Self-Assembly of Mesoscale Artificial Clathrin Mimics

Kong, Yifan; Hanna, Mina-Elraheb; Zhuo, Denys; Chang, Katherine G.; Bozorg-Grayeli, Tara; Melosh, Nicholas A.

doi:10.1021/acsnano.7b03739

Cited by 8 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2,3 In addition to its biological significance, the structural elegance of clathrin has 3 inspired the development of artificial functional materials that mimic clathrin lattices and their self-assembly. [4][5][6] DNA is increasingly exploited as a building material for predictable and precise assembly of various structures on the nanoscale. DNA nanostructures can be conceived by specifically designing the individual nucleotide sequences, which then form unique shapes aided by the Watson-Crick base-pairing interactions.…”

Section: Main Textmentioning

confidence: 99%

Clathrin-Inspired Coating and Stabilization of Liposomes by DNA Self-Assembly

Baumann¹,

Piantanida²,

García-Nafría³

et al. 2019

Preprint

View full text Add to dashboard Cite

The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis in biological systems and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of clathrin self-assemblies to coat liposomes with biomaterials, new classes of hybrid carriers can be derived. Here we present a method for fabricating DNA-coated liposomes by hydrophobically anchoring and subsequently growing a DNA network on the liposome surface which structurally mimics clathrin assemblies. Dynamic light scattering (DLS), ζ-potential and cryo-electron microscopy (cryo-EM) measurements independently demonstrate successful DNA coating. Nanomechanical measurements conducted with atomic force microscopy (AFM) show that the DNA coating enhances the mechanical stability of the liposomes relative to uncoated ones. Furthermore, we provide the possibility to reverse the coating process by triggering the disassembly of the DNA coating through a toehold-mediated displacement reaction. Our results describe a straightforward, versatile, and reversible approach for coating and stabilizing lipid vesicles by an interlaced DNA network. This method has potential for further development towards the ordered arrangement of tailored functionalities on the surfaces of liposomes and for applications as hybrid nanocarrier.

show abstract

Section: Main Textmentioning

confidence: 99%

Clathrin-Inspired Coating and Stabilization of Liposomes by DNA Self-Assembly

Baumann¹,

Piantanida²,

García-Nafría³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In its monomeric form, clathrin has the shape of a triskelion. Upon an external stimulus, it forms spherical complexes surrounding a membrane vesicle with an alternating pattern of hexagons and pentagons. , In addition to its biological significance, the structural elegance of clathrin has inspired the development of artificial functional materials that mimic clathrin lattices and their self-assembly. − …”

mentioning

confidence: 99%

Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly

et al. 2020

View full text Add to dashboard Cite

The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of the protein building blocks and clathrin self-assemblies to coat liposomes with biomaterials, advanced hybrid carriers can be derived. Here, we present a method for fabricating DNA-coated liposomes by hydrophobically anchoring and subsequently connecting DNA-based triskelion structures on the liposome surface inspired by the assembly of the protein clathrin. Dynamic light scattering, ζpotential, confocal microscopy, and cryo-electron microscopy measurements independently demonstrate successful DNA coating. Nanomechanical measurements conducted with atomic force microscopy show that the DNA coating enhances the mechanical stability of the liposomes relative to uncoated ones. Furthermore, we provide the possibility to reverse the coating process by triggering the disassembly of the DNA coats through a toehold-mediated displacement reaction. Our results describe a straightforward, versatile, and reversible approach for coating and stabilizing lipid vesicles through the assembly of rationally designed DNA structures. This method has potential for further development toward the ordered arrangement of tailored functionalities on the surface of liposomes and for applications as hybrid nanocarriers.

show abstract

“…An interesting proposal by Melosh and co-workers demonstrated the control of microscopic motions of microscopic objects as mesoscale artificial clathrin mimics at the air−water interface (Figure 7). 64 In this example, three-leg bimetallic structures were selfassembled at liquid interfaces. The assembled structures have dynamic natures, and their structural changes and dimensional transformations can be induced by external stimuli such as magnetic fields, sometime resulting in fluidic sampling.…”

Section: Recent Progress Of Advanced Analysis Of Materials Nanoarchit...mentioning

confidence: 99%

Langmuir Nanoarchitectonics from Basic to Frontier

2018

View full text Add to dashboard Cite

Methodology to combine nanotechnology and these organization processes has been proposed as a novel concept of nanoarchitectonics, which can fabricate functional materials with nanolevel units. As an instant nanoarchitectonics approach, confining systems within a two-dimensional plane to drastically reduce translational motion freedom can be regarded as one of the rational approaches. Supramolecular chemistry and nanofabrication and their related functions at the air-water interface with the concept of nanoarchitectonics would lead to the creation of a novel methodology of Langmuir nanoarchitectonics. In this feature article, we briefly summarize research efforts related to Langmuir nanoarchitectonics including the basics for anomalies in molecular interactions such as highly enhanced molecular recognition capabilities. It is also extended to frontiers including the fabrication of supramolecular receptors and two-dimensional patterns with subnanometer-scale structural regulation, manual control of molecular machines and receptors by hand-motion-like macroscopic actions, and the regulation of cell fates at nanoarchitected arrays of nanocarbon assemblies and at direct liquid interfaces.

show abstract

Self-Assembly of Mesoscale Artificial Clathrin Mimics

Cited by 8 publications

References 52 publications

Clathrin-Inspired Coating and Stabilization of Liposomes by DNA Self-Assembly

Clathrin-Inspired Coating and Stabilization of Liposomes by DNA Self-Assembly

Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly

Langmuir Nanoarchitectonics from Basic to Frontier

Contact Info

Product

Resources

About