Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry

Chemical profile, agaritine and selenium content of Agaricus bisporus

“…On the other hand, the two uncooked preserved samples contained selenium <0001 ppb. These results were similar to the results obtained by Shi et al (2002) and Gergely et al (2005), which showed 0.12-0.3 ppb selenium. The chemical profile of ethanolic extract of the fresh whole mushroom showed the presence of 174 metabolites out of which 13 had significant content from 1.2 -83%; 13 as 1% and the remaining 148 metabolites were less than 1% (w/w).…”

“…They act as a good source of K, Mg, Ca, Cu, Fe, Zn, and P and have low content of Na. Several other compounds have also been detected from A. bisporus as reported by numerous workers (Shi et al 2002;Andersson and Gry 2004;Gergely et al 2005;Ji, et al 2006;Ey et al 2007;WU 2007;Borchers et al 2008;De Barros 2008;Sadiq et al 2008;USCDC 2009;UN 2010).…”

“…Shi et al, (2002) and Gergely et al (2005). (Wang et al 2007;WU et al 2007;USCDC 2009;UN 2010), which include anticancer agents such as fatty acids poly saccharides, and ergosterol (Takaku et al 2001;Ren et al 2008;Koyyalamudi et al 2009);N,N,N-tris (hydrazinecarbonyl) phosphoric triamide, selenium and vaccenic acid (Shi et al 2002; Sosnovsky and Rao 2004; Gergely et al 2005;De Barros 2008;Mattila et al 2010); antihypercholesterol agents such as fatty acids, glycoproteins, sterols and vaccenic acid (Ey et al 2007;Borchers et al 2008;De Barros 2008); antimicrobial agents such as agaritine and alcohols (Janak, et al 2006;Nagadka, et al 2006;Roupas, et al 2010); anti-cardiovascular disease agents such as fatty acids, sterols and pyran derivative (SCB, 2007(SCB, -2010; human health supporting agent's such as fatty acids, sterols and sugar alcohols (Andersson and Gry 2004;Ji, et al 2006); immune enhancer agents such as fatty acids, glycoproteins, polysaccharides and sterols (UN, 2010);…”

“…A. bisporus has many medicinal metabolites responsible for the therapeutic activity for which the treatment and prevention of many human diseases act as anti-cancer agents such as polysaccharides, fatty acids and ergosterol, N,N,Ntris"hydrazine carbonyl" phosphoric triamide, selenium and vaccenic acid; anti-hyper cholesterol agents (fatty acids, glycoproteins, sterols and vaccenic acid); anti-microbial agents (agaritine and alcohols); anti-cardiovascular disease metabolites (fatty acids, sterols and pyran derivative); hepato protective agent (triterpenoids); human health supporting agent's (fatty acids, sterols and sugar alcohols); immune enhancer metabolites (fatty acids, glycoproteins, polysaccharides and sterols). These medicinal metabolites such as β-glucan and G-glucan; polysaccharide K or PSK "protein bound polysaccharide"; phenols; polyketides, triterpenoids and sterols (De Barros, 2008); PSK (UN 2010); triterpenoids, lectins glycoprotein's (WU et al 2007;USCDC 2009;UN 2010); ergothioneine (Ey et al 2007;Borchers et al 2008); selenium (Shi et al 2002;Gergely et al 2005); pyran derivative (SCB, 2007(SCB, -2010; essential fatty acids (Andersson and Gry 2004;Ji, et al 2006) and eicosanoids (WFI 2011 e ). Essential fatty acids in A. bisporus act against aromatase enzyme which used in estrogen production.…”

Selenium Uptake by Edible Oyster Mushrooms (Pleurotus sp.) from Selenium-Hyperaccumulated Wheat Straw

“…The extent of accumulation was higher than the selenium concentration reported in the case of Pleurotus eryngii (4.6 and 9.3 mg Se/g dw) cultivated on substrates supplemented with 5.0 and 10.0 mg Se/g dw of sodium selenite respectively (32), Similar results have recently been reported by da Silva et al (27), where selenium accumulation in P. ostreatus was found to be higher (57.6 mg Se/g dw) than our observations (44.3 mg Se/g dw) with the same species when cultivated on Se-enriched coffee husks supplemented with 3.2 mg/kg of sodium selenite. Total selenium content in enriched L. edodes and Ganoderma lucidium was found to be 46 mg Se/g and 72 mg Se/g respectively (33,34), which lies within the range observed in the present study. The observations clearly demonstrated that the tolerance and uptake of selenium by various species of mushrooms are dependent upon the variety of mushroom species as well as the form of selenium supplementation.…”