Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding

Ezhuthupurakkal, Preedia Babu; Polaki, Lokeswara Rao; Arumugam, Suyavaran; Subastri, Ariraman; Sujatha, Venugopal; Thirunavukkarasu, Chinnasamy

doi:10.1016/j.msec.2017.02.003

Cited by 60 publications

(24 citation statements)

References 54 publications

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“…Similarly, XRD pattern also revealed the amorphous nature of nSe ( figure 3(e)). The pattern depicted a broad 2θ diffraction peak at 23.30°in accordance with the previouly documented literature [45,48,49]. Raman spectrum of nSe in the range of 150 to 600 cm −1 exhibited a prominent resonance peak at 253 cm −1 owing to Se-Se stretching vibrations, confirming the amorphous nature of nSe ( figure 3(f)) [46,[50][51][52].…”

Section: Resultssupporting

confidence: 87%

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC 50 values of 0.21 μg ml −1 and 3.34 μg ml −1 , respectively. Moreover, nSe exhibited significant inhibition in bacterial growth at low minimum inhibitory concentration (MIC) values against Escherichia coli (16 μg ml −1 ), Staphylococcus aureus (32 μg ml −1 ) and Pseudomonas aeruginosa (48 μg ml −1 ) compared to MIC values for standard drug ampicillin. Importantly, nSe did not induce any cytotoxic effects on normal human keratinocytes (HaCaT) at the tested concentrations; representing their biocompatible nature. The data obtained demonstrated the versatility of phytogreen nSe as a potent antioxidant and antibacterial agent to effectively prevent as well as treat multidrug-resistant bacterial infections.

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Section: Resultssupporting

confidence: 87%

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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“…Se‐NPLs biosynthesized using lemon leaf extract were reported as well . In addition to, biosynthesis of Se‐NPLs by Allium sativum was reported . Use the parsley (crispum) leaves aqueous extract to prepare Se‐NPLs as an eco‐friendly method was recently reported…”

Section: Introductionmentioning

confidence: 92%

Eco‐Friendly Approach to Synthesize Selenium Nanoparticles: Photocatalytic Degradation of Sunset Yellow Azo Dye and Anticancer Activity

2019

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This report has two principal goals. First, to synthesis Se nanoparticles (Se-NPLs) via a green approach. Secondly, to explore the photocatalytic activity of Se-NPLs towards the decolorization of sunset yellow (SSY) azo-dye and to test its activity against some types of human cancers. Green synthesis of Se-NPLs from the leaf extracts of Drumstick was developed. Bio-synthesized Se-NPLs were characterized using FTIR, UV-vis, photoluminescence (PL), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy dispersive analysis Xray (EDAX) and transmission electron microscopy (TEM) analyses. The UV-vis absorption maximum between 200 and 400 nm was due to the formation of SPR of Se-NPLs. FTIR revealed the Se-NPLs were synthesized, capped and stabilized with biomolecules present in the plant extracts. Se-NPLs exhibited an excitation peak at 399 nm and produced an emission peak at 599 nm. EDAX profile provided a signal of atomic Se (1.45 Kev).XRD confirmed the crystalline nature of Se-NPLs. SEM and TEM observations show that spherical Se particles appeared with diameters ranging from 23 to 35 nm. A possible mechanism of the reduction of (SeO 3 2 À ) to (Se 0 ) was discussed. The electrical conductivity was measured with temperature and the activation energy was calculated. The photocatalytic study conducted that Se-NPLs have the efficiency to degrade sunset yellow dye and the mechanism of degradation has been proposed. Se-NPLs have been shown to be effective against three types of human cancers (Caco-2 cells, HepG2 cells, and Mcf-7 cells). Se-NPLs are potent anticancer and inhibit the growth of the three cancer cells as indicated by the IC 50 values. To conclude, the green-synthesized Se-NPLs may be a candidate for further evaluation as a chemotherapeutic agent for some human cancer treatment.

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“…This Biomacromolecule acts as a binding template and provides a platform for metallic NPs to stick through its surface [ 13 ]. The interaction of nanoparticle can induce DNA damage and induce cell cycle arrest which in turn adversely affects other functions of DNA such as replication, transcription and finally leading to cell death [ [14] , [15] , [16] ]. Therefore, DNA-ligand interaction is a crucial issue for elucidating the mode of interaction, and provenance of some diseases to design new pharmaceutical agents that are effective in controlling gene expression [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…The main goal of this study is to investigate and elucidate the biological activity of Nano-selenium, especially their interactions with DNA and the most important blood proteins under physiological conditions (pH = 7.40). Although there are some studies on the interaction of biogenic selenium nanoparticles with ct-DNA [ 15 ] but the mechanism of the interaction of Nano-selenium with serum proteins (HSA, HHb, and cyt c) is not clear yet. Such studies need to be perused to get insights into the molecular mechanism of Nano-selenium–proteins and Nano-selenium–DNA interactions.…”

Section: Introductionmentioning

confidence: 99%

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

Shahabadi

Zendehcheshm

Khademi

2021

Biotechnology Reports

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Selenium nanoparticles synthesized in aqueous extract of Allium sativum perturbs the structural integrity of Calf thymus DNA through intercalation and groove binding

Cited by 60 publications

References 54 publications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

Eco‐Friendly Approach to Synthesize Selenium Nanoparticles: Photocatalytic Degradation of Sunset Yellow Azo Dye and Anticancer Activity

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

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