Self-assembled pH-responsive hyaluronic acid–g-poly(l-histidine) copolymer micelles for targeted intracellular delivery of doxorubicin

Qiu, Lipeng; Li, Zhen; Qiao, Mingxi; Long, Miaomiao; Wang, Mingyue; Zhang, Xiaojun; Tian, Chenmin; Chen, Dawei

doi:10.1016/j.actbio.2013.12.025

Cited by 165 publications

(105 citation statements)

References 54 publications

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“…cell culture and animals MCF-7/Adr (DOX resistant), 25 MCF-7 (DOX sensitive and relatively high CD44 receptor expression), 30,31 and HepG2 (relatively low CD44 receptor expression) 32 cells were obtained from Nanjing Kaiji Biotech. Ltd Co. (Jiangsu, People's Republic of China).…”

Section: Wang Et Almentioning

confidence: 99%

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Wang¹,

Guo

et al. 2016

IJN

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Section: Wang Et Almentioning

confidence: 99%

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Wang¹,

Guo

et al. 2016

IJN

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“…In fact, both pH-responsive HA-gpoly(L-histidine) copolymer micelles and folate-conjugated HA-octadecyl (FA-HA-C18) copolymer, developed by Qiu et al and Liu et al respectively, internalized into cells mainly via clathrin-mediated endocytosis. 53,77 In vivo antitumor activity…”

Section: Exploring Uptake Pathways Of Nanoparticlesmentioning

confidence: 99%

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Abbad¹,

Wang²,

Waddad³

et al. 2015

IJN

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Herein, we describe the preparation of a targeted cellular delivery system for morin hydrate (MH), based on a low-molecular-weight hyaluronic acid-poly(butyl cyanoacrylate) (HA-PBCA) block copolymer. In order to enhance the therapeutic effect of MH, D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) was mixed with HA-PBCA during the preparation process. The MH-loaded HA-PBCA “plain” nanoparticle (MH-PNs) and HA-PBCA/TPGS “mixed” nanoparticles (MH-MNs) were concomitantly characterized in terms of loading efficiency, particle size, zeta potential, critical aggregation concentration, and morphology. The obtained MH-PNs and MH-MNs exhibited a spherical morphology with a negative zeta potential and a particle size less than 200 nm, favorable for drug targeting. Remarkably, the addition of TPGS resulted in about 1.6-fold increase in drug-loading. The in vitro cell viability experiment revealed that MH-MNs enhanced the cytotoxicity of MH in A549 cells compared with MH solution and MH-PNs. Furthermore, blank MNs containing TPGS exhibited selective cytotoxic effects against cancer cells without diminishing the viability of normal cells. In addition, the cellular uptake study indicated that MNs resulted in 2.28-fold higher cellular uptake than that of PNs, in A549 cells. The CD44 receptor competitive inhibition and the internalization pathway studies suggested that the internalization mechanism of the nanoparticles was mediated mainly by the CD44 receptors through a clathrin-dependent endocytic pathway. More importantly, MH-MNs exhibited a higher in vivo antitumor potency and induced more tumor cell apoptosis than did MH-PNs, following intravenous administration to S180 tumor-bearing mice. Overall, the results imply that the developed nanoparticles are promising vehicles for the targeted delivery of lipophilic anticancer drugs.

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“…19 Among these responsive bonds, pH-sensitive bond was mostly researched because it is independent of cellular chemical substances and does not require the exact location of tumors for triggered release. 20 It is well known that the physiological pH in cancer cells is lower compared to that in blood and normal tissues, and it is about 6.0 in early endosomes, and reduces to 5.0 during the progression to late endosomes and lysosomes. 21 pH-sensitive PEG-lipid can be degraded even in weak acid, in favor of endosomal escape, followed by cytoplasmic release of the liposome-incorporated drug.…”

Section: Introductionmentioning

confidence: 99%

Anti-EphA10 antibody-conjugated pH-sensitive liposomes for specific intracellular delivery of siRNA

Zang¹,

Ding²,

Zhao³

et al. 2016

IJN

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Therapeutic delivery of small interfering RNA (siRNA) is a major challenge that limits its potential clinical application. Here, a pH-sensitive cholesterol–Schiff base–polyethylene glycol (Chol–SIB–PEG)-modified cationic liposome–siRNA complex, conjugated with the recombinant humanized anti-EphA10 antibody (Eph), was developed as an efficient nonviral siRNA delivery system. Chol–SIB–PEG was successfully synthesized and confirmed with FTIR and 1 H-NMR. An Eph–PEG–SIB–Chol-modified liposome–siRNA complex (EPSLR) was prepared and characterized by size, zeta potential, gel retardation, and encapsulation efficiency. Electrophoresis results showed that EPSLR was resistant to heparin replacement and protected siRNA from fetal bovine serum digestion. EPSLR exhibited only minor cytotoxicity in MCF-7/ADR cells. The results of flow cytometry and confocal laser scanning microscopy suggested that EPSLR enhanced siRNA transfection in MCF-7/ADR cells. Intracellular distribution experiment revealed that EPSLR could escape from the endo-lysosomal organelle and release siRNA into cytoplasm at 4 hours posttransfection. Western blot experiment demonstrated that EPSLR was able to significantly reduce the levels of MDR1 protein in MCF-7/ADR cells. The in vivo study of DIR-labeled complexes in mice bearing MCF-7/ADR tumor indicated that EPSLR could reach the tumor site rather than other organs more effectively. All these results demonstrate that EPSLR has much potential for effective siRNA delivery and may facilitate its therapeutic application.

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Self-assembled pH-responsive hyaluronic acid–g-poly(l-histidine) copolymer micelles for targeted intracellular delivery of doxorubicin

Cited by 165 publications

References 54 publications

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Anti-EphA10 antibody-conjugated pH-sensitive liposomes for specific intracellular delivery of siRNA

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