Self-assembled PEG monolayer based SPR immunosensor for label-free detection of insulin

Gobi, K. Vengatajalabathy; Iwasaka, Hiroyuki; Miura, Norio

doi:10.1016/j.bios.2006.06.012

Cited by 138 publications

(92 citation statements)

References 36 publications

(43 reference statements)

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“…Though this test was performed at the increment of 90 RU, 5 µL of regeneration solution was found to be effective until 600 RU. The incomplete regeneration for higher response was observed, even after several injections of regeneration solution (glycine, pH 2.0) (Gobi et al, 2007). Similar observation was also …”

Section: Selection Of Better Regeneration Conditionsupporting

confidence: 81%

“…SPR is used in various fields such as food analysis (Taylor et al, 2006), pharmaceutical (Hwang et al, 2005;Chavane et al, 2008), environmental monitoring (Matsumoto et al, 2005;Shankaran et al, 2006;Habauzit et al, 2008), medical diagnostics (Haes et al, 2005;Yang et al, 2005;Gobi et al, 2007;Masson et al, 2007) and others (Baird et al, 2002;Beseničar et al, 2006;Katsamba et al, 2006). Previously, SPR was used to know the binding partners and its relative interaction strength for IFNs (Takacs et al, 1999;Asokan et al, 2006;Schmeisser et al, 2007), immune response against IFN-β (Gibbs and Oger, 2008) and in one particular instance to quantify IFN-γ (Stigter et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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A simple method for quantification of interferon-2b through surface plasmon resonance technique

Ramanan¹,

Ling²,

Tey³

et al. 2010

Afr. J. Biotechnol.

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A rapid and efficient immunoassay method for quantification of interferon-α2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding ratio was observed for both the ligand densities. There was no steric hindrance and loss of antibody activity even at higher ligand density (> 22,000 RU). The sensitivity of the assay was increased up to 45% with the increment in ligand density from 15,400 to 22,360 RU. The binding between interferon-α2b and anti-interferon monoclonal antibody was predominantly controlled by mass transfer rate and the relationship was found linear, ranged from 5 to 400 ng/mL. Total cycle time per analysis was less than 8 min and required only 5 µL of sample injection.

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Section: Selection Of Better Regeneration Conditionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

A simple method for quantification of interferon-2b through surface plasmon resonance technique

Ramanan¹,

Ling²,

Tey³

et al. 2010

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…This type of sensor has been employed in various bioassays that range from the detection of analytes related to food quality and increased safety [10][11][12][13][14] to environmental monitoring [15][16][17][18][19][20][21]. This type of sensor has also been used for medical diagnosis regarding the detection of drugs [22][23][24], hormones [25][26][27], and markers for cancer [28][29][30][31][32][33][34][35]. Despite the wide application and high sensitivity of the conventional SPR configuration, these devices have difficulty of setup alignment, which is a great experimental inconvenience [36].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Plasmonic Biosensor for Breast Cancer Antigen Detection

et al. 2015

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Biosensors based on surface plasmon resonance (SPR), operating with the Kretschmann conventional arrangement, have been employed for biomolecular detection of tumor markers. However, the traditional SPR configuration presents some experimental inconveniences that are overcome by using plasmonic substrates based on nanohole arrays manufactured in metallic films. This SPR configuration exhibits the extraordinary optical transmission (EOT) phenomenon, which is explored in the monitoring of binding events that occur on the metal surface. In this work, we proposed a plasmon biosensor based on nanohole arrays built on gold film operating in collinear transmission mode by using spectral investigation for signal transduction. The SPR substrate was coupled to a microfluidic system and showed good sensitivity and linearity. A concentration of 30 ng mL −1 of human epidermal receptor protein-2 (HER2) antigen (associated with breast cancer) was detected using the integrated device; this showed its great potential to be used in tumor diagnosis.

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“…Surface plasmon resonance (SPR) is an extremely powerful tool for monitoring of biomolecular interactions and has been extensively used for the detection of antigen-antibody [35], RNA-protein [36], DNA-protein [37] and DNA-DNA [38] interactions. Several reports on the use of SPR technique for DNA biosensing and its ability for single point mutation detection can be found in the literature [39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Low–medium resolution HLA-DQ2/DQ8 typing for coeliac disease predisposition analysis by colorimetric assay

et al. 2012

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In the reported work, the development of a multiplex colorimetric assay for the low to medium HLA typing of the DQ2 and DQ8 genes is presented. The optimisation of probe design and assay conditions, performed by both surface plasmon resonance and enzyme-linked oligonucleotide assay, are reported.Finally, the performances of the developed typing platform were validated by the analysis of real patient 2 samples. The HLA typing results gave excellent correlation when compared with those obtained using hospital based typing technologies.

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Self-assembled PEG monolayer based SPR immunosensor for label-free detection of insulin

Cited by 138 publications

References 36 publications

A simple method for quantification of interferon-2b through surface plasmon resonance technique

A simple method for quantification of interferon-2b through surface plasmon resonance technique

Microfluidic Plasmonic Biosensor for Breast Cancer Antigen Detection

Low–medium resolution HLA-DQ2/DQ8 typing for coeliac disease predisposition analysis by colorimetric assay

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