Selenium metabolomics in yeast using complementary reversed-phase/hydrophilic ion interaction (HILIC) liquid chromatography–electrospray hybrid quadrupole trap/Orbitrap mass spectrometry

Arnaudguilhem, Carine; Bierła, Katarzyna; Ouerdane, Laurent; Preud’homme, Hugues; Yiannikouris, Alexandros; Łobiński, Ryszard

doi:10.1016/j.aca.2012.10.029

Cited by 80 publications

(66 citation statements)

References 31 publications

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“…Selenols react with thiols leading to the production of selenylsulfides and disulfides (51). These reactions are believed to play a central role in selenium toxicity by reducing intracellular GSH levels and amplifying redox imbalance caused by ROS (52).…”

Section: Discussionmentioning

confidence: 99%

Trans-sulfuration Pathway Seleno-amino Acids Are Mediators of Selenomethionine Toxicity in Saccharomyces cerevisiae*

Lazard

Dauplais

Blanquet

et al. 2015

Journal of Biological Chemistry

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Background:The mechanisms underlying selenomethionine toxicity are poorly understood. Results: Saccharomyces cerevisiae mutations affecting sulfur metabolism or superoxide degradation impact selenomethionine toxicity. Conclusion: Selenomethionine primarily exerts its toxicity via the seleno-amino acids selenohomocysteine and selenocysteine. Significance: This study highlights the as yet underestimated importance of the trans-sulfuration pathway in selenomethionine toxicity.

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Section: Discussionmentioning

confidence: 99%

Trans-sulfuration Pathway Seleno-amino Acids Are Mediators of Selenomethionine Toxicity in Saccharomyces cerevisiae*

Lazard

Dauplais

Blanquet

et al. 2015

Journal of Biological Chemistry

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“…In the reaction catalyzed by S-adenozylomethionine synthase, selenomethionine (SeMet) is converted to Se-adenosyl-selenomethionine (SeAM). In the subsequent reaction, SeAM is subjected to enzymatic methylation process; as a result of which, adenosyl homo-seleno cysteine (SeAHCys) is released (Arnaudguilhem et al 2012). Subsequently, the resulting compound undergoes hydrolysis, which results in the formation of selenohomocysteine (SeHCys) (Kitajima and Chiba 2013).…”

Section: Metabolism Of Selenium By Yeast Cellsmentioning

confidence: 99%

Accumulation and metabolism of selenium by yeast cells

Kieliszek

Błażejak

Gientka

et al. 2015

Appl Microbiol Biotechnol

162

130

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This paper examines the process of selenium bioaccumulation and selenium metabolism in yeast cells. Yeast cells can bind elements in ionic from the environment and permanently integrate them into their cellular structure. Up to now, Saccharomyces cerevisiae, Candida utilis, and Yarrowia lipolytica yeasts have been used primarily in biotechnological studies to evaluate binding of minerals. Yeast cells are able to bind selenium in the form of both organic and inorganic compounds. The process of bioaccumulation of selenium by microorganisms occurs through two mechanisms: extracellular binding by ligands of membrane assembly and intracellular accumulation associated with the transport of ions across the cytoplasmic membrane into the cell interior. During intracellular metabolism of selenium, oxidation, reduction, methylation, and selenoprotein synthesis processes are involved, as exemplified by detoxification processes that allow yeasts to survive under culture conditions involving the elevated selenium concentrations which were observed. Selenium yeasts represent probably the best absorbed form of this element. In turn, in terms of wide application, the inclusion of yeast with accumulated selenium may aid in lessening selenium deficiency in a diet.

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“…Metabolization of seleno-compounds in vivo gives rise to multiple different metabolites. For example, more than 60 low-molecular-weight different selenium-metabolites were found in Se-enriched yeast grown in a medium containing selenite (55). Therefore, deciphering the effects of a given metabolite is a major difficulty in selenium studies, which can be overcome by using yeast strains deleted for individual genes in a particular pathway.…”

Section: Saccharomyces Cerevisiae As An Experimental Model For the Stmentioning

confidence: 99%

Recent advances in the mechanism of selenoamino acids toxicity in eukaryotic cells

Lazard

Dauplais

Blanquet

et al. 2017

Biomolecular Concepts

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Selenium is an essential trace element due to its incorporation into selenoproteins with important biological functions. However, at high doses it is toxic. Selenium toxicity is generally attributed to the induction of oxidative stress. However, it has become apparent that the mode of action of seleno-compounds varies, depending on its chemical form and speciation. Recent studies in various eukaryotic systems, in particular the model organism Saccharomyces cerevisiae, provide new insights on the cytotoxic mechanisms of selenomethionine and selenocysteine. This review first summarizes current knowledge on reactive oxygen species (ROS)-induced genotoxicity of inorganic selenium species. Then, we discuss recent advances on our understanding of the molecular mechanisms of selenocysteine and selenomethionine cytotoxicity. We present evidences indicating that both oxidative stress and ROSindependent mechanisms contribute to selenoamino acids cytotoxicity. These latter mechanisms include disruption of protein homeostasis by selenocysteine misincorporation in proteins and/or reaction of selenols with protein thiols.

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Selenium metabolomics in yeast using complementary reversed-phase/hydrophilic ion interaction (HILIC) liquid chromatography–electrospray hybrid quadrupole trap/Orbitrap mass spectrometry

Cited by 80 publications

References 31 publications

Trans-sulfuration Pathway Seleno-amino Acids Are Mediators of Selenomethionine Toxicity in Saccharomyces cerevisiae*

Trans-sulfuration Pathway Seleno-amino Acids Are Mediators of Selenomethionine Toxicity in Saccharomyces cerevisiae*

Accumulation and metabolism of selenium by yeast cells

Recent advances in the mechanism of selenoamino acids toxicity in eukaryotic cells

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