6][7][8] As opposed to nonporous silica nanoparticles, both the surface and the pore interior of mesostructured nanoparticles can be modified with functional groups, such that they become compatible in various solutions and are able to store different types of molecules. [9][10][11][12][13][14][15] These nanomaterials have been well demonstrated for their biocompatibility, [16,17] and in their utilization as fluorescent markers for cells, [18] gene-transfection agents, [19] and delivery vehicles for proteins and anticancer drugs. [20,21] Studies of the interaction of mesoporous silicate nanoparticles with bacteria are rare. The nanoparticles were modified with dye molecules to enable studies of possible interaction using fluorescence microscopy. Rhodamine B isothiocyanate (RITC) was reacted with aminopropyltriethoxysilane and mixed with the silica precursor tetraethylorthosilicate (TEOS) to functionalize the interior pores and surface of the particles with the dye molecules without disrupting the mesostructure. [6,21,22] For our previous studies, two types of bacteria were used: Bacillus anthracis BH450 (B. anthracis), as the Gram-positive model, and Escherichia coli BL21 DE3 (E. coli), as the Gram-negative model. Upon mixing the nanoparticles with bacteria, red fluorescence of the nanoparticles was found to overlap with B. anthracis, but not with E. coli, suggesting that the particle adherence to the bacteria may depend on the bacterial strain and surface characteristic of the nanoparticles (Fig. S1, Supporting Information). Encouraged by these initial results and the report on use of nitric oxidereleasing silica nanoparticles as bactericidal agents, [23] we continued the studies in order to develop mesostructured silica nanocomposites that can store large amounts of antimicrobial materials and slowly release the bactericidal agents over an extended time period.The ability to encapsulate inorganic materials by growing mesostructured silica around them introduces additional functionality to the nanoparticles. Gold, semiconductor, and iron oxide nanocrystals can be incorporated within mesoporous silica nanoparticles to create a yolk/shell architecture without collapsing the mesostructure. [24][25][26][27] In comparison to the traditional coating of inorganic nanocrystals with nonporous silica or polymer, [28][29][30][31][32] the mesoporous silica shell offers the advantage of being able to store molecules or to slowly release the encapsulated inorganic materials. [33,34] Based on the works by Trewyn et al. in synthesizing mesoporous nanoparticles that release bactericidal cationic surfactants, [35] and Jiang et al. in prepar...

show abstract

mentioning

confidence: 81%

Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles

2009

View full text Add to dashboard Cite

show abstract

“…The bioinorganic hybrid nanostructures combine the optical, electronic, and mechanical properties of inorganic nanomaterials with the good biocompatibility and low cost of natural biomaterials, which may find potential applications in optics, [1,2] electronics, [3][4][5] magnetics, [6,7] mechanics, [8] catalysts, [9] and battery materials. [10] While 1D inorganic composite nanostructures have been realized by functionalizing various 1D inorganic nanomaterials such as nanotubes, [11][12][13][14] nanowires (NWs), [15][16][17] or nanofibers [18] with nanoparticles (NPs), quite a few 1D biological materials such as tissues, [19] live fungus [9,20] and bacteria, [3][4][5]21] virus, [10,[22][23][24] and biomolecules [25][26][27] have been used to produce 1D bioinorganic hybrid materials.…”

Section: Introductionmentioning

confidence: 99%

Biotemplated Synthesis of Gold Nanoparticle–Bacteria Cellulose Nanofiber Nanocomposites and Their Application in Biosensing

Zhang

Wang

Zhang

et al. 2010

Adv Funct Materials

338

190

View full text Add to dashboard Cite

Bacteria cellulose (BC) nanofibers are used as robust biotemplates for the facile fabrication of novel gold nanoparticle (NP)–bacteria cellulose nanofiber (Au–BC) nanocomposites via a one‐step method. The BC nanofibers are uniformly coated with Au NPs in aqueous suspension using poly(ethyleneimine) (PEI) as the reducing and linking agent. With the addition of different halides, Au–BC nanocomposites with different Au shell thicknesses are formed, and a possible formation mechanism is proposed by taking into account the special role played by PEI. A novel H2O2 biosensor is constructed using the obtained Au–BC nanocomposites as excellent support for horseradish peroxidase (HRP) immobilization, which allows the detection of H2O2 with a detection limit lower than 1 µM. The Au–BC nanocomposites could be further used for the immobilization of many other enzymes, and thus, may find potential applications in bioelectroanalysis and bioelectrocatalysis.

show abstract

“…9 Very recently, a similar approach utilizing electric field assisted oriented assembly of CdS nanorods has also been demonstrated. 10 Biological elements in the form of DNA, 11 bacteria, 12 and viruses 13 have also been used as templates for assembly of nanoscale elements. These techniques all have limitations for assembly of nanoelements on large centimeter scales or for rapid assembly and utilize expensive and time consuming processes.…”

mentioning

confidence: 99%

High-throughput assembly of nanoelements in nanoporous alumina templates

Gultepe

Nagesha

Menon

et al. 2007

Applied Physics Letters

View full text Add to dashboard Cite

The authors demonstrate a nanofabrication method utilizing nanoporous alumina templates which involves directed three dimensional assembly of nanoparticles inside the pores by means of an electrophoretic technique. In their demonstration, they have assembled polystyrene nanobeads with diameter of 50 nm inside nanopore arrays of height of 250 nm and diameter of 80 nm. Such a technique is particularly useful for large-scale, rapid assembly of nanoelements for potential device applications. © 2007 American Institute of Physics. ͓DOI: 10.1063/1.2730575͔ Nanomaterials in the form of nanoparticles, nanowires, and nanotubes are the main components in the burgeoning field of nanotechnology. Due to their unique physical and chemical properties, these nanoelements have the potential for use in a variety of applications such as memory devices, 1 chemical sensors, 2 biosensors, 3,4 in diagnostics, 5 and imaging. 6 However, an important challenge in nanomanufacturing is the directed growth or assembly of nanoelements over macroscopic length scales. Different strategies have been explored to address the two dimensional ͑2D͒ and three dimensional ͑3D͒ assemblies of nanomaterials. Using capillary forces, spherical colloidal particles ͑Ͼ150 nm͒ have been assembled in microfabricated patterns of various shapes. 7 Sub-50 nm colloids were assembled on a lithographically patterned surface by a mechanism known as capillary force assembly. 8 Another approach involves bias induced assembly. For example, CdS colloids have been assembled within the trenches created on block copolymers by application of bias voltage. 9 Very recently, a similar approach utilizing electric field assisted oriented assembly of CdS nanorods has also been demonstrated. 10 Biological elements in the form of DNA, 11 bacteria, 12 and viruses 13 have also been used as templates for assembly of nanoscale elements. These techniques all have limitations for assembly of nanoelements on large centimeter scales or for rapid assembly and utilize expensive and time consuming processes. Assembly of nanoparticles into macroscopic structures that have all dimensions in the nanometer length scale is still quite a formidable challenge.In this letter we report a different kind of nanoassembly technique utilizing nanoporous alumina templates. Charged nanoparticles in solution are assembled inside the pores by means of an electrophoretic process. This method is the demonstration of directed assembly in truly 2D nanostructures. It also demonstrates a very simple technique for rapid and large-scale assembly of nanoelements.Ordered nanoporous alumina templates were prepared by anodization of aluminum foil in an acid solution. For this, a commercially available Al foil was used as anode, graphite sheet was used as cathode, and 5 wt % oxalic acid as the electrolyte. Anodization was carried out at 4°C for 6 h under a constant of 50 V direct current ͑dc͒ potential. This produced a thick layer ͑ϳtens of microns͒ of porous aluminum oxide consisting of an array of vertically arranged pores ...

show abstract

Self‐Assembly of Nanoparticles on Live Bacterium: An Avenue to Fabricate Electronic Devices

Cited by 110 publications

References 36 publications

Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles

Antimicrobial Activity of Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles

Biotemplated Synthesis of Gold Nanoparticle–Bacteria Cellulose Nanofiber Nanocomposites and Their Application in Biosensing

High-throughput assembly of nanoelements in nanoporous alumina templates

Contact Info

Product

Resources

About