Selenium nanoparticle-enriched biomass of Yarrowia lipolytica enhances growth and survival of Artemia salina

Hamza, Faseela; Vaidya, Amogh; Apte, Mugdha; Kumar, Anuj; Zinjarde, Smita

doi:10.1016/j.enzmictec.2017.07.002

Cited by 27 publications

(7 citation statements)

References 52 publications

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“…The presence of SeNPs in the yeast cell wall was confirmed by TEM ( Figure 8). The differences in the sizes of NPs may result from the difference in Se oxidation [27], although the results obtained are in accordance with the findings of Hamza et al [66]. The size of SeNPs obtained from the biomass of Yarrowia lipolytica NCIM 3589 ranged between 30 and 60 nm.…”

Section: Detection and Characterization Of Senpssupporting

confidence: 90%

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek

Bierła

Jiménez-Lamana

et al. 2020

IJMS

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Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.

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Section: Detection and Characterization Of Senpssupporting

confidence: 90%

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek

Bierła

Jiménez-Lamana

et al. 2020

IJMS

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“…For this reason, Tukey's comparison test was performed between the values of the predicted and experimental be related to the lower bioavailability of the organic selenium in S. cerevisiae [16]. The obtained result was also in agreement with findings of Hamza et al [15]. They also found that some species of Saccharomyces, had selenium tolerance and selenium in lower amounts, could not be significantly inhibit its growth.…”

Section: Optimization Of the Bioethanol Fermentation Conditionssupporting

confidence: 86%

“…Seleniumenriched yeasts, which are known as selenised yeasts, are the base of many consumed supplements and most popular. For examples, enriched yeasts with selenium, such as Saccharomyces cerevisiae, S. bayanus and S. boulardii have been utilized to produce bread, probiotic products and alcoholic beverages [12,14,15]. Several studies indicated that, glutamate in the yeast, can decrease the energy generation and fermentation rate, by altering the mitochondrial structure and dynamics, which those negatively impacts can be prevented by organic selenium such as seleno-cysteine which that is resulted during bio-transformation of inorganic selenium using yeasts such as S. cerevisiae [12,14].…”

Section: Introductionmentioning

confidence: 99%

“…It reveals that organic selenium has low bioavailability [16]. Furthermore, some species of Saccharomyces, such as Yarrowia lipolytica, has selenium tolerance and selenium in lower amounts, cannot significantly inhibit its growth [15]. To the best of our knowledge, there is not any comprehensive study to evaluate effects of selenium salt and culture media concentrations on production of bioethanol through submerged fermentation process using S. cerevisiae and sugar beet molasses.…”

Section: Introductionmentioning

confidence: 99%

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Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production

Faramarzi

Anzabi

Jafarizadeh‐Malmiri

2019

Green Processing and Synthesis

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A bench scale submerged fermentation process was used to bioethanol produce using sugar beet molasses and Saccharomyces cerevisiae, as substrate and microbial strain, respectively. Effects of selenium amount on growth of S. cerevisiae and bioethanol production were evaluated. The obtained results indicated that growth of S. cerevisiae (manifested as turbidity intensity) in the samples containing 0, 5, 10, 15, 20 and 25 μg sodium selenite, during aerobic process, was 0.1707, 0.1678, 0.1679, 0.1664, 0.1627 and 0.160% a.u./h (after 14 h incubation), respectively. Statistical analysis based on compression test indicated that there were insignificant (p > 0.05) differences between growth rate of the yeast in the fermented samples containing S. cerevisiae and 5 to 25 μg selenium salt. Response surface methodology was utilized to evaluate effects of two fermentation parameters namely, amount of selenium (5-25 μg) and substrate brix (10-25°Bx) on the concentration (g/L) of produced bioethanol. Obtained results revealed that maximum bioethanol concentration (55 g/L) was achieved using 15 μg selenium and molasses with 25°Bx. Furthermore, results have also indicated that, without using selenium and using molasses with 25°Bx, bioethanol with concentration of 29 g/L was produced.

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“…This can be considered one of the stages of detoxification of Se by the microorganisms. A study by Hamza et al [ 61 ] showed that Y. lipolytica NCIM 3589 strain could tolerate up to 8.0 mM concentration of selenite sodium. The authors also obtained a red-colored yeast cell mass, which suggested the synthesis of Se in the form of nanoparticles (SeNP).…”

Section: Resultsmentioning

confidence: 99%

Effect of Selenium on the Growth and Lipid Accumulation of Yarrowia lipolytica Yeast

Kieliszek

Dourou

2020

Biol Trace Elem Res

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Nowadays, there is an increase attention on the effect of selenium (Se) on metabolic processes of microorganisms. Strains belonging to the genus of Yarrowia are of great biotechnological interest for various industries. In this study, we evaluated the effect of 10 mg/L of Se on the growth and lipid production of two Yarrowia lipolytica strains: the ACA DC 50109 and one more with increased oleagenicity, derived after ALE methodology (referred here as Y. lipolytica ALE_70). The presence of Se in the growth medium negatively affected both cell mass production and total lipid accumulation, for both Y. lipolytica strains. Fractionation of total lipids showed an inhibition on neutral lipid (NL) synthesis and consequently, an increase of polar lipids (glycolipids plus sphingolipids, and phospholipids) on the lipids of the Se-enriched ACA DC 50109 strain; however, the NL/polar ratio of the Se-enriched ALE_70 indicated that Se, apart from the inhibition of NL synthesis, provoked also the accumulation of polar lipids in this strain. In addition, the fatty acid (FA) composition was differently affected by Se. Se-enriched total lipids of the ALE_70 strain were enriched in linoleic acid (C18:2 n-6), which resulted in increase of the unsaturated index. On the other hand, Se-enriched lipids of the ACA DC 50109 strain were more saturated, as the percentage of palmitic (C16:0) and stearic (C18:0) acids increased in the total FAs. Moreover, it seems that Se influenced the activity or the expression of desaturases and elongase in both strains. Finally, the supplementation of growth medium with Se affected cell morphology, as well as the size and distribution of lipid droplets inside the yeast cells. According to our opinion, Se caused stress conditions and the consequence of that was the occurrence of metabolic disorders that affected cell mass, lipid content, and/or morphological structures. The results of the present study suggest that further research should be carried out to understand the background of the lipogenesis process in yeast cells cultured under stress conditions.

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Selenium nanoparticle-enriched biomass of Yarrowia lipolytica enhances growth and survival of Artemia salina

Cited by 27 publications

References 52 publications

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production

Effect of Selenium on the Growth and Lipid Accumulation of Yarrowia lipolytica Yeast

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