Self-assembly of dengue virus empty capsid-like particles in solution

Neves-Martins, Thais Cristtina; Mebus-Antunes, Nathane C.; Neto, Carlos H.G.; Barbosa, Glauce M; Almeida, Fábio C. L.; Caruso, Ícaro Putinhon; Poian, Andrea T. Da

doi:10.1016/j.isci.2023.106197

Cited by 1 publication

(2 citation statements)

References 54 publications

(79 reference statements)

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“…A conserved intermolecular salt bridge has also been identified in DENV, formed by D87/D87′, with a biochemically characterized role in dimerization [ 75 ]. A recent study used DENV C protein-mutated residues R85 and R85′ to bind cysteines, forcing a disulfide bond between the two α 4 helices to establish dimerization [ 76 ]. This mutation formed a dimer stable enough to maintain core-like structures in vitro, although these cores contained no nucleic acid.…”

Section: Structural Features Of the Capsid Proteinmentioning

confidence: 99%

“…A study by Kiermayer et al in 2004 [ 90 ] produced core-like particles using TBEV C; however, the size was 2–3 nm larger than the predicted core diameter of ~30 nm. Stabilization of the dimeric structure through the addition of a forced disulfide bond allowed the formation of core-like particles using DENV C. However, these particles contained no RNA [ 76 ]. Despite these efforts, no specific residues have been implicated in core formation, and the mechanism of core assembly remains unknown.…”

Section: Functions Of Capsid Proteinmentioning

confidence: 99%

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The Dynamic Landscape of Capsid Proteins and Viral RNA Interactions in Flavivirus Genome Packaging and Virus Assembly

Jablunovsky,

Jose

2024

Pathogens

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The flavivirus genus of the Flaviviridae family of enveloped single-stranded RNA viruses encompasses more than 70 members, many of which cause significant disease in humans and livestock. Packaging and assembly of the flavivirus RNA genome is essential for the formation of virions, which requires intricate coordination of genomic RNA, viral structural, and nonstructural proteins in association with virus-induced, modified endoplasmic reticulum (ER) membrane structures. The capsid (C) protein, a small but versatile RNA-binding protein, and the positive single-stranded RNA genome are at the heart of the elusive flavivirus assembly process. The nucleocapsid core, consisting of the genomic RNA encapsidated by C proteins, buds through the ER membrane, which contains viral glycoproteins prM and E organized as trimeric spikes into the lumen, forming an immature virus. During the maturation process, which involves the low pH-mediated structural rearrangement of prM and E and furin cleavage of prM in the secretory pathway, the spiky immature virus with a partially ordered nucleocapsid core becomes a smooth, mature virus with no discernible nucleocapsid. This review focuses on the mechanisms of genome packaging and assembly by examining the structural and functional aspects of C protein and viral RNA. We review the current lexicon of critical C protein features and evaluate interactions between C and gRNA in the context of assembly and throughout the life cycle.

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Section: Structural Features Of the Capsid Proteinmentioning

confidence: 99%

Section: Functions Of Capsid Proteinmentioning

confidence: 99%