Self-assembled micro-organogels for 3D printing silicone structures

O’Bryan, Christopher S.; Bhattacharjee, Tapomoy; Hart, Samuel M.; Kabb, Christopher P.; Schulze, Kyle D.; Chilakala, Indrasena; Sumerlin, Brent S.; Sawyer, W. Gregory; Angelini, Thomas E.

doi:10.1126/sciadv.1602800

Cited by 214 publications

(214 citation statements)

References 43 publications

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“…Angelini et al. prepared assembled micro‐organogels as the supporting matrix, and fine‐tuned the ratios of the two block copolymers to adjust their rheological property to enable gel‐in‐gel DIW. Heating the supporting gel above 60 °C induced a gel‐to‐liquid phase transition, which released the 3D printed object from the supporting matrix.…”

Section: Facilitating 3d Printability To Polymersmentioning

confidence: 99%

Advanced Polymer Designs for Direct‐Ink‐Write 3D Printing

Lin

Tang

et al. 2019

Chemistry A European J

190

161

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The rapid development of additive manufacturing techniques, also known as three‐dimensional (3D) printing, is driving innovations in polymer chemistry, materials science, and engineering. Among current 3D printing techniques, direct ink writing (DIW) employs viscoelastic materials as inks, which are capable of constructing sophisticated 3D architectures at ambient conditions. In this perspective, polymer designs that meet the rheological requirements for direct ink writing are outlined and successful examples are summarized, which include the development of polymer micelles, co‐assembled hydrogels, supramolecularly cross‐linked systems, polymer liquids with microcrystalline domains, and hydrogels with dynamic covalent cross‐links. Furthermore, advanced polymer designs that reinforce the mechanical properties of these 3D printing materials, as well as the integration of functional moieties to these materials are discussed to inspire new polymer designs for direct ink writing and broadly 3D printing.

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Section: Facilitating 3d Printability To Polymersmentioning

confidence: 99%

Advanced Polymer Designs for Direct‐Ink‐Write 3D Printing

Lin

Tang

et al. 2019

Chemistry A European J

190

161

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“…Using a printing set-up costing <$1K, rapid construct formation on the centimeter scale was achieved in a few minutes, and even faster and more complex tissue printing with higher resolution may be accomplished with higher quality printers. As the microgel size decreased, it was possible to build tissue constructs with sophisticated structures due to the medium shearthinning properties upon needle motion, self-healing properties in absence of external strain, and limited diffusion of the printed cells into its pores 26 . In addition, high viability of the printed cells was realized even after additional photocrosslinking of the microgels for long-term structural support.…”

Section: Discussionmentioning

confidence: 99%

Living cell-only bioink and photocurable supporting medium for printing and generation of engineered tissues with complex geometries

Jeon

Lee

Jeong

et al. 2019

Preprint

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Scaffold-free engineering of three-dimensional (3D) tissue has focused on building sophisticated structures to achieve functional constructs. Although the development of advanced manufacturing techniques such as 3D printing has brought remarkable capabilities to the field of tissue engineering, technology to create and culture individual cell only-based high-resolution tissues, without an intervening biomaterial scaffold to maintain construct shape and architecture, has been unachievable to date. In this report, we introduce a cell printing platform which addresses the aforementioned challenge and permits 3D printing and long-term culture of a living cell-only bioink lacking a biomaterial carrier for functional tissue formation. A biodegradable and photocrosslinkable microgel supporting bath serves initially as a fluid, allowing free movement of the printing nozzle for high-resolution cell extrusion, while also presenting solid-like properties to sustain the structure of the printed constructs. The printed human stem cells, which are the only component of the bioink, couple together via transmembrane adhesion proteins and differentiate down tissue-specific lineages while being cultured in a further photocrosslinked supporting bath to form bone and cartilage tissue with precisely controlled structure. Collectively, this system, which is applicable to general 3D printing strategies, is paradigm shifting for printing of scaffold-free individual cells, cellular condensations and organoids, and may have far reaching impact in the fields of regenerative medicine, drug screening, and developmental biology.

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“…As the support medium is shear‐thinning and self‐healing it flows as the extrusion nozzle passes through the medium but maintains sufficient mechanical properties in order to support the extruded filament. This has been demonstrated for the AM of silicon materials, hydrogels, and bioprinting with cell‐laden hydrogels . In total, granular inks present a simple and promising platform to engineer shear‐thinning and self‐healing platforms for AM.…”

Section: Toolbox For Am Of Precision Biomaterialsmentioning

confidence: 97%

“…While granular materials have proven particularly suitable as inks in deposition‐based AM, they can also serve as a support medium during robotic dispensing . In this approach, granular materials have been placed in a receiving reservoir and ink has been deposited using standard robotic dispensing.…”

Section: Toolbox For Am Of Precision Biomaterialsmentioning

confidence: 99%

Additive Manufacturing of Precision Biomaterials

2019

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Biomaterials play a critical role in modern medicine as surgical guides, implants for tissue repair, and as drug delivery systems. The emerging paradigm of precision medicine exploits individual patient information to tailor clinical therapy. While the main focus of precision medicine to date is the design of improved pharmaceutical treatments based on “‐omics” data, the concept extends to all forms of customized medical care. This includes the design of precision biomaterials that are tailored to meet specific patient needs. Additive manufacturing (AM) enables free‐form manufacturing and mass customization, and is a critical enabling technology for the clinical implementation of precision biomaterials. Materials scientists and engineers can contribute to the realization of precision biomaterials by developing new AM technologies, synthesizing advanced (bio)materials for AM, and improving medical‐image‐based digital design. As the field matures, AM is poised to provide patient‐specific tissue and organ substitutes, reproducible microtissues for drug screening and disease modeling, personalized drug delivery systems, as well as customized medical devices.

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Self-assembled micro-organogels for 3D printing silicone structures

Abstract: High-precision 3D printing of liquid silicone is achieved using a new oil-based microgel as a support medium.

Cited by 214 publications

References 43 publications

Advanced Polymer Designs for Direct‐Ink‐Write 3D Printing

Advanced Polymer Designs for Direct‐Ink‐Write 3D Printing

Living cell-only bioink and photocurable supporting medium for printing and generation of engineered tissues with complex geometries

Additive Manufacturing of Precision Biomaterials

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