Self-assembly of collagen peptides into hollow microtubules

Reimer, Armando; Feher, Katie M.; Hernandez, Daniel A.; Slowinska, Katarzyna

doi:10.1039/c2jm16122b

Cited by 12 publications

(9 citation statements)

References 31 publications

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“…With the exception of T5, all peptides were modified with FITC at the N‐terminus (via a β ‐alanine–glycine–glycine linker) to visualize cellular uptake. The FITC linker sequence was designed to prevent possible quenching of the fluorescence when three peptides fold into a helical conformation . The C‐terminus of all peptides was blocked by amidation to avoid unintended reactions.…”

Section: Resultsmentioning

confidence: 99%

Peptide internalization enabled by folding: triple helical cell-penetrating peptides

Shinde

Feher

et al. 2014

J. Pept. Sci.

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Cell-Penetrating Peptides (CPPs) are known as efficient transporters of molecular cargo across cellular membranes. Their properties make them ideal candidates for in vivo applications. However, challenges in development of effective CPPs still exist: CPPs are often fast degraded by proteases and large concentration of CPPs required for cargo transporting can cause cytotoxicity. It was previously shown that restricting peptide flexibility can improve peptide stability against enzymatic degradation and limiting length of CPP peptide can lower cytotoxic effects. Here we present peptides (30-mers) that efficiently penetrate cellular membranes by combining very short CPP sequences and collagen-like folding domains. The CPP domains are hexa-arginine (R6) or arginine/glycine (RRGRRG). Folding is achieved through multiple proline-hydroxyproline-glycine (POG)n repeats that form a collagen-like triple helical conformation. The folded peptides with CPP domains are efficiently internalized, show stability against enzymatic degradation in human serum, and have minimal toxicity. Peptides lacking correct folding (random coil) or CPP domains are unable to cross cellular membranes. These features make triple helical cell penetrating peptides promising candidates for efficient transporters of molecular cargo across cellular membranes.

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Section: Resultsmentioning

confidence: 99%

Peptide internalization enabled by folding: triple helical cell-penetrating peptides

Shinde

Feher

et al. 2014

J. Pept. Sci.

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“…[ 166 ] To overcome these limitations and provide cells with nanostructured scaffolds, nanotechnology‐based strategies have been used to fabricate tissues and vascular‐like structures: [ 164 , 230 ] phase separation and self‐assembly of peptidic domains of biological polymers, as collagen or elastin, have been used as strategies to engineer nanofibers, nanotubes and nanowires for vascular TE applications. [ 231 , 232 ] However, electrospinning is the main nanofabrication technique for vascularized constructs: [ 230 , 233 ] tubular scaffolds have been electrospun by using rotating mandrels or combination with electrospraying to create highly cellularized constructs, [ 234 ] and multilayer core–shell constructs resembling the blood vessels structure have been manufactured by coaxial electrospinning. [ 235 , 236 , 237 ] Electrospun scaffolds for vascular TE have been manufactured with a variety of natural and synthetic polymers and their combination in blends leads to devices with physiologically relevant mechanical behavior while promoting cell adhesion and proliferation.…”

Section: Vascularization Approaches For Physiologically Relevant 3d Modelsmentioning

confidence: 99%

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

et al. 2021

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Vascularization of 3D models represents a major challenge of tissue engineering and a key prerequisite for their clinical and industrial application. The use of prevascularized models built from dedicated materials could solve some of the actual limitations, such as suboptimal integration of the bioconstructs within the host tissue, and would provide more in vivo-like perfusable tissue and organ-specific platforms. In the last decade, the fabrication of vascularized physiologically relevant 3D constructs has been attempted by numerous tissue engineering strategies, which are classified here in microfluidic technology, 3D coculture models, namely, spheroids and organoids, and biofabrication. In this review, the recent advancements in prevascularization techniques and the increasing use of natural and synthetic materials to build physiological organ-specific models are discussed. Current drawbacks of each technology, future perspectives, and translation of vascularized tissue constructs toward clinics, pharmaceutical field, and industry are also presented. By combining complementary strategies, these models are envisioned to be successfully used for regenerative medicine and drug development in a near future.

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“…It is essential to understand, whether the cell-damage is caused either by the complex 1, irradiation or combination of both. To address this issue, third set of stained cells were monitored using a filter of Rhodamine-B fluorescence (λ ex -543 nm), [81,82] (Figure 6a1,b3,c3). During this irradiation, the cells were remained luminescent for 3.55 min (Figure 6a3).…”

Section: Effect On Living Cellsmentioning

confidence: 99%

Sensing of Phosphate and ATP by Lanthanide Complexes in Aqueous Medium and Its Application on Living Cells

et al. 2020

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The design of coordination complexes as synthetic receptors for selective sensing of inorganic anions and nucleoside phosphates in aqueous medium under physiological conditions are important for biomedical research and drug delivery. In this work, two phenanthroline-based luminescent lanthanide complexes; EuLCl 3 (1) and TbLCl 3 (2), were explored for sensing anions such as HPO 4 2À , SO 4 2À , CH 3 COO À , I À , Br À , Cl À , F À , NO 3 À and HSO 4 À with HPO 4 2À exhibiting the strongest emission. Nucleoside phosphates such as ATP, ADP and AMP could be also detected. Both complexes 1 and 2 are highly selective sensing ATP at physiological pH. The detection limit and their corresponding Stern-Volmer quenching constant were calculated for ATP as in the range of 0.2 μM-1 mM and 2.0 × 10 6 M À 1. Complex 1 was applied as a staining agent to study alga cells (Microspora unialgal filamentous cell). The result shows that the ligand L causes a severe cytotoxic effect and induces the cell damage.

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Self-assembly of collagen peptides into hollow microtubules

Cited by 12 publications

References 31 publications

Peptide internalization enabled by folding: triple helical cell-penetrating peptides

Peptide internalization enabled by folding: triple helical cell-penetrating peptides

In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

Sensing of Phosphate and ATP by Lanthanide Complexes in Aqueous Medium and Its Application on Living Cells

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