Self-assembled elastin-like polypeptide fusion protein coacervates as competitive inhibitors of advanced glycation end-products enhance diabetic wound healing

Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing

“…7D, E, and S5A ). On day 7, the wound areas of the hydrogel Ⅲ–IV group were significantly smaller than that of the gauze control group and the HeraDerm group, and the hydrogel Ⅳ-treated wounds were also smaller at day 7 than those in some previous studies [ [51] , [52] , [53] , [54] ]. On day 10, the wound contraction of the hydrogel Ⅱ group was about 4.84 ± 2.19% higher than that of the HeraDerm group, while the wound healing rate of the hydrogel IV group surpassed approximately 20.1 ± 2.48% of that of the HeraDerm group, indicating that the release system of hydrogel Ⅳ could release insulin and celecoxib effective in wounds, and hydrogel Ⅱ containing CS and gelatin can also promote wound healing.…”

“…The anti-inflammatory effect of hydrogel III might be due to the effective release of insulin, and the anti-inflammatory effect of hydrogel IV was mainly attributed to the long-term release of celecoxib in the wound on-demand. In addition, the Maillard reaction caused by continuous hyperglycemia in diabetic patients promoted the accumulation of advanced glycation end products (AGEs), which is also an important factor in chronic diabetic wound non-healing [ 51 ]. AGEs in skin collagen are directly related to long-term glucose control [ 58 ], and topical application of insulin to wounds could reduce the accumulation of AGEs [ 59 ].…”

Histology Scoring System for Murine Cutaneous Wounds

“…In human skin, wounds close through granulation tissue formation whereas wound contraction mediated by the panniculus carnosus muscle occurs in mice before the formation of granulation tissue [19]. The percentage wound closure as evident on a macroscopic level is a widely used measure to indicate either accelerated or delayed healing responses in murine models [21][22][23][24]. Furthermore, there are several limitations associated with the use of images to quantify macroscopic wound closure, especially since nonperpendicular images (the distance and angle where the camera and the reference ruler is positioned) can significantly underestimate surface area [15].…”

Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds

“…In addition, they have been shown to accelerate wound healing [ 6 ]. Damaged skin has a harsh microenvironment with increased reactive oxygen species (ROS) and inflammation, leading to decreased vitality and increased apoptosis, which impede wound healing [ 7 , 8 ]. Therefore, it is essential to improve seed cell vitality for wound healing; determining the mechanisms underlying seed cell vitality may lead to novel approaches to wound healing.…”