Selenium-Binding Protein 1 expression in ovaries and ovarian tumors in the laying hen, a spontaneous model of human ovarian cancer

Abstract

Objective-Reduced Selenium-Binding Protein 1 (SELENBP1) expression was recently shown in multiple cancers. There is little information on the expression and function of SELENBP1 in cancer progression. In order to develop a better understanding of the role of SELENBP1 in ovarian cancer, our objective was to determine if SELENBP1 is expressed in the normal ovaries and ovarian tumors in the egg-laying hen, a spontaneous model of human ovarian cancer.Methods-SPB1 mRNA expression in normal ovary (n=20) and ovarian tumors (n=23) was evaluated by RT-PCR. Relative levels of mRNA were compared by quantitative RT-PCR (qRT-PCR) in selected samples. SELENBP1 protein expression was evaluated by 1D Western Blot and immunohistochemistry with a commercial anti-human SELENBP1 antibody.Results-SELENBP1 mRNA and protein was expressed in 100% of normal and ovarian tumors and qRT-PCR confirmed decreased mRNA expression in 80% of ovarian tumors. SELENBP1 was primarily localized in surface epithelial cells of normal ovaries. In ovaries containing early tumor lesions, SELENBP1 expression was reduced in the surface epithelium near the tumor and was expressed in tumor cells, while more distant regions with normal histology retained SELENBP1 expression in the surface epithelium.Conclusions-We have shown for the first time that SELENBP1 is expressed in both normal ovaries and ovarian tumors in the hen and that SELENBP1 expression is altered in the vicinity of the tumor. Furthermore, SELENBP1 expression in normal ovarian surface epithelium and in ovarian tumors parallels that previously reported for ovarian cancer in women.

Progressive loss of selenium-binding protein 1 expression correlates with increasing epithelial proliferation and papillary complexity in ovarian serous borderline tumor and low-grade serous carcinoma

“…We were able to demonstrate a differential expression of SELENBP1 in various histologic components within SBOT. Although a decreased expression of SELENBP1 was previously described in high-grade ovarian serous carcinoma of the human subjects [4] and of the experimental hens [11], our study showed a reduction of SELENBP1 expression in LGSC of the human ovary.…”

“…By membrane proteome profiling analysis, SELENBP1 was found to be the most significantly down-regulated protein in ovarian cancer cells [4]. Down-regulation of SELENBP1 was also demonstrated in ovarian carcinomas in the experimental laying hens [11]. To date, however, no systematic studies have been carried out concerning the expressions of SELENBP1 in SBOT, M-SBOT, and LGSC.…”

Arabidopsis Putative Selenium-Binding Protein1 Expression Is Tightly Linked to Cellular Sulfur Demand and Can Reduce Sensitivity to Stresses Requiring Glutathione for Tolerance

“…Although a physiological function has not yet been assigned to SBP1, its involvement in detoxification mechanisms is largely suggested. Downregulation of SBP1 expression is correlated with rapid tumor development in many organs (Kim et al, 2006;Stammer et al, 2008), and recently, SBP1 was characterized as a biomarker for schizophrenia (Glatt et al, 2005). Moreover, its homolog SBP2 is suggested to play a protective role as a scavenger of toxic electrophiles or oxidant species (Lanfear et al, 1993;Cohen et al, 1997;Mattow et al, 2006).…”

Selenium-Binding Protein 1 (SBP1) autoantibodies in ovarian disorders and ovarian cancer

“…The histology, biochemistry, genetic mutations, and tumor immunology in this model have striking similarities with human ovarian tumors (Rodriguez-Burford et al 2001, Barua et al 2009a, Barua et al 2009b, Hakim et al 2009, Gonzalez Bosquet et al 2010, Bradaric et al 2013). Both anti-SBP1 (Stammer et al 2008) and anti-mesothelin (Yu et al 2011) are associated with reduced ovarian function and with ovarian tumors in the hen.…”