Selenium-doped hydroxyapatite nanoparticles for potential application in bone tumor therapy

Barbanente, Alessandra; Palazzo, Barbara; Esposti, Lorenzo Degli; Adamiano, Alessio; Iafisco, Michele; Ditaranto, Nicoletta; Migoni, Danilo; Gervaso, Francesca; Nadar, Robin A.; Ivanchenko, Pavlo; Leeuwenburgh, Sander C.G.; Margiotta, Nicola

doi:10.1016/j.jinorgbio.2020.111334

Cited by 29 publications

(15 citation statements)

References 53 publications

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“…Our results suggest that only the CaP_Se1 powder, with low concentration of Se, show selective toxicity to cancer cells (U2OS), without harming non-tumorigenic cells (HEK 293) cells. Results are consistent with the study of Barbanente et al [ 20 ], who evaluated the cytotoxic activity of Se-doped HAp nano particles using prostate and breast cancer cells as well as healthy human bone marrow stem cells. They found that nanoparticles with a high concentration of Se showed a strong anticancer effect but also caused a significant increase in toxicity towards normal cells.…”

Section: Resultssupporting

confidence: 92%

“…The difference between the c parameters of non-substituted and Se-substituted HAps is smaller for higher Se-substitution levels, where the higher content of Na + ions was detected, indicating additional charge defect compensation by Na-substitution for Ca according to the formula Ca 10- x Na x (PO 4 ) 6- x (SeO 3 ) x (OH) 2 . The lattice parameters of substituted samples compare fairly well with the literature data reported by Barbanente et al [ 20 ], showing an almost linear rise of cell parameters with Se substitution up to a Se/(P + Se) molar ratio of 0.1 ( Figure 2 ). Wei et al [ 10 ] reported that a and b lattice parameters increase along with the Se-substitution level, while lattice parameter c decreases, while Sun et al [ 21 ] reported a non-continuous change of a and b and decrease of c lattice parameters.…”

Section: Resultssupporting

confidence: 88%

“… Unit cell parameters of HAp phase as a function of Se/(P + Se) molar ratio in as-prepared powders (black circles) compared to the literature data [ 20 ] (empty circles). …”

Section: Figurementioning

confidence: 99%

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Selenite Substituted Calcium Phosphates: Preparation, Characterization, and Cytotoxic Activity

et al. 2021

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The aim of this study was to prepare a biomimetic selenium substituted calcium phosphate system for potential application in osteosarcoma therapy. Calcium phosphate (CaP) systems substituted with selenite ions were prepared by the wet precipitation method, using biogenic CaCO3 (derived from cuttlefish bone), CO(NH2)2-H3PO4, and Na2SeO3·5H2O as reagents. Starting reaction mixtures were prepared based on the formula for selenite-substituted hydroxyapatite, Ca10(PO4)6-x(SeO3)x(OH)2, with Ca/(P + Se) molar ratio of 1.67 and Se/(P + Se) molar ratio of: 0, 0.01, 0.05, and 0.10, respectively. The prepared CaP powders were characterized by Fourier transform infrared spectrometry, elemental analysis, scanning electron microscopy, X-ray powder diffraction analysis and Rietveld refinement studies. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37 °C. The metabolic activity of healthy and osteosarcoma cell lines was assessed by cell cytotoxicity and viability test. The as-prepared powders were composed of calcium-deficient carbonated hydroxyapatite (HAp), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). Along with the selenite substitution, the presence of Sr2+, Na+, and Mg2+ was detected as a result of using cuttlefish bone as a precursor for Ca2+ ions. Inductively coupled plasma mass spectrometry analysis showed that the Se/(P + Se) molar ratios of selenite substituted powders are lower than the nominal ratios. Heat treated powders were composed of HAp, α-tricalcium phosphate (α-TCP) and β-tricalcium phosphate (β-TCP). Doping CaP structure with selenite ions improves the thermal stability of HAp. The powder with the Se/(P + Se) molar ratio of 0.007 showed selective toxicity to cancer cells.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 88%

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Selenite Substituted Calcium Phosphates: Preparation, Characterization, and Cytotoxic Activity

et al. 2021

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“…This is in agreement with previous studies conducted by Bao et al [ 38 ] and Zhou et al [ 39 ] that showed Se-induced cytotoxicity to cancer cells. Moreover, previous studies on selenium-containing hydroxyapatite and its anti-tumour properties clearly show that the key is the appropriate amount of selenium in the materials: unfortunately, too high concentration of selenium is toxic to healthy cells [ 40 ]. Therefore, the concentration of Se should be selected in such a way as to ensure effective activity against osteosarcoma cells while being non-toxic to osteoblasts.…”

Section: Resultsmentioning

confidence: 99%

Porous Composite Granules with Potential Function of Bone Substitute and Simvastatin Releasing System: A Preliminary Study

2021

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In this work, 3D porous granules based on Zn and Se-containing calcium phosphates (CaPs) were fabricated using a droplet-extrusion technique. The composite beads varied in composition and contained two different natural polymers: sodium alginate (SA) and gelatin (GEL). To analyse and compare their physicochemical properties, such as porosity and morphology, different techniques were applied, including scanning electron microscopy (SEM), sorption of N2 and mercury porosimetry. Prior to the fabrication of the granules, the properties of CaPs materials, (the bioceramic base of the beads), selenium (IV)-substituted hydroxyapatite (Se-HA) and zinc-substituted dicalcium phosphate dihydrate (Zn-DCPD), were also investigated. The results of cell viability assessment showed that Se-HA powder was non-toxic to human osteoblasts (hFOB 1.19) and simultaneously exhibited high toxicity to tumour cells (Saos-2). Once the cytotoxicity assay was completed, Se-HA and Zn-DCPD were used to prepare 3D materials. The prepared porous granules were used as matrices to deliver simvastatin to bones. Simvastatin was applied in either the lipophilic form or hydrophilic form. The release kinetics of simvastatin from granules of different composition was then assessed and compared.

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“…However, due to the relatively complex material preparation and characterization process, a limited number of publications have reported the use of selenium for 3D scaffolds. Most studies focused on the incorporation of selenium into ceramic, polymeric, and metallic nanoparticles, as well as a coating of metallic substrate [122][123][124][125][126][127][128][129][130][131][132][133][134][135][136][137]. These studies suggested that selenium is an effective functional material that can be used to treat bone cancer problems without affecting normal cells, also promoting bone regeneration, which is related to its antioxidant and prooxidant properties.…”

Section: Scaffolds With Advanced Functional Materialsmentioning

confidence: 99%

Application of additively manufactured 3D scaffolds for bone cancer treatment: a review

2022

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Bone cancer is a critical health problem on a global scale, and the associated huge clinical and economic burdens are still rising. Although many clinical approaches are currently used for bone cancer treatment, these methods usually affect the normal body functions and thus present significant limitations. Meanwhile, advanced materials and additive manufacturing have opened up promising avenues for the development of new strategies targeting both bone cancer treatment and post-treatment bone regeneration. This paper presents a comprehensive review of bone cancer and its current treatment methods, particularly focusing on a number of advanced strategies such as scaffolds based on advanced functional materials, drug-loaded scaffolds, and scaffolds for photothermal/magnetothermal therapy. Finally, the main research challenges and future perspectives are elaborated.

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Selenium-doped hydroxyapatite nanoparticles for potential application in bone tumor therapy

Cited by 29 publications

References 53 publications

Selenite Substituted Calcium Phosphates: Preparation, Characterization, and Cytotoxic Activity

Selenite Substituted Calcium Phosphates: Preparation, Characterization, and Cytotoxic Activity

Porous Composite Granules with Potential Function of Bone Substitute and Simvastatin Releasing System: A Preliminary Study

Application of additively manufactured 3D scaffolds for bone cancer treatment: a review

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