Self-Assembled Graphene–Dextran Nanohybrid for Killing Drug-Resistant Cancer Cells

Abstract

A nanohybrid based on nanoscale graphene oxide (NGO) and dextran has been designed and employed for effectively killing drug-resistant MCF-7/ADR cells. This graphene-based nanohybrid was readily prepared through π-π interaction of NGO and hematin-terminated dextran (HDex), being denoted as NGO-HDex. It revealed an improved stability in physiological conditions as compared to native NGO. Besides, NGO-HDex could efficiently load doxorubicin (DOX), an anticancer drug, with drug loading capacity of 3.4 mg/mg NGO and liberate the drug with a pH-dependent profile. Cell viability assay indicated that the NGO-HDex displayed lower cytotoxicity against MCF-7/ADR cells as compared to native NGO. DOX-loaded NGO-HDex, however, revealed more efficient killing effect in the cells than free DOX because the nanohybrid caused a higher amount of DOX accumulated in the cells. The results of this study highlight that the NGO-HDex has high potential for killing drug-resistant cancer cells.

Supramolecular design of coordination bonding architecture on zein nanoparticles for pH-responsive anticancer drug delivery

“…After 4 h of incubation, the fluorescence was disseminated in the whole cytoplasm. Due to the self-quenching effect of DOX in NPs, DOX fluorescence is observed only when DOX is released [45,46]. The DOX/zein NPs witnessed slightly decrease in fluorescence intensity when DOX was loaded (Fig.5b), consistent to drug release results.…”

Tailoring stimuli-responsive delivery system driven by metal–ligand coordination bonding

“…55,56 As shown in the CLSM image, the DOX could quickly release from the zein/CMCS NPs, consistent with drug release results. The dramatic decrease in fluorescence intensity was seen when DOX was loaded in Cu 2+ -TA-coated zein/CMCS NPs after 2 h of incubation ( Figure 7B).…”

Inorganic Nanocarriers Overcoming Multidrug Resistance for Cancer Theranostics

“…The adriamycin‐loaded GOs could escape the efflux action of P‐gp to achieve high accumulation of drugs inside cancer cells, resulting in much higher cytotoxicity against MDR MCF‐7/ADR cells compared to free adriamycin. Similarly, hematin‐terminated dextran (HDex) can be attached to the surface of GOs through π–π interaction to form graphene‐based nanohybrids, which can carry sufficient Dox to effectively kill MDR MCF‐7/ADR cells 121. As mentioned above, co‐delivery of MDR‐reversing agents and anticancer drugs is an effective approach to overcome MDR.…”