Selenium and Cadmium Tissue Concentrations and the CASA Sperm Motility Analysis after Administration to Rats

Toman, R.; Hluchý, Svätoslav; Massányi, Peter; Lukáč, Norbert; Adamkovičová, Mária; Cabaj, Michal; Hájková, Zuzana

doi:10.3844/ajavsp.2014.194.202

Cited by 3 publications

(2 citation statements)

References 66 publications

(77 reference statements)

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“…Sperm motility is needed for the sperm to reach the cervix and fallopian tubes, and unite with the oocyte. Thus, a decrease in sperm motility is considered an important factor of chemical-induced infertility [ 29 , 35 ]. Sperm morphology development is another important parameter in determining semen quality, and continues into the late stages of spermatogenesis [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress

et al. 2017

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Levetiracetam (LEV) is an antiepileptic drug commonly used in the treatment of epilepsy because of its excellent safety profile in all age groups. It is remarkable that there are no studies evaluating the toxic effects of this drug on the male reproductive system, as it is commonly used in male patients of reproductive age. From this point of view, our aim was to evaluate the possible toxic effects of LEV on the male reproductive system. Therefore, LEV was administered to male rats orally at 50, 150, and 300 mg/kg for 70 consecutive days. At the end of this period, alterations to body and organ weights were calculated, and sperm concentration, motility, and morphology were investigated by a computer-assisted sperm analysis system. Sperm DNA damage was determined by comet assay and histopathological examination of the testes was carried out. Serum testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels were measured by ELISAs to determine the effects of hormonal status, while glutathione, superoxide dismutase, catalase, and malondialdehyde levels in the testes were measured by colorimetric assay kits to determine the role of oxidative status in potential toxicity. According to the results, sperm quality was decreased by LEV treatment in a dose-dependent manner. LEV induced significant DNA damage in the 150 and 300 mg/kg LEV-administered groups. Histopathology of the testes showed that LEV resulted in testicular injury in the 300 mg/kg LEV-administered group. Serum testosterone, FSH, and LH levels were significantly decreased in the 300 mg/kg LEV-administered group. Glutathione, superoxide dismutase, and catalase levels were significantly decreased in all experimental groups while malondialdehyde levels were significantly increased in 150 and 300 mg/kg LEV-administered groups. According to these results, it was determined that LEV administration decreased sperm quality and it was alleged that hormonal alteration and oxidative stress are potential contributors to reproductive toxicity.

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Section: Discussionmentioning

confidence: 99%

Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress

et al. 2017

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“…Cu and Zn were determined by a flame atomic absorption spectrometer (FAAS) using an ethane-air flame (CAAM-2001N) [ 30 ], while Hg was determined by cold-vapor atomic absorption spectrometry (CVAAS) (Ultima Expert) [ 31 ]. Cd and Cr were measured using an electrothermal atomic absorption spectrometer (ETAAS) (Avio 200) [ 32 ], while As and Se were analysed using the hydride generation method (HGAAS) (AA-6033C) [ 33 ]. The accuracy of the analytical data were ensured by triplicate samples, blank test controls and careful standardization.…”

Section: Methodsmentioning

confidence: 99%

Water quality assessment and source identification of the Shuangji River (China) using multivariate statistical methods

et al. 2021

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Multivariate statistical techniques, including cluster analysis (CA), discriminant analysis (DA), principal component analysis (PCA) and factor analysis (FA), were used to evaluate temporal and spatial variations in and to interpret large and complex water quality datasets collected from the Shuangji River Basin. The datasets, which contained 19 parameters, were generated during the 2 year (2018–2020) monitoring programme at 14 different sites (3192 observations) along the river. Hierarchical CA was used to divide the twelve months into three periods and the fourteen sampling sites into three groups. Discriminant analysis identified four parameters (CODMn, Cu, As, Se) loading more than 68% correct assignations in temporal analysis, while seven parameters (COD, TP, CODMn, F, LAS, Cu and Cd) to load 93% correct assignations in spatial analysis. The FA/PCA identified six factors that were responsible for explaining the data structure of 68% of the total variance of the dataset, allowing grouping of selected parameters based on common characteristics and assessing the incidence of overall change in each group. This study proposes the necessity and practicality of multivariate statistical techniques for evaluating and interpreting large and complex data sets, with a view to obtaining better information about water quality and the design of monitoring networks to effectively manage water resources.

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How the Pregabalin (Lyrica) Administration as an Abuse Drug Can Affects the Reproductive Health of Male Wistar Rat

Alduweesh¹

2021

IJPSRR

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Drugs addiction considered a massive problem persist in diverse populations everywhere the world. Our work aimed to illustrate the correlation between drug addiction and reproductive function in an animal model, also the potential impact of pregabalin (Lyrica) intake on spermatozoa formation process and sexual hormone levels. In this study, we used 14 adult healthy rats that allocated into two groups (n = 7) as control and treated that orally administrated with pregabalin (23.7 mg/kg) for 30 subsequent days. Reproductive hormones, spermatozoa parameters (motility and morphology), lipid peroxidation (MDA), nitric oxide, total antioxidant activity, DNA damage and histopathological investigation were performed. The results revealed that pregabalin addiction had a harmful impact on the hypothalamus-pituitary-gonad axis of male rats through hindered hormones secretion, raised reactive oxygen species, affect antioxidant enzymes, triggered DNA damage and distorted testicular histology. Finally, we found that addiction of Lyrica caused adverse impact on the male reproductive health and subsequently affect fertility.

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Selenium and Cadmium Tissue Concentrations and the CASA Sperm Motility Analysis after Administration to Rats

Cited by 3 publications

References 66 publications

Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress

Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress

Water quality assessment and source identification of the Shuangji River (China) using multivariate statistical methods

How the Pregabalin (Lyrica) Administration as an Abuse Drug Can Affects the Reproductive Health of Male Wistar Rat

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