Self-assembling antimicrobial peptides on nanotubular titanium surfaces coated with calcium phosphate for local therapy

Abstract

Bacterial infection is a serious medical problem leading to implant failure. The current antibiotic based therapies rise concerns due to bacterial resistance. The family of antimicrobial peptides (AMP) is one of the promising candidates as local therapy agents due to their broad-spectrum activity. Despite AMPs receive increasing attention to treat infection, their effective delivery to the implantation site has been limited. Here, we developed an engineered dual functional peptide which delivers AMP as a biomolecular therapeutic agent onto calcium phosphate deposited nanotubular titanium surfaces. Dual functionality of the peptide was achieved by combining a hydroxyapatite binding peptide-1 (HABP1) with an AMP using a flexible linker. HABP functionality of the peptide provided a self-coating property onto the nano-topographies that are designed to improve osteointegration capability, while AMP offered an antimicrobial protection onto the implant surface. We successfully deposited calcium phosphate minerals on nanotubular titanium oxide surface using pulse electrochemical deposition (PECD) and characterized the minerals by XRD, FT-IR, FE-SEM. Antimicrobial activity of the engineered peptide was tested *

Functionalization of hydrophobic surfaces with antimicrobial peptides immobilized on a bio-interfactant layer

“…Layer-by-layer deposition is a common physical immobilization technique applied to hydrophilic materials such as metal oxides, mainly due to electrostatic interactions 16,17 or the adding of a specic surface binding recognition sequence to the peptide. [18][19][20] This method may be applied on hydrophobic polymer surfaces aer applying a process to increase the concentration of functional groups such as hydroxyl and carbonyl groups, and next covalent attachment using click chemistry. 21 Another viable method is solid phase peptide synthesis on polymer resins in which the protected amino acids are incorporated by assembling the peptide sequence from its C-to its N-terminus; however, these methods are time consuming and costly.…”

Recombinant Oncorhyncin II Effect on the Treatment of Methicillin-Resistant Staphylococcus aureus Skin Infection

“…Nowadays, scientists consider the antimicrobial peptides (AMPs) as a new generation of antibiotics for the future. These peptides have high killing power against pathogenic agents such as bacteria, fungi, parasites, and viruses (7)(8)(9). Oncorhyncin II is an AMP secreted from rainbow trout.…”

The influence of nanoporous anodic aluminum oxide on the initial adhesion of Streptococcus mitis and mutans

“…A reduction of the adherence of oral streptococci to anatase‐rich anodic oxidized titanium surfaces has been demonstrated (Dorkhan et al, ). PEEK and titanium surfaces covered with nano‐silver showed antibacterial properties as well as the subsequent application of self‐assembling peptides or PLGA(Ag‐Fe 3 O 4 ) nanoparticles (Liu et al, ; Pokrowiecki et al, ; Yang et al, ; Yazici et al, ).…”