The Dynamic Landscape of Capsid Proteins and Viral RNA Interactions in Flavivirus Genome Packaging and Virus Assembly

Abstract

The flavivirus genus of the Flaviviridae family of enveloped single-stranded RNA viruses encompasses more than 70 members, many of which cause significant disease in humans and livestock. Packaging and assembly of the flavivirus RNA genome is essential for the formation of virions, which requires intricate coordination of genomic RNA, viral structural, and nonstructural proteins in association with virus-induced, modified endoplasmic reticulum (ER) membrane structures. The capsid (C) protein, a small but versatile RNA-binding protein, and the positive single-stranded RNA genome are at the heart of the elusive flavivirus assembly process. The nucleocapsid core, consisting of the genomic RNA encapsidated by C proteins, buds through the ER membrane, which contains viral glycoproteins prM and E organized as trimeric spikes into the lumen, forming an immature virus. During the maturation process, which involves the low pH-mediated structural rearrangement of prM and E and furin cleavage of prM in the secretory pathway, the spiky immature virus with a partially ordered nucleocapsid core becomes a smooth, mature virus with no discernible nucleocapsid. This review focuses on the mechanisms of genome packaging and assembly by examining the structural and functional aspects of C protein and viral RNA. We review the current lexicon of critical C protein features and evaluate interactions between C and gRNA in the context of assembly and throughout the life cycle.

The flavivirus protein NS4B recruits thecis-Golgi protein ACBD3 to modify ER-Golgi trafficking for virion release

“…The newly synthesized RNA genomes exit the ROs and are either translated to yield more viral proteins or packaged into virions. The genomes that end up packaged interact with the C protein on the ER membrane to form nucleocapsids (NCs) that then bud into the lumen of the ER and acquire the lipid envelope, and the embedded prM and E proteins [6,7,14,15]. This process yields immature virions that need to undergo protease cleavage and pH-mediated conformational changes to produce infectious virions that are then secreted from the cell [16][17][18][19][20].…”