Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Kieliszek, Marek; Bierła, Katarzyna; Jiménez-Lamana, Javier; Kot, Anna M.; Alcántara-Durán, Jaime; Piwowarek, Kamil; Błażejak, Stanisław; Szpunar, Joanna

doi:10.3390/ijms21155287

Cited by 30 publications

(12 citation statements)

References 68 publications

(110 reference statements)

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“…The chemical form of selenium is an important factor influencing biological activity. This non-metal occurs in an inorganic form in certain forms of selenite(IV) and selenate(VI), while in the organic form, it occurs in certain amino acids, e.g., selenocysteine and selenomethionine [ 44 , 45 ].…”

Section: Selenium As An Elementmentioning

confidence: 99%

Selenium in the Prevention of SARS-CoV-2 and Other Viruses

Kieliszek

2022

Biol Trace Elem Res

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The rapid spread of new pathogens (SARS-CoV-2 virus) that negatively affect the human body has huge consequences for the global public health system and the development of the global economy. Appropriate implementation of new safety regulations will improve the functioning of the current model supervising the inhibition of the spread of COVID-19 disease. Compliance with all these standards will have a key impact on the health behavior of individual social groups. There have been demonstrably effective treatments that proved to be effective but were rapidly dismissed for unknown reasons, such as ivermectin and hydroxychloroquine. Various measures are used in the world to help inhibit its development. The properties of this element provide hope in preventing the development of the SARS-CoV-2 virus. The aim of this review is to synthesize the latest literature data and to present the effect of sodium selenite in reducing the incidence of COVID-19 disease.

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Section: Selenium As An Elementmentioning

confidence: 99%

Selenium in the Prevention of SARS-CoV-2 and Other Viruses

Kieliszek

2022

Biol Trace Elem Res

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“…It is well-documented that high trehalose level correlates with increased tolerance to various stresses in yeast [17,20,21,25,65,84] and other fungi, as well as in prokaryotes, nematodes and plants [85][86][87]. Because stressors, like high temperature, desiccation, high doses of alcohol, oxidants or selenium supplementation [88], all affect membrane integrity, the disaccharide can be considered as a universal stress-responsive membrane protectant. By using the fission yeast S. pombe as a model organism, here we studied the heat-induced crosstalk between trehalose and lipid metabolism for the first time.…”

Section: Discussionmentioning

confidence: 99%

Lipids and Trehalose Actively Cooperate in Heat Stress Management of Schizosaccharomyces pombe

Péter

Gudmann

Kóta

et al. 2021

IJMS

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Homeostatic maintenance of the physicochemical properties of cellular membranes is essential for life. In yeast, trehalose accumulation and lipid remodeling enable rapid adaptation to perturbations, but their crosstalk was not investigated. Here we report about the first in-depth, mass spectrometry-based lipidomic analysis on heat-stressed Schizosaccharomyces pombe mutants which are unable to synthesize (tps1Δ) or degrade (ntp1Δ) trehalose. Our experiments provide data about the role of trehalose as a membrane protectant in heat stress. We show that under conditions of trehalose deficiency, heat stress induced a comprehensive, distinctively high-degree lipidome reshaping in which structural, signaling and storage lipids acted in concert. In the absence of trehalose, membrane lipid remodeling was more pronounced and increased with increasing stress dose. It could be characterized by decreasing unsaturation and increasing acyl chain length, and required de novo synthesis of stearic acid (18:0) and very long-chain fatty acids to serve membrane rigidification. In addition, we detected enhanced and sustained signaling lipid generation to ensure transient cell cycle arrest as well as more intense triglyceride synthesis to accommodate membrane lipid-derived oleic acid (18:1) and newly synthesized but unused fatty acids. We also demonstrate that these changes were able to partially substitute for the missing role of trehalose and conferred measurable stress tolerance to fission yeast cells.

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“…Thus, a conversion of the inorganic Se into the organic Se via various chemical reactions and microorganisms had been investigated. For example, both proteins and polysaccharides could be selenylated [ 12 , 13 ], while yeast could be employed to convert the inorganic Se into the organic Se [ 14 ]. Subsequently, the Se-enriched yeast was found to be able to improve the cognitive decline in the model mice and reduce the Aβ and tua lesions in the brain tissue [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Barrier-Enhancing Function of Soluble Yam (Dioscorea opposita Thunb.) Polysaccharides in Rat Intestinal Epithelial Cells as Affected by the Covalent Se Conjugation

Wang

Zhao

2022

Nutrients

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The non-starch yam polysaccharides (YP) are the bioactive substances of edible yam, while Se is an essential nutrient for the human body. Whether a covalent conjugation of Se to YP might cause bioactivity change for the resultant selenylated YP in the intestine is still insufficiently studied, including the critical intestinal barrier function. In this study, two selenylated YP products, namely, YPSe-I and YPSe-II, with corresponding Se contents of 795 and 1480 mg/kg, were obtained by the reaction of YP and Na2SeO3 in the presence of HNO3 and then assessed for their bioactivities to a cell model (i.e., rat intestinal epithelial IEC-6 cells). The results showed that YP, YPSe-I, and YPSe-II at 5–80 μg/mL dosages could promote cell growth with treatment times of 12–24 h. The three samples also could improve barrier integrity via increasing cell monolayer resistance and anti-bacterial activity against E. coli or by reducing paracellular permeability and bacterial translocation. Additionally, the three samples enhanced F-actin distribution and promoted the expression of the three tight junction proteins, namely, zonula occluden-1, occludin, and claudin-1. Meanwhile, the expression levels of ROCK and RhoA, two critical proteins in the ROCK/RhoA singling pathway, were down-regulated by these samples. Collectively, YPSe-I and, especially, YPSe-II were more potent than YP in enhancing the assessed bioactivities. It is thus concluded that this chemical selenylation of YP brought about enhanced activity in the cells to promote barrier integrity, while a higher selenylation extent of the selenylated YP induced much activity enhancement. Collectively, the results highlighted the important role of the non-metal nutrient Se in the modified polysaccharides.

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Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium

Cited by 30 publications

References 68 publications

Selenium in the Prevention of SARS-CoV-2 and Other Viruses

Selenium in the Prevention of SARS-CoV-2 and Other Viruses

Lipids and Trehalose Actively Cooperate in Heat Stress Management of Schizosaccharomyces pombe

The Barrier-Enhancing Function of Soluble Yam (Dioscorea opposita Thunb.) Polysaccharides in Rat Intestinal Epithelial Cells as Affected by the Covalent Se Conjugation

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